公开(公告)号：US11807956B2

公开(公告)日：2023-11-07

申请号：US17068551

申请日：2020-10-12

申请人： Twist Bioscience Corporation

发明人： Anthony Cox ,  Sebastian Treusch ,  Siyuan Chen

IPC分类号： C40B50/06 ,  C12N15/10 ,  G01N33/50 ,  C40B50/14 ,  C40B50/08 ,  C12N15/113 ,  C12Q1/6876 ,  C12Q1/6886

CPC分类号： C40B50/06 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6886 ,  C12Q2600/158

摘要： Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US10053688B2

公开(公告)日：2018-08-21

申请号：US15682100

申请日：2017-08-21

申请人： Twist Bioscience Corporation

发明人： Anthony Cox ,  Siyuan Chen

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C40B40/06 ,  C12Q1/6806 ,  C12Q1/6816

CPC分类号： C12N15/1068 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q1/6816 ,  C40B40/06

摘要： Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US10975372B2

公开(公告)日：2021-04-13

申请号：US16031784

申请日：2018-07-10

申请人： TWIST BIOSCIENCE CORPORATION

发明人： Anthony Cox ,  Siyuan Chen

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C40B50/14 ,  C12Q1/6816 ,  C40B40/06 ,  C12N15/11

摘要： Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US10907274B2

公开(公告)日：2021-02-02

申请号：US15844395

申请日：2017-12-15

申请人： Twist Bioscience Corporation

发明人： Anthony Cox ,  Siyuan Chen

IPC分类号： C40B40/08 ,  C07K14/725 ,  G01N33/574 ,  G01N33/68 ,  C12N15/10 ,  G01N33/50 ,  C40B30/04 ,  A61K39/00

摘要： Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a TCR or a TCR-binding antigen. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

公开(公告)号：US20180312834A1

公开(公告)日：2018-11-01

申请号：US16031784

申请日：2018-07-10

申请人： TWIST BIOSCIENCE CORPORATION

发明人： Anthony Cox ,  Siyuan Chen

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6816 ,  C40B40/06

CPC分类号： C12N15/1068 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q1/6816 ,  C40B40/06

摘要： Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US10894959B2

公开(公告)日：2021-01-19

申请号：US15921537

申请日：2018-03-14

申请人： TWIST BIOSCIENCE CORPORATION

发明人： Anthony Cox ,  Siyuan Chen

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.