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    Anti-HIV monoclonal antibody
    1.
    发明授权
    Anti-HIV monoclonal antibody 失效
    抗HIV单克隆抗体

    公开(公告)号：US6114143A

    公开(公告)日：2000-09-05

    申请号：US513968

    申请日：1995-09-11

    申请人： Yasuyuki Eda ,  Hiroaki Maeda ,  Keiichi Makizumi ,  Kouichi Shiosaki ,  Kiyoshi Osatomi ,  Kazuhiko Kimachi ,  Hirofumi Higuchi ,  Sachio Tokiyoshi

    发明人： Yasuyuki Eda ,  Hiroaki Maeda ,  Keiichi Makizumi ,  Kouichi Shiosaki ,  Kiyoshi Osatomi ,  Kazuhiko Kimachi ,  Hirofumi Higuchi ,  Sachio Tokiyoshi

    IPC分类号： A61K38/00 ,  C07K16/10 ,  C12P21/04 ,  C12P21/08 ,  C12N15/09 ,  A61K39/395 ,  C12P11/00

    CPC分类号： C07K16/1063 ,  A61K38/00 ,  C07K2317/24

    摘要： A monoclonal antibody useful for clinical application which recognizes the conserved region of V3-PND region of glycoprotein antigen having a molecular weight of about 1.2.times.10.sup.5 daltons (gp120) on a coating membrane of human immunodeficiency virus (HIV) and which has an ability to neutralize a broad range of various HIV variants, or a fragment thereof, and the chimeric and humanized antibodies derived therefrom are provided. By using as an immunogen a plurality of peptides having PND-Tip region containing the highly conserved GPGR sequence within PND of HIV gp120, a monoclonal antibody having a neutralizing activity to many HIV variants can be prepared. By transplanting the gene fragment coding for the variable region of said monoclonal antibody or complementarity determining region (CDR) of said region to a human antibody gene, a chimeric antibody or a reshaped antibody having an anti-HIV neutralizing activity which are effective for clinical application can be obtained.

    摘要翻译： PCT No.PCT / JP94 / 00371 Sec。 371 1995年9月11日第 102（e）1995年9月11日PCT PCT 1994年3月9日PCT公布。 公开号WO94 / 20632 日期1994年9月15日一种可用于临床应用的单克隆抗体，其识别在人免疫缺陷病毒（HIV）的包被膜上具有约1.2×10 5道尔顿（gp120）分子量的糖蛋白抗原的V3-PND区的保守区， 具有中和各种各样的HIV变体或其片段的能力，并且提供了由其衍生的嵌合和人源化抗体。 通过使用多种具有含有HIV gp120的PND内的高度保守的GPGR序列的PND-Tip区的肽，可以制备对许多HIV变体具有中和活性的单克隆抗体。 通过将编码所述区域的所述单克隆抗体或互补决定区（CDR）的可变区的基因片段移植到人抗体基因，嵌合抗体或具有抗HIV中和活性的重构抗体，其对临床应用有效 可以获得。

    Recombinant anti-HIV antibody and process for preparing the same
    2.
    发明授权
    Recombinant anti-HIV antibody and process for preparing the same 失效
    重组抗HIV抗体及其制备方法

    公开(公告)号：US5773247A

    公开(公告)日：1998-06-30

    申请号：US491845

    申请日：1995-07-14

    申请人： Hiroaki Maeda ,  Kazuhiko Kimachi ,  Yasuyuki Eda ,  Kouichi Shiosaki ,  Kiyoshi Osatomi ,  Sachio Tokiyoshi

    发明人： Hiroaki Maeda ,  Kazuhiko Kimachi ,  Yasuyuki Eda ,  Kouichi Shiosaki ,  Kiyoshi Osatomi ,  Sachio Tokiyoshi

    IPC分类号： A61K38/00 ,  A61K39/395 ,  C07K16/10 ,  C12N15/13 ,  C12P21/08 ,  C12P21/06 ,  C07H21/04 ,  C12N5/00

    CPC分类号： C07K16/1063 ,  A61K38/00 ,  C07K2319/00

    摘要： The present invention relates to a gene fragment coding for a variable region of an antibody having a neutralizing activity against human immunodeficiency virus (HIV) and a process for preparing the same. A mouse-human chimeric antibody and a mouse-human reshaped antibody having a neutralizing activity against HIV can be prepared by obtaining a specific nucleotide sequence of a gene fragment coding for a variable region of H chain and L chain of an antibody having a neutralizing activity against HIV, and then artificially fusing DNAs synthesized based on these nucleotide sequences with a gene coding for a human immunoglobulin. The novel recombinant anti-HIV antibody of the present invention is useful for treatment and prevention of AIDS.

    摘要翻译： PCT No.PCT / JP93 / 00039 Sec。 371 1995年7月14日第 102（e）日期1995年7月14日PCT提交1993年1月14日PCT公布。 第WO94 / 15969号公报 日期：1994年7月12日本发明涉及编码对人免疫缺陷病毒（HIV）具有中和活性的抗体的可变区的基因片段及其制备方法。 可以通过获得编码具有中和活性的抗体的H链和L链的可变区的基因片段的特异性碱基序列来制备具有针对HIV的中和活性的小鼠 - 人嵌合抗体和小鼠 - 人重整抗体 然后人造地将基于这些核苷酸序列合成的DNA与编码人免疫球蛋白的基因融合。 本发明的新型重组抗HIV抗体可用于治疗和预防艾滋病。

    Cat-mouse heterohybridoma and gene fragment coding for constant region of feline immunoglobulin
    4.
    发明授权
    Cat-mouse heterohybridoma and gene fragment coding for constant region of feline immunoglobulin 失效
    猫鼠异种杂交瘤和编码猫免疫球蛋白恒定区的基因片段

    公开(公告)号：US5880274A

    公开(公告)日：1999-03-09

    申请号：US559947

    申请日：1995-11-17

    申请人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    发明人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    IPC分类号： C07K16/00 ,  C07K16/08 ,  C07K16/46 ,  C12N5/20 ,  C12N5/26 ,  C12N15/13 ,  C12P21/08 ,  C07H21/04 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C12N5/166 ,  C07K16/00 ,  C07K16/081 ,  C07K16/462 ,  C07K2317/24 ,  C07K2319/00

    摘要： A Gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of feline immunoