公开(公告)号：US08273562B2

公开(公告)日：2012-09-25

申请号：US13344110

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov, legal representative ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C12Q1/68 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US08114651B2

公开(公告)日：2012-02-14

申请号：US12412823

申请日：2009-03-27

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov, legal representative ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。

公开(公告)号：US20120107883A1

公开(公告)日：2012-05-03

申请号：US13344170

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US20120107884A1

公开(公告)日：2012-05-03

申请号：US13344200

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。

公开(公告)号：US20120115203A1

公开(公告)日：2012-05-10

申请号：US13344110

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi ,  Vitaly Grigorievich Paraskevov

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi ,  Vitaly Grigorievich Paraskevov

IPC分类号： C12N9/02 ,  C12N1/15 ,  C12N1/21 ,  C12N15/53 ,  C12N15/63

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US20090275092A1

公开(公告)日：2009-11-05

申请号：US12412823

申请日：2009-03-27

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorlevich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorlevich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06 ,  C12N9/02 ,  C07H21/04 ,  C12N1/00 ,  C07K14/00

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。

公开(公告)号：US08367381B2

公开(公告)日：2013-02-05

申请号：US13344170

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi ,  Vitaly Grigorievich Paraskevov

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06 ,  C12P21/06 ,  C12P21/04 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/26 ,  C07H21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。

公开(公告)号：US08367382B2

公开(公告)日：2013-02-05

申请号：US13344200

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi ,  Vitaly Grigorievich Paraskevov

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06 ,  C12P21/06 ,  C12P21/04 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/26 ,  C07H21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US07927843B2

公开(公告)日：2011-04-19

申请号：US11878558

申请日：2007-07-25

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.

摘要翻译： 本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸盐的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法， 微生物。 本发明还公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸盐的微生物本身存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者 源于微生物的酶。

公开(公告)号：US20070298468A1

公开(公告)日：2007-12-27

申请号：US11878558

申请日：2007-07-25

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.