Promoter and terminator sequences of formate dehydrogenase gene of
Candida boidinii
    1.
    发明授权
    Promoter and terminator sequences of formate dehydrogenase gene of Candida boidinii 失效
    博伊丁假丝酵母的甲酸脱氢酶基因的启动子和终止子序列

    公开(公告)号:US06001590A

    公开(公告)日:1999-12-14

    申请号:US817926

    申请日:1997-05-09

    CPC分类号: C12N9/0008 C12N15/815

    摘要: A promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising a 190 bp or more continuous nucleotide sequence selected from the nucleotide sequence of SEQ ID NO:1; a promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:1, 48, 49 or 50; a terminator for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:2; a gene expression cassette comprising said promoter, a heterologous gene and said terminator; a recombinant expression vector comprising said gene expression cassette; a transformant transformed with said recombinant expression vector; a process for producing an expression product of a heterologous gene, which comprises culturing said transformant and recovering an expression product of a heterologous gene from the culture.

    摘要翻译: PCT No.PCT / JP96 / 02597 371日期:1997年5月9日 102(e)日期1997年5月9日PCT提交1996年9月12日PCT公布。 公开号WO97 / 10345 日期:1997年3月20日来自布氏念珠菌的甲酸脱氢酶基因的启动子,基本上包含选自SEQ ID NO:1的核苷酸序列的190bp以上的连续核苷酸序列; 来自布氏念珠菌的甲酸脱氢酶基因的启动子,基本上包含SEQ ID NO:1,49,49或50的核苷酸序列; 来自布氏念珠菌的甲酸脱氢酶基因的终止子,基本上包含SEQ ID NO:2的核苷酸序列; 包含所述启动子,异源基因和所述终止子的基因表达盒; 包含所述基因表达盒的重组表达载体; 用所述重组表达载体转化的转化体; 用于产生异源基因的表达产物的方法,其包括培养所述转化体并从培养物中回收异源基因的表达产物。