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公开(公告)号：US07695900B2
公开(公告)日：2010-04-13
申请号：US11711560
申请日：2007-02-27
CPC分类号： C12Q1/025 , C12Q1/04 , Y10S435/975
摘要： This application claims a method for detecting biologically active substances, comprising the following steps a) providing a support carrying substances to be tested b) providing a suspension containing luminescent microorganisms, c) coating the support with the suspension of microorganisms, d) detecting the biologically active substances on the support by detecting the change in luminescence of the suspension of microorganisms, and e) stimulating the luminescence of the microorganisms before or during detection, and/or f) extending the period of luminescence of the microorganisms by employing substances for regulating and extending the period of luminescence of the microorganisms.
摘要翻译： 本申请要求一种用于检测生物活性物质的方法，包括以下步骤：a）提供携带待测物质的载体b）提供含有发光微生物的悬浮液，c）用微生物悬浮液涂覆载体，d） 通过检测微生物悬浮液的发光变化，并且e）在检测前或检测期间刺激微生物的发光，和/或f）通过使用用于调节和调节微生物的物质来延长微生物的发光期 延长微生物的发光时间。
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公开(公告)号：US07183113B2
公开(公告)日：2007-02-27
申请号：US10424067
申请日：2003-04-25
IPC分类号： G01N33/53
CPC分类号： C12Q1/025 , C12Q1/04 , Y10S435/975
摘要： The invention claimed herein is a kit for detecting biologically active substances on a support, comprising the luminescent marine bacteria Vibria Fischeri in a form stabilized for transport and storage, one or more cultivation media or the individual components of cultivation media for the microorganism wherein the cultivation media contains 100 mg/l to 500 mg/l of aspartic acid, and optionally additives for extending the period of luminescence selected from N-acylhomoserine lactones, dipeptides, oligopeptides, or their biochemical precursors, and boron compounds.
摘要翻译： 本文所要求的发明是用于检测载体上的生物活性物质的试剂盒，其包含以稳定运输和储存的形式的发光海洋细菌Vibria Fischeri，一种或多种培养基或微生物培养基的各组分，其中培养 培养基含有100mg / l至500mg / l的天冬氨酸，以及任选的用于延长选自N-酰基高丝氨酸内酯，二肽，寡肽或其生化前体的发光期和硼化合物的添加剂。
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