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公开(公告)号:US06649390B1
公开(公告)日:2003-11-18
申请号:US09530851
申请日:2000-05-04
申请人: Xiaoyu Sheng , Yumin Mao , Youzhong Yuan , Chaoneng Ji , Zongxiang Zhou
发明人: Xiaoyu Sheng , Yumin Mao , Youzhong Yuan , Chaoneng Ji , Zongxiang Zhou
IPC分类号: C12N916
CPC分类号: C12N9/16
摘要: The invention belongs to the field of biological engineering techniques and relates to a thermophilic alkaline phosphoesterase. The invention provides an amino acid sequence of a thermophilic alkaline phosphoesterase of the invention, as well as a DNA fragment encoding the amino acid sequence. The present invention also includes methods for cloning an expression vector containing the DNA fragment or a portion thereof and for producing the recombinant enzyme. The invention also relates to a method for tagging biological macromolecules utilizing the enzyme.
摘要翻译: 本发明属于生物工程技术领域,涉及嗜热碱性磷酸酯酶。 本发明提供本发明的嗜热碱性磷酸酯酶的氨基酸序列,以及编码氨基酸序列的DNA片段。 本发明还包括用于克隆含有DNA片段或其部分并用于产生重组酶的表达载体的方法。 本发明还涉及一种利用该酶标记生物大分子的方法。