公开(公告)号：US20110099648A1

公开(公告)日：2011-04-28

申请号：US12879931

申请日：2010-09-10

申请人： WEI HU ,  ZUOYAN ZHU ,  HONG MA ,  BO GUAN ,  YUANLEI HU ,  ZHONGPING LIN

发明人： WEI HU ,  ZUOYAN ZHU ,  HONG MA ,  BO GUAN ,  YUANLEI HU ,  ZHONGPING LIN

IPC分类号： A01K67/027 ,  C12N15/63 ,  C12N15/00

CPC分类号： C12N15/8509 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/805 ,  C12N2830/85

摘要： The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.

摘要翻译： 本发明公开了一种提高鱼耐受低溶解氧（DO）应力的能力及其用途的重组基因。 使用鲤鱼β-肌动蛋白基因启动子作为启动子，将透明颤菌血红蛋白基因作为靶基因，构建由鲤鱼和肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。 将造型生物斑马鱼作为研究对象，将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后，获得转基因鱼的成活率为92％，与对照鱼组65％的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。 当将重组基因应用于经济养殖的物种，即钝鼻鲷（Megalobrama amblycephala）和鲤鱼（Cyprinus carpio L.）时，也增强了它们的缺氧耐受性。 这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。