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    Membrane-based assays
    1.
    发明申请
    Membrane-based assays 审中-公开

    公开(公告)号：US20080176759A1

    公开(公告)日：2008-07-24

    申请号：US11968078

    申请日：2007-12-31

    申请人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    发明人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    IPC分类号： C40B30/04

    CPC分类号： C40B30/04 ,  C12Q1/18 ,  G01N33/54373 ,  G01N33/554 ,  G01N2500/00

    摘要： Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

    Membrane Based Assays
    2.
    发明申请
    Membrane Based Assays 审中-公开
    基于膜的测定

    公开(公告)号：US20080248492A1

    公开(公告)日：2008-10-09

    申请号：US11968111

    申请日：2007-12-31

    申请人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    发明人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    IPC分类号： G01N33/566 ,  G01N33/68

    CPC分类号： C40B30/04 ,  C12Q1/18 ,  G01N33/54373 ,  G01N33/554 ,  G01N2500/00

    摘要： Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

    摘要翻译： 公开了使用适用于生物传感器的表面检测器阵列器件的基于膜的测定。 该装置由具有限定由一个或多个双层阻挡区隔开的多个不同的双层兼容表面区域的表面的基板形成。 双层相容的表面区域承载它们，由水性膜分离，被支撑的液体双层。 双层可以含有选定的受体或生物分子。 主体水相覆盖基材表面上的双层。 阵列可以被设计成以天然流体双层结构显示天然膜材料，允许高通量发现靶向和影响膜组分的药物。 基于膜的测定通过监测流体双层的一种或多种物理性质中的结合诱导的变化来检测结合事件。

    Membrane-based assays
    3.
    发明授权
    Membrane-based assays 有权
    基于膜的测定

    公开(公告)号：US07407768B2

    公开(公告)日：2008-08-05

    申请号：US10661790

    申请日：2003-09-11

    申请人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    发明人： Victoria Yamazaki ,  Robert J. Schafer ,  Morrison Ulman ,  John T. Groves

    IPC分类号： G01N33/53

    CPC分类号： C40B30/04 ,  C12Q1/18 ,  G01N33/54373 ,  G01N33/554 ,  G01N2500/00

    摘要： Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

    摘要翻译： 公开了使用适用于生物传感器的表面检测器阵列器件的基于膜的测定。 该装置由具有限定由一个或多个双层阻挡区隔开的多个不同的双层兼容表面区域的表面的基板形成。 双层相容的表面区域承载它们，由水性膜分离，被支撑的液体双层。 双层可以含有选定的受体或生物分子。 主体水相覆盖基材表面上的双层。 阵列可以被设计成以天然流体双层结构显示天然膜材料，允许高通量发现靶向和影响膜组分的药物。 基于膜的测定通过监测流体双层的一种或多种物理性质中的结合诱导的变化来检测结合事件。

    Porphyrin catalysts and methods of use thereof
    7.
    发明授权
    Porphyrin catalysts and methods of use thereof 有权
    卟啉催化剂及其使用方法

    公开(公告)号：US08232267B2

    公开(公告)日：2012-07-31

    申请号：US12311640

    申请日：2007-10-05

    申请人： John T. Groves

    发明人： John T. Groves

    IPC分类号： A01N55/02 ,  A01N43/36 ,  A61K31/40 ,  C07B47/00 ,  C07D487/22

    CPC分类号： C07F15/025 ,  C07D487/22 ,  C07F3/003

    摘要： This invention provides a novel class of substituted macrocyclic porphyrin compounds. The compounds are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, or prodrug thereof are also described.

