公开(公告)号：US20200308619A1

公开(公告)日：2020-10-01

申请号：US16643823

申请日：2018-08-30

Applicant: UNIVERSIDAD DE VALPARAISO

Inventor： Alejandro DINAMARCA ,  Natalia ROMO ,  Claudia IBACACHE

IPC: C12Q1/02

Abstract: The invention relates to a gene construct for detecting the presence of microorganisms that produce chemical molecules of the Acyl-homoserine lactone (AHL) type, comprising a first expression cassette that includes a copper-inducible promoter operably linked to the gene encoding the RhlR protein and, downstream of said first expression cassette, a second expression cassette including a promoter that is induced by the AHL-RhlR complex, said promoter being operably linked to a gene encoding a reporter protein. The invention also includes a genetically modified biosensor cell to detect the presence of microorganisms comprising said gene construct, as well as the method of detecting the presence of microorganisms that produce AHL-type chemical molecules by immobilizing the biosensor in an organic matrix, generating a surface that can be used to expose a sample of a fluid such as air or a liquid medium, and thus detect these molecules mediated by the biosensor cell.