公开(公告)号：US09581536B2

公开(公告)日：2017-02-28

申请号：US14274643

申请日：2014-05-09

申请人： University of Maryland

发明人： Hadar Ben-Yoav ,  Reza Ghodssi ,  Gregory F Payne ,  Deanna L Kelly ,  Eunkyoung Kim ,  Thomas E Winkler

IPC分类号： G01N27/327 ,  G01N15/10 ,  B01L3/00 ,  G01N15/12 ,  G01N15/00

CPC分类号： G01N15/1056 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0887 ,  G01N15/1218 ,  G01N27/3277 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1062 ,  G01N2015/1236 ,  G01N2015/1254

摘要： A lab on a chip device includes a whole blood inlet port and microchannels to transport a whole blood sample or plasma skimmed from the whole blood sample into a detection chamber that includes at least one 3-electrode set of a counter electrode, a working electrode and a reference electrode. The counter electrode, the working electrode and the reference electrode may present bare, unmodified surfaces that are disposed so that clozapine present in the whole blood sample is detected via a reduction-oxidation reaction. Alternatively, the working electrode surface may include catechol grafted to chitosan. A method of detecting analytes and biomarkers includes collecting a whole blood sample, loading the sample into a point-of-care testing (POCT) device that includes at least one working electrode; testing the sample for the occurrence of a redox reaction; and calculating the total oxidative charge when the working electrode is bare or modified as before.

摘要翻译： 芯片装置的实验室包括全血导入口和微通道，用于将全血样品或从全血样品撇去的血浆输送到检测室，该检测室包括至少一个对电极的3电极组，工作电极和 参考电极。 对电极，工作电极和参考电极可以呈现裸露的未改性表面，其被设置为使得通过还原 - 氧化反应检测存在于全血样品中的氯氮平。 或者，工作电极表面可以包括接枝到壳聚糖上的儿茶酚。 检测分析物和生物标志物的方法包括收集全血样品，将样品加载到包括至少一个工作电极的点护理测试（POCT）装置中; 测试样品发生氧化还原反应; 并且如前所述计算工作电极裸露或修改时的总氧化电荷。

公开(公告)号：US09417216B2

公开(公告)日：2016-08-16

申请号：US14157094

申请日：2014-01-16

申请人： University of Maryland

发明人： Young Wook Kim ,  Reza Ghodssi ,  Agisilaos A. Iliadis ,  William E. Bentley ,  Mariana Tsacoumis Meyer

IPC分类号： C12M1/34 ,  G01N29/28 ,  G01N29/02 ,  G01N29/036

CPC分类号： G01N29/28 ,  G01N29/022 ,  G01N29/036 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0423

摘要： A surface acoustic wave (SAW) biofilm sensor includes a transmitting electric to acoustic wave transducer defining an upper surface and a lower surface, a receiving acoustic wave to electric transducer defining an upper surface and a lower surface, a piezoelectric film layer defining an upper surface and a lower surface, and a passivation film layer defining an upper surface and a lower surface. Portions of the lower surface of the piezoelectric film layer are disposed on the upper surface of the transmitting electric to acoustic wave transducer and on the upper surface of the receiving acoustic wave to electric transducer, and the lower surface of the passivation film layer is disposed on the upper surface of the piezoelectric film layer such that the upper surface of the passivation film layer is configured to enable contact with a biofilm.

摘要翻译： 表面声波（SAW）生物膜传感器包括限定上表面和下表面的发射电 - 声波换能器，限定上表面和下表面的电传感器的接收声波，限定上表面和下表面的压电膜层 和下表面，以及限定上表面和下表面的钝化膜层。 将压电薄膜层的下表面的一部分设置在发射电声波换能器的上表面上，并将接收声波的上表面设置在电转换器上，将钝化膜层的下表面设置在 压电膜层的上表面使得钝化膜层的上表面被配置为能够与生物膜接触。

公开(公告)号：US20140332410A1

公开(公告)日：2014-11-13

申请号：US14274643

申请日：2014-05-09

申请人： University of Maryland

发明人： Hadar Ben-Yoav ,  Reza Ghodssi ,  Gregory F. Payne ,  Deanna L Kelly ,  Eunkyoung Kim ,  Thomas E Winkler

IPC分类号： G01N27/327 ,  B01L3/00

CPC分类号： G01N15/1056 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0887 ,  G01N15/1218 ,  G01N27/3277 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1062 ,  G01N2015/1236 ,  G01N2015/1254

摘要： A lab on a chip device includes a whole blood inlet port and microchannels to transport a whole blood sample or plasma skimmed from the whole blood sample into a detection chamber that includes at least one 3-electrode set of a counter electrode, a working electrode and a reference electrode. The counter electrode, the working electrode and the reference electrode may present bare, unmodified surfaces that are disposed so that clozapine present in the whole blood sample is detected via a reduction-oxidation reaction. Alternatively, the working electrode surface may include catechol grafted to chitosan. A method of detecting analytes and biomarkers includes collecting a whole blood sample, loading the sample into a point-of-care testing (POCT) device that includes at least one working electrode; testing the sample for the occurrence of a redox reaction; and calculating the total oxidative charge when the working electrode is bare or modified as before.

摘要翻译： 芯片装置的实验室包括全血导入口和微通道，用于将全血样品或从全血样品撇去的血浆输送到检测室，该检测室包括至少一个对电极的3电极组，工作电极和 参考电极。 对电极，工作电极和参考电极可以呈现裸露的未改性表面，其被设置为使得通过还原 - 氧化反应检测存在于全血样品中的氯氮平。 或者，工作电极表面可以包括接枝到壳聚糖上的儿茶酚。 检测分析物和生物标志物的方法包括收集全血样品，将样品加载到包括至少一个工作电极的点护理测试（POCT）装置中; 测试样品发生氧化还原反应; 并且如前所述计算工作电极裸露或修改时的总氧化电荷。

公开(公告)号：US20140199757A1

公开(公告)日：2014-07-17

申请号：US14157094

申请日：2014-01-16

申请人： University of Maryland

发明人： Young Wook Kim ,  Reza Ghodssi ,  Agisilaos A. Iliadis

IPC分类号： G01N29/28

CPC分类号： G01N29/28 ,  G01N29/022 ,  G01N29/036 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0423

摘要： A surface acoustic wave (SAW) biofilm sensor includes a transmitting electric to acoustic wave transducer defining an upper surface and a lower surface, a receiving acoustic wave to electric transducer defining an upper surface and a lower surface, a piezoelectric film layer defining an upper surface and a lower surface, and a passivation film layer defining an upper surface and a lower surface. Portions of the lower surface of the piezoelectric film layer are disposed on the upper surface of the transmitting electric to acoustic wave transducer and on the upper surface of the receiving acoustic wave to electric transducer, and the lower surface of the passivation film layer is disposed on the upper surface of the piezoelectric film layer such that the upper surface of the passivation film layer is configured to enable contact with a biofilm.

摘要翻译： 表面声波（SAW）生物膜传感器包括限定上表面和下表面的发射电 - 声波换能器，限定上表面和下表面的电传感器的接收声波，限定上表面和下表面的压电膜层 和下表面，以及限定上表面和下表面的钝化膜层。 将压电薄膜层的下表面的一部分设置在发射电声波换能器的上表面上，并将接收声波的上表面设置在电转换器上，将钝化膜层的下表面设置在 压电膜层的上表面使得钝化膜层的上表面被配置为能够与生物膜接触。