DRAM with reduced electric power consumption
    3.
    发明授权
    DRAM with reduced electric power consumption 有权
    DRAM具有降低的电力消耗

    公开(公告)号:US6097658A

    公开(公告)日:2000-08-01

    申请号:US189148

    申请日:1998-11-10

    摘要: A DRAM (Dynamic Random Access Memory) having a plurality of memory cells includes a data read/write circuit reading or writing data for the memory cells, a self-refresh circuit refreshing data stored in the memory cells, and a power supply unit for supplying electric power to the data read/write circuit and the self-refresh circuit, the electric power having a first voltage level in a normal operation mode and a second voltage level in a self-refresh mode, wherein the second voltage level is lower than the first voltage level.

    摘要翻译: 具有多个存储单元的DRAM(动态随机存取存储器)包括数据读/写电路读取或写入存储单元的数据,自刷新电路刷新存储在存储单元中的数据,以及电源单元, 数据读/写电路和自刷新电路的电力,电力在正常操作模式下具有第一电压电平,在自刷新模式下具有第二电压电平,其中第二电压电平低于 第一电压电平。

    METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM
    5.
    发明申请
    METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
    使用重组微生物生产聚酯共聚物的方法

    公开(公告)号:US20110104768A1

    公开(公告)日:2011-05-05

    申请号:US12989333

    申请日:2009-04-23

    IPC分类号: C12P7/62 C12N1/00 C12N1/21

    CPC分类号: C12P7/62

    摘要: The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

    摘要翻译: 本发明的目的是提供一种利用糖作为原料,通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。 提供以下:由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法,其特征在于,包括以下步骤:(1)培养具有能够催化将CoA转移到丙酸和/或 乳酸盐,能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质,能够催化乙酰乙酰辅酶A还原反应的蛋白质,以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质 含有碳源的培养基中的酸序列(a)或(b):(a)从SEQ ID NO:2所示的氨基酸序列衍生的氨基酸序列,通过将位置130的至少一个氨基酸置换 ,325,477和481具有不同的氨基酸; 或(b)衍生自(a)中规定的蛋白质的氨基酸序列,通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个 氨基酸残基; 和(2)从步骤(1)获得的培养产物中收集聚酯共聚物。 根据本发明的制造方法,可以使用廉价的碳源作为原料,有效地制造由3HB和LA组成的聚酯共聚物,从而可以降低生物降解性塑料的制造成本。

    Method for production of polyester copolymer using recombinant microorganism
    6.
    发明授权
    Method for production of polyester copolymer using recombinant microorganism 有权
    使用重组微生物生产聚酯共聚物的方法

    公开(公告)号:US08563281B2

    公开(公告)日:2013-10-22

    申请号:US12989333

    申请日:2009-04-23

    IPC分类号: C12P7/62 C12N1/20 C12N1/00

    CPC分类号: C12P7/62

    摘要: The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

    摘要翻译: 本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。 该方法可以包括培养表达:能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。 根据该制造方法,可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物,从而可以降低生物降解性塑料的制造成本。