Optimization of pest resistance genes using DNA shuffling
    1.
    发明授权
    Optimization of pest resistance genes using DNA shuffling 有权
    使用DNA改组优化害虫抗性基因

    公开(公告)号:US06500617B1

    公开(公告)日:2002-12-31

    申请号:US09296886

    申请日:1999-04-22

    IPC分类号: C12Q168

    摘要: This invention provides methods of obtaining pest resistance genes that are improved over naturally occurring genes for use in conferring upon plants resistance to pests. The methods involve the use of DNA shuffling of pest resistance genes to produce libraries of recombinant pest resistance genes, which are then screened to identify those that exhibit the improved property or properties of interest.

    摘要翻译: 本发明提供了获得害虫抗性基因的方法,该基因比用于赋予植物抗病虫害性的天然存在的基因更好。 该方法涉及使用有害动物抗性基因的DNA改组来产生重组害虫抗性基因的文库,然后对其进行筛选以鉴定那些表现出改善的感兴趣的性质或性质的基因。

    Methods for identifying and producing polypeptides
    6.
    发明授权
    Methods for identifying and producing polypeptides 有权
    用于鉴定和产生多肽的方法

    公开(公告)号:US07957912B2

    公开(公告)日:2011-06-07

    申请号:US12557463

    申请日:2009-09-10

    摘要: In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.

    摘要翻译: 在硅胶核酸重组方法中,提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。 一种公开的方法涉及鉴定和产生多肽。 该方法可以包括(a)接收识别编码多肽的两个或多个亲本多肽或亲本核酸的序列的数据; (b)在序列上选择一个或多个交叉位点,从而限定一个或多个重组多肽或重组核酸; (c)选择至少一种重组多肽或重组核酸; (d)重组一种或多种寡核苷酸,其中至少一种具有与(c)中选择的重组多肽或重组核酸的部分或全部相匹配的序列; (e)选择(d)中产生的至少一些重组寡核苷酸; 和(f)产生由所选择的核酸编码的多肽。