公开(公告)号：US20070199105A1

公开(公告)日：2007-08-23

申请号：US11614098

申请日：2006-12-21

申请人： Xiaoming Bao ,  George Singletary ,  Deborah Wetterberg ,  Ramesh Nair ,  Kanwarpal Dhugga ,  Matthias Liebergesell ,  David Selinger

发明人： Xiaoming Bao ,  George Singletary ,  Deborah Wetterberg ,  Ramesh Nair ,  Kanwarpal Dhugga ,  Matthias Liebergesell ,  David Selinger

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/88 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/8261 ,  C12Y401/01035 ,  Y02A40/146

摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.

摘要翻译： 本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。 在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。 这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。 这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（UXS）基因表达的一种或多种核酸序列。

公开(公告)号：US20070089200A1

公开(公告)日：2007-04-19

申请号：US11512956

申请日：2006-08-29

申请人： Xiaoming Bao ,  Darren Gruis ,  Craig Hastings ,  George Singletary ,  Deborah Wetterberg

发明人： Xiaoming Bao ,  Darren Gruis ,  Craig Hastings ,  George Singletary ,  Deborah Wetterberg

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H1/00

CPC分类号： C12N15/8245 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

摘要： Compositions and methods for altering the levels of reversibly glycosylated polypeptide (RGP) in plants are provided. The present invention recognizes that by altering the level of expression of RGP, plants and seeds having beneficial qualities can be obtained. The invention involves controlled suppression of RGP expression to obtain plants and seeds having decreased amounts of hemicellulose and arabinose. Controlled suppression of RGP, particularly in vegetative tissues and seeds, leads to plants with desirable characteristics, including altered cell wall structure, reduced grain fiber content, increased seed protein, increased oil content, and altered arabinoxylan or xyloglucan levels. Compositions of the invention include constructs for the suppression of RGP and transformed plants and seeds having the altered phenotype of the invention.

摘要翻译： 提供了用于改变植物中可逆糖基化多肽（RGP）水平的组合物和方法。 本发明认识到，通过改变RGP的表达水平，可以获得具有有益品质的植物和种子。 本发明涉及RGP表达的受控抑制以获得具有减少量的半纤维素和阿拉伯糖的植物和种子。 RGP的受控抑制，特别是在营养组织和种子中，导致具有所需特征的植物，包括改变的细胞壁结构，减少的谷物纤维含量，增加的种子蛋白，增加的油含量和改变的阿糖木聚糖或木葡聚糖水平。 本发明的组合物包括用于抑制RGP的转化植物和具有改变的本发明表型的转化植物和种子的构建体。

公开(公告)号：US08030540B2

公开(公告)日：2011-10-04

申请号：US10907936

申请日：2005-04-21

申请人： Hanping Guan ,  Deborah Wetterberg ,  Angela L. McKean ,  Peter L. Keeling

发明人： Hanping Guan ,  Deborah Wetterberg ,  Angela L. McKean ,  Peter L. Keeling

IPC分类号： C12N15/82 ,  C12N15/63 ,  C12N15/31 ,  A01H5/10 ,  A01H5/00 ,  A01H3/00

CPC分类号： C12N15/8247 ,  C12N9/1241 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

摘要： The invention provides transgenic corn seed, which expresses a gene encoding a double mutant of the E. coli glgC gene in endosperm plastids, wherein the mutant protein has a proline to aspartic acid substitution at amino acid 295 and a glutamic acid to lysine substitution at amino acid 296. The transgenic corn seed of the invention is characterized by enhanced levels of a number of amino acids and oil, when compared to isogenic corn seed, which does not express the transgene in an endosperm plastid. However, the amount of starch in the transgenic corn seed of the invention is decreased or unchanged when compared to the amount of starch in the isogenic control corn seed.

摘要翻译： 本发明提供了转基因玉米种子，其表达编码胚乳质体中大肠杆菌glgC基因的双重突变体的基因，其中突变蛋白质具有在氨基酸295处天冬氨酸取代的脯氨酸，并且在氨基酸处具有谷氨酸至赖氨酸取代 酸296.当与在胚乳质体中不表达转基因的等基因玉米种子相比，本发明的转基因玉米种子的特征在于增加多种氨基酸和油的水平。 然而，与同基因对照玉米种子中的淀粉的量相比，本发明的转基因玉米种子中的淀粉的量减少或不变。