Decoration light
    3.
    外观设计

    公开(公告)号:USD985153S1

    公开(公告)日:2023-05-02

    申请号:US29770167

    申请日:2021-02-10

    申请人: Yan Wang

    设计人: Yan Wang

    Method for correcting interference of multicolor fluorescence channels

    公开(公告)号:US11579086B2

    公开(公告)日:2023-02-14

    申请号:US17496262

    申请日:2021-10-07

    IPC分类号: C12Q1/686 G01N21/64 B01L9/00

    摘要: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.

    Method for Analyzing digital PCR Data Using Multicolor Fluorescence Reader

    公开(公告)号:US20220026363A1

    公开(公告)日:2022-01-27

    申请号:US17496285

    申请日:2021-10-07

    IPC分类号: G01N21/64 B01L9/00 C12Q1/686

    摘要: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.

    Method for Correcting Interference of Multicolor Fluorescence Channels

    公开(公告)号:US20220026362A1

    公开(公告)日:2022-01-27

    申请号:US17496262

    申请日:2021-10-07

    IPC分类号: G01N21/64 B01L9/00 C12Q1/686

    摘要: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.

    Chair
    7.
    外观设计
    Chair 有权

    公开(公告)号:USD933383S1

    公开(公告)日:2021-10-19

    申请号:US29780385

    申请日:2021-04-23

    申请人: Yan Wang

    设计人: Yan Wang

    Wireless receiving circuit and wireless control device

    公开(公告)号:US10993305B2

    公开(公告)日:2021-04-27

    申请号:US16722070

    申请日:2019-12-20

    申请人: Yan Wang

    发明人: Yan Wang

    IPC分类号: H05B47/19 H04W4/00 H04W4/80

    摘要: A wireless receiving circuit and a wireless control device. The wireless receiving circuit includes a signal receiving module, a Bluetooth signal processing module, a voltage stabilization module, and an output control module. An output end of the signal receiving module is connected with an input end of the Bluetooth signal processing module, and a voltage end of the Bluetooth signal processing module is connected with an output end of the voltage stabilization module. An output end of the Bluetooth signal processing module is connected with an input end of the output control module. The signal receiving module is to receive an external Bluetooth signal and is to transmit the signal to the Bluetooth signal processing module. The Bluetooth signal processing module is to process the external Bluetooth signal and is to transmit a control signal to the output control module.

    Method for Detecting multiple DNA Mutations and Copy Number Variations

    公开(公告)号:US20200277654A1

    公开(公告)日:2020-09-03

    申请号:US16875381

    申请日:2020-05-15

    申请人: Yan Wang

    发明人: Yan Wang

    摘要: Disclosed are methods for detecting DNA mutations of target genes in a DNA sample by combining single-molecule clonal amplification and mutant primer specific extension detection. In the method, thousands and millions of DNA molecules are locally amplified to form immobilized DNA clusters of identical sequences. Mutation specific primers are used to anneal to the mutant sequences in the DNA clusters and are extended by DNA polymerase to make labeled DNA strands. The labeled DNA clusters are detected to identify the DNA clusters of mutant sequences. This method enables detection of single mutation molecule and direct enumeration of mutation molecules in the sample. Once generated from a DNA sample, the immobilized DNA clusters can be reused many times for detection of different mutations or sequences of interest. Methods for determining differential gene expression and chromosome copy number variation are also disclosed.

    Method for uses of proinsulin transferrin fusion proteins as prodrugs

    公开(公告)号:US10513563B2

    公开(公告)日:2019-12-24

    申请号:US13476812

    申请日:2012-05-21

    摘要: The present invention provides compositions useful as prodrugs and methods for making the same. The compositions include a fusion protein having a first delivery domain and a second protein precursor domain linked together via a linker sequence. The delivery domain is a protein capable of facilitating entry to a target cells via the endocytotic pathway, such as transferrin. The protein precursor is a prohormone or a profactor, such as proinsulin. Methods of this invention include the steps of selecting a protein suitable as the delivery domain, constructing a vector to encode the fusion protein, and expressing the fusion protein in a suitable expression host. Also disclosed is a method for targeted-delivery of prodrugs to livers and a method of reducing hepatic glucose production.