-
公开(公告)号:US20080064866A1
公开(公告)日:2008-03-13
申请号:US11856978
申请日:2007-09-18
申请人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
IPC分类号: C07H21/04
CPC分类号: C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
-
2.
公开(公告)号:US20070266456A1
公开(公告)日:2007-11-15
申请号:US11747824
申请日:2007-05-11
申请人: Xudong Ye , Michael Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul Chomet
发明人: Xudong Ye , Michael Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul Chomet
CPC分类号: C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
摘要: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
摘要翻译: 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。
-
公开(公告)号:US20070074314A1
公开(公告)日:2007-03-29
申请号:US11516036
申请日:2006-09-05
申请人: Xudong Ye , Larry Gilbertson , Michael Petersen
发明人: Xudong Ye , Larry Gilbertson , Michael Petersen
CPC分类号: C12N15/8205
摘要: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
摘要翻译: 提供了用于改善细菌介导的植物转化的方法和组合物。 这些方法通常允许具有减少的载体骨架整合和高频率的低拷贝转化事件的植物转化。 描述了用于实现这些结果的载体,以及它们使用的方法。
-
公开(公告)号:US20080066202A1
公开(公告)日:2008-03-13
申请号:US11856958
申请日:2007-09-18
申请人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
CPC分类号: C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译: 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因,阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体,将植物细胞再生成植物并鉴定独立的转基因基因座,其中可选择标记基因或转基因元件可以在后代中分离。
-
公开(公告)号:US20080070296A1
公开(公告)日:2008-03-20
申请号:US11857000
申请日:2007-09-18
申请人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
发明人: Toni Armstrong , Kenneth Barton , Laura Crow , Larry Gilbertson , Shihshieh Huang , Yong Huang , Brian Martinell , Michael Petersen , Kenneth Piller , Qi Wang , Xudong Ye
IPC分类号: C12N15/00
CPC分类号: C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要翻译: 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因,阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体,将植物细胞再生成植物并鉴定独立的转基因基因座,其中可选择标记基因或转基因元件可以在后代中分离。
-
公开(公告)号:US08466345B2
公开(公告)日:2013-06-18
申请号:US13213021
申请日:2011-08-18
申请人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
发明人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC分类号: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
摘要: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
摘要翻译: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆,玉米,棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。
-
公开(公告)号:US08357834B2
公开(公告)日:2013-01-22
申请号:US13213015
申请日:2011-08-18
申请人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
发明人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC分类号: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
摘要: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
-
公开(公告)号:US08030544B2
公开(公告)日:2011-10-04
申请号:US12045562
申请日:2008-03-10
申请人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
发明人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC分类号: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
摘要: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
摘要翻译: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆,玉米,棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。
-
公开(公告)号:US20090138985A1
公开(公告)日:2009-05-28
申请号:US12045562
申请日:2008-03-10
申请人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
发明人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC分类号: C12N15/11
CPC分类号: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
摘要: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
摘要翻译: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆,玉米,棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。
-
公开(公告)号:US20120077264A1
公开(公告)日:2012-03-29
申请号:US13213021
申请日:2011-08-18
申请人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
发明人: Brian Martinell , Michael Petersen , David Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC分类号: C12N15/82
CPC分类号: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
摘要: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
摘要翻译: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆,玉米,棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。
-
-
-
-
-
-
-
-
-