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公开(公告)号：US11807956B2
公开(公告)日：2023-11-07
申请号：US17068551
申请日：2020-10-12
Applicant: Twist Bioscience Corporation
Inventor： Anthony Cox , Sebastian Treusch , Siyuan Chen
IPC: C40B50/06 , C12N15/10 , G01N33/50 , C40B50/14 , C40B50/08 , C12N15/113 , C12Q1/6876 , C12Q1/6886
CPC classification number: C40B50/06 , C12N15/1079 , C12N15/1093 , C12N15/113 , C12Q1/6876 , C40B50/08 , C40B50/14 , G01N33/502 , G01N33/5029 , G01N33/5041 , C12N2320/30 , C12N2330/31 , C12Q1/6886 , C12Q2600/158
Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.
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公开(公告)号：US11377676B2
公开(公告)日：2022-07-05
申请号：US16712678
申请日：2019-12-12
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Cheng-Hsien Wu , Sebastian Treusch
IPC: C12P19/34
Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.
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公开(公告)号：US20210040476A1
公开(公告)日：2021-02-11
申请号：US17068551
申请日：2020-10-12
Applicant: Twist Bioscience Corporation
Inventor： Anthony COX , Sebastian Treusch , Siyuan CHEN
IPC: C12N15/10 , G01N33/50 , C40B50/14 , C40B50/08 , C12N15/113 , C12Q1/6876
Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.
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公开(公告)号：US09677067B2
公开(公告)日：2017-06-13
申请号：US15154879
申请日：2016-05-13
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Esteban Toro , Sebastian Treusch , Siyuan Chen , Cheng-Hsien Wu
CPC classification number: C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/66 , C12P19/34
Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.
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公开(公告)号：US20160264958A1
公开(公告)日：2016-09-15
申请号：US15154879
申请日：2016-05-13
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Esteban Toro , Sebastian Treusch , Siyuan Chen , Cheng-Hsien Wu
IPC: C12N15/10
CPC classification number: C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/66 , C12P19/34
Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.
Abstract translation: 提供了用于组装大核酸的方法和组合物，其中组装的大核酸在组装过程中缺少内部序列修饰。
