公开(公告)号：US20240219275A1

公开(公告)日：2024-07-04

申请号：US18604005

申请日：2024-03-13

申请人： Waters Technologies Corporation

发明人： Paul Rainville ,  Kerri Smith

IPC分类号： G01N1/40 ,  G01N30/02 ,  G01N30/72 ,  G01N30/86

CPC分类号： G01N1/405 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

摘要： The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20230081572A1

公开(公告)日：2023-03-16

申请号：US17990029

申请日：2022-11-18

申请人： Waters Technologies Corporation

发明人： Paul Rainville ,  Kerri Smith

IPC分类号： G01N1/40 ,  G01N30/72 ,  G01N30/86 ,  G01N30/02

摘要： The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20220205961A1

公开(公告)日：2022-06-30

申请号：US17564753

申请日：2021-12-29

申请人： Waters Technologies Corporation

发明人： Jinchuan Yang ,  Paul Rainville

IPC分类号： G01N30/72 ,  G01N33/15 ,  G01N30/60 ,  H01J49/42

摘要： The present disclosure discusses a method of separating a sample (e.g., B vitamins and their vitamers) including coating a flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating (e.g., organosilica coating) of the flow path. The applied coating provides an inert barrier that is beneficial for B vitamin analysis, including improved peak shape (less tailing and narrower peak), high sensitivity, and no carry-over.

公开(公告)号：US12117427B2

公开(公告)日：2024-10-15

申请号：US18216064

申请日：2023-06-29

申请人： Waters Technologies Corporation

发明人： Kerri M. Smith ,  Paul Rainville

IPC分类号： G01N30/72 ,  C23C14/04 ,  C23C14/12 ,  C23C14/46 ,  G01N30/02

CPC分类号： G01N30/72 ,  C23C14/046 ,  C23C14/12 ,  G01N2030/027

摘要： The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

公开(公告)号：US11740211B2

公开(公告)日：2023-08-29

申请号：US17150802

申请日：2021-01-15

申请人： Waters Technologies Corporation

发明人： Kerri M. Smith ,  Paul Rainville

IPC分类号： G01N30/72 ,  C23C14/04 ,  C23C14/12 ,  G01N30/02

CPC分类号： G01N30/72 ,  C23C14/046 ,  C23C14/12 ,  G01N2030/027

摘要： The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

公开(公告)号：US20200096525A1

公开(公告)日：2020-03-26

申请号：US16575909

申请日：2019-09-19

申请人： Waters Technologies Corporation

发明人： Lee Gethings ,  Giorgis M. Isaac ,  Nyasha Clarence Munjoma ,  Robert S. Plumb ,  Paul Rainville

IPC分类号： G01N33/92 ,  G01N30/02 ,  B01D15/30

摘要： The present disclosure relates to methodologies, systems, and devices for screening lipids. The technique includes selecting a set of standards to identify at least one desired class of lipids, spiking the standards into a biological sample to form a sample matrix, extracting the lipids from the sample matrix, and introducing the sample matrix into a chromatography system to separate the desired class of lipids. Once the lipids are separated into different lipid classes using HILIC chromatography, they are directed to a detector, and the separated lipids are quantified based on a comparison between the measured detector response and a calibration curve generated with a known concentration of the selected set of standards.

公开(公告)号：US20190339238A1

公开(公告)日：2019-11-07

申请号：US16400163

申请日：2019-05-01

申请人： Waters Technologies Corporation

发明人： Jinchuan Yang ,  Paul Rainville

IPC分类号： G01N30/86 ,  G01N30/72 ,  G01N33/00 ,  G01N30/74

摘要： A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.

公开(公告)号：US11959842B2

公开(公告)日：2024-04-16

申请号：US17990029

申请日：2022-11-18

申请人： Waters Technologies Corporation

发明人： Paul Rainville ,  Kerri Smith

IPC分类号： G01N30/72 ,  G01N1/40 ,  G01N30/86 ,  G01N30/02

CPC分类号： G01N1/405 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

摘要： The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20230256413A1

公开(公告)日：2023-08-17

申请号：US18138991

申请日：2023-04-25

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Paul Rainville ,  Jacob N. Fairchild ,  Babjide Okandeji ,  Nicole L. Lawrence ,  Dimple Shah

IPC分类号： B01J20/288 ,  B01D15/32 ,  B01J20/287 ,  B01D15/36 ,  B01D15/38 ,  B01J20/28 ,  B01J20/283

CPC分类号： B01J20/288 ,  B01D15/327 ,  B01J20/287 ,  B01D15/36 ,  B01D15/3847 ,  B01J20/28061 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/283 ,  B01J2220/54 ,  B01J2220/80

摘要： The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention, separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, α-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

公开(公告)号：US11679341B2

公开(公告)日：2023-06-20

申请号：US17244451

申请日：2021-04-29

申请人： Waters Technologies Corporation

发明人： Jinchuan Yang ,  Paul Rainville

IPC分类号： B01D15/38 ,  B01D15/40

CPC分类号： B01D15/3833 ,  B01D15/40

摘要： The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-α-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of α-tocopherol as natural or synthetic.