    摘要翻译： 本发明提供了一类新的取代的大环卟啉化合物。 该化合物可用作过氧亚硝酸盐分解催化剂。 还描述了药物组合物，以及制备和使用该化合物或其药学上可接受的盐，水合物或前药的方法。

    Plasmonic System for Detecting Binding of Biological Molecules
    8.
    发明申请
    Plasmonic System for Detecting Binding of Biological Molecules 审中-公开
    用于检测生物分子结合的等离子体系统

    公开(公告)号：US20120208174A1

    公开(公告)日：2012-08-16

    申请号：US13204506

    申请日：2011-08-05

    申请人： William J. Galush ,  Sarah A. Shelby ,  Martin J. Mulvihill ,  Andrea R. Tao ,  Peidong Yang ,  John T. Groves

    发明人： William J. Galush ,  Sarah A. Shelby ,  Martin J. Mulvihill ,  Andrea R. Tao ,  Peidong Yang ,  John T. Groves

    IPC分类号： G01N21/75 ,  G01N21/47 ,  G01N21/59 ,  G01N21/55 ,  B82Y15/00

    CPC分类号： G01N33/54346 ,  G01N21/554

    摘要： Detection and characterization of molecular interactions on membrane surfaces is important to biological and pharmacological research. In one embodiment, silver nanocubes interfaced with glass-supported model membranes form a label-free sensor that measures protein binding to the membrane. The present device and technique utilizes plasmon resonance scattering of nanoparticles, which are chemically coupled to the membrane. In contrast to other plasmonic sensing techniques, this method features simple, solution-based device fabrication and readout. Static and dynamic protein/membrane binding are monitored and quantified.

    摘要翻译： 膜表面分子相互作用的检测和表征对生物学和药理学研究很重要。 在一个实施方案中，与玻璃支持的模型膜相接合的银纳米管形成测量蛋白质结合膜的无标记传感器。 本发明的装置和技术利用等离子体共振散射纳米颗粒，其与化学偶联。 与其他等离子体感测技术相比，该方法具有简单的基于解决方案的器件制造和读出。 静态和动态蛋白/膜结合被监测和定量。

    Fluid Membrane-Based Ligand Display System for Live Cell Assays and Disease Diagnosis Applications
    10.
    发明申请
    Fluid Membrane-Based Ligand Display System for Live Cell Assays and Disease Diagnosis Applications 审中-公开
    用于活细胞分析和疾病诊断应用的流体膜基配体显示系统

    公开(公告)号：US20090011428A1

    公开(公告)日：2009-01-08

    申请号：US12161553

    申请日：2007-01-18

    申请人： Jwa-Min Nam ,  Pradeep M. Nair ,  Richard M. Neve ,  Joe W. Gray ,  John T. Groves

    发明人： Jwa-Min Nam ,  Pradeep M. Nair ,  Richard M. Neve ,  Joe W. Gray ,  John T. Groves

    IPC分类号： G01N33/53

    CPC分类号： G01N33/554

    摘要： A supported membrane based, strategy for the presentation of soluble signaling molecules to living cells is described. In this system, the fluidity of the supported membrane enables localized enrichment of ligand density in a configuration reflecting cognate receptor distribution on the cell surface. Display of a ligand in non-fluid supported membranes produces significantly less cell adhesion and spreading, thus demonstrating that this technique provides a means to control functional soluble ligand exposure in a surface array format. Furthermore, this technique can be applied to tether natively membrane-bound signaling molecules such as ephrin A1 to a supported lipid bilayer. Such a surface can modulate the spreading behavior of metastatic human breast cancer cells displaying ligands and biomolecules of choice. The SLB microenvironment provides a versatile platform that can be tailored to controllably and functionally present a multitude of cell signaling events in a parallel surface array format.

    摘要翻译： 描述了一种用于向活细胞呈递可溶性信号分子的支持膜的策略。 在该系统中，支持的膜的流动性使得能够在反映细胞表面上的同源受体分布的构型中局部富集配体密度。 在非流体支持的膜中显示配体产生显着更少的细胞粘附和扩散，因此证明该技术提供了以表面阵列形式控制功能性可溶性配体暴露的方法。 此外，该技术可以应用于系统地将膜结合的信号分子例如ephrin A1应用于支持的脂质双层。 这种表面可以调节显示配体和选择的生物分子的转移性人乳腺癌细胞的扩散行为。 SLB微环境提供了一个通用平台，可以进行调整，以便可控地和功能地呈现并行表面阵列格式的多个单元信令事件。