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    High affinity ligands of basic fibroblast growth factor and thrombin
    4.
    发明授权
    High affinity ligands of basic fibroblast growth factor and thrombin 失效
    碱性成纤维细胞生长因子和凝血酶的高亲和力配体

    公开(公告)号：US06177557B1

    公开(公告)日：2001-01-23

    申请号：US08687421

    申请日：1996-08-05

    申请人： Nebojsa Janjic ,  Larry Gold ,  Diane Tasset

    发明人： Nebojsa Janjic ,  Larry Gold ,  Diane Tasset

    IPC分类号： C07H2102

    CPC分类号： C12N15/115 ,  C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/6811 ,  C40B40/00

    摘要： The present invention utilizes the SELEX (Systematic Evolution of Ligands for EXponential Enrichment) method for identifying and preparing nucleic acid ligands to basic fibroblast growth factor (bFGF) and thrombin. Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and 2′-amino-modified RNA ligands to bFGF. Further included in the present invention are modified nucleotide sequences to thrombin based on the sequences of the RNA ligands identified. The modified RNA ligands to bFGF and thrombin exhibit increased in vivo stability.

    摘要翻译： 本发明利用SELEX（用于指数浓缩的配体的系统演化）方法来鉴定和制备碱性成纤维细胞生长因子（bFGF）和凝血酶的核酸配体。 本发明中包括bFGF的核酸配体，其是bFGF的抑制剂和bFGF的2'-氨基修饰的RNA配体。 进一步包括在本发明中的是基于所鉴定的RNA配体的序列的凝血酶的修饰的核苷酸序列。 bFGF和凝血酶的修饰的RNA配体表现出增加的体内稳定性。

    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands
    7.
    发明申请
    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands 有权
    通过与核酸配体竞争来测定与核酸分子结合的非核酸分子

    公开(公告)号：US20050048521A1

    公开(公告)日：2005-03-03

    申请号：US10746565

    申请日：2003-12-24

    申请人： Nebojsa Janjic ,  Larry Gold

    发明人： Nebojsa Janjic ,  Larry Gold

    IPC分类号： G01N33/50 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G01N33/543

    CPC分类号： G01N33/53 ,  C12Q1/6811 ,  Y10S530/812 ,  C12Q2545/107 ,  C12Q2565/518

    摘要： A competitive binding assay is used to determine whether a non-nucleic acid molecule from a library of non-nucleic acid molecules binds to a target. The non-nucleic acid molecule competes with a labeled nucleic acid ligand for binding to the target which may be immobilized. Detecting displacement of labeled nucleic acid ligand from a complex of the labeled nucleic acid ligand and the target determines binding of the non-nucleic acid molecule to the target. The nucleic acid ligand may be immobilized and contacted with a labeled target to form a complex. Adding a non-nucleic acid molecule to the complex displaces labeled target from the complex, and detecting displacement of the labeled target determines binding of the non-nucleic acid molecule to the target. Labeled nucleic acid ligand or labeled target displaced from or remaining in the complex can be detected for detecting displacement. Nucleic acid ligands that bind to the target are identified by the SELEX method. The assay provides a high throughput screening method for determining whether a non-nucleic acid molecule binds to a target.

    摘要翻译： 使用竞争性结合测定来确定非核酸分子的文库中的非核酸分子是否与靶标结合。 非核酸分子与标记的核酸配体竞争结合靶，其可以被固定化。 检测标记的核酸配体从标记的核酸配体和靶的复合物的置换确定非核酸分子与靶标的结合。 核酸配体可以被固定化并与标记的靶接触以形成复合物。 向复合物中加入非核酸分子从复合物置换标记的靶，并且检测标记的靶的置换确定非核酸分子与靶的结合。 可以检测标记的核酸配体或从复合物中残留的残留的标记的靶标，以检测位移。 通过SELEX法鉴定与靶标结合的核酸配体。 该测定提供了用于确定非核酸分子是否结合靶的高通量筛选方法。

    Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
    9.
    发明授权
    Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes 有权
    血管内皮生长因子（VEGF）核酸配体复合物

    公开(公告)号：US06426335B1

    公开(公告)日：2002-07-30

    申请号：US09254968

    申请日：2000-03-13

    申请人： Nebojsa Janjic ,  Larry Gold ,  Paul Schmidt ,  Chandra Vargeese ,  Michael Willis

    发明人： Nebojsa Janjic ,  Larry Gold ,  Paul Schmidt ,  Chandra Vargeese ,  Michael Willis

    IPC分类号： A61K3170

    CPC分类号： A61K31/70 ,  A61K38/00 ,  A61K47/544 ,  A61K47/60 ,  C07H21/00 ,  C07K14/52 ,  C12N15/115 ,  C12N15/88 ,  C12N2310/3515

    摘要： This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound by identifying a VEGF Nucleic Acid Ligand by SELEX methodology and associating the VEGF Nucleic Acid Ligand with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further discloses Complexes comprising one or more VEGF Nucleic Acid Ligands in association with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further includes a Lipid construct comprising a VEGF Nucleic Acid Ligand or Complex and methods for making the same.

    摘要翻译： 本发明公开了一种通过SELEX方法鉴定VEGF核酸配体并将VEGF核酸配体与非 - 免疫原性，高分子量化合物或非亲和性化合物组合的复合物， 免疫原性，高分子量化合物或亲脂性化合物。 本发明进一步公开了包含与非免疫原性，高分子量化合物或亲脂性化合物结合的一种或多种VEGF核酸配体的复合物。 本发明还包括含有VEGF核酸配体或复合物的脂质构建体及其制备方法。