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    Optimization of pest resistance genes using DNA shuffling
    1.
    发明授权
    Optimization of pest resistance genes using DNA shuffling 有权
    使用DNA改组优化害虫抗性基因

    公开(公告)号：US06500617B1

    公开(公告)日：2002-12-31

    申请号：US09296886

    申请日：1999-04-22

    申请人： Willem P. C. Stemmer ,  Linda A. Castle ,  Takashi Yamamoto

    发明人： Willem P. C. Stemmer ,  Linda A. Castle ,  Takashi Yamamoto

    IPC分类号： C12Q168

    CPC分类号： C12N15/8285 ,  C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

    摘要： This invention provides methods of obtaining pest resistance genes that are improved over naturally occurring genes for use in conferring upon plants resistance to pests. The methods involve the use of DNA shuffling of pest resistance genes to produce libraries of recombinant pest resistance genes, which are then screened to identify those that exhibit the improved property or properties of interest.

    摘要翻译： 本发明提供了获得害虫抗性基因的方法，该基因比用于赋予植物抗病虫害性的天然存在的基因更好。 该方法涉及使用有害动物抗性基因的DNA改组来产生重组害虫抗性基因的文库，然后对其进行筛选以鉴定那些表现出改善的感兴趣的性质或性质的基因。

    CRYSTAL STRUCTURE OF GLYPHOSATE ACETYLTRANSFERASE (GLYAT) AND METHODS OF USE
    6.
    发明申请
    CRYSTAL STRUCTURE OF GLYPHOSATE ACETYLTRANSFERASE (GLYAT) AND METHODS OF USE 审中-公开
    谷氨酸乙酰胆碱酯酶（GLYAT）的晶体结构及其使用方法

    公开(公告)号：US20120288914A1

    公开(公告)日：2012-11-15

    申请号：US13382780

    申请日：2010-07-07

    申请人： Daniel Siehl ,  Robert J. Keenan ,  Zhenglin Hou ,  Linda A. Castle

    发明人： Daniel Siehl ,  Robert J. Keenan ,  Zhenglin Hou ,  Linda A. Castle

    IPC分类号： C12N9/10 ,  G06F19/16

    CPC分类号： C12N9/1029 ,  C07K2299/00

    摘要： The presently disclosed subject matter provides compositions and methods for evaluating the potential of candidate polypeptides to associate with glyphosate with a higher binding affinity, higher binding specificity, or both or to have N-acetyltransferase activity with a higher catalytic rate when compared to a native glyphosate acetyltransferase (GLYAT) polypeptide through the provision and comparison of three-dimensional molecular structures of the candidate polypeptides and the GLYAT polypeptides provided herein. The methods further provide for identification of polypeptides with these advantageous properties using the three-dimensional molecular structures of GLYAT polypeptides.

    摘要翻译： 目前公开的主题提供了组合物和方法，用于与天然草甘膦相比，评估候选多肽与具有更高结合亲和力，更高结合特异性或两者的草甘膦缔合或具有较高催化速率的N-乙酰转移酶活性的潜在性 乙酰转移酶（GLYAT）多肽，通过提供和比较本文提供的候选多肽和GLYAT多肽的三维分子结构。 所述方法进一步提供了使用GLYAT多肽的三维分子结构鉴定具有这些有利性质的多肽。

    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast
    7.
    发明申请
    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
    用于靶向叶绿体感兴趣的方法和组合物

    公开(公告)号：US20120042412A1

    公开(公告)日：2012-02-16

    申请号：US13208960

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： A01H1/06 ,  C12N15/82 ,  C12N5/10 ,  C12N9/96 ,  A01H5/10 ,  C07K19/00 ,  C12N9/02 ,  C12N15/62 ,  A01H5/00

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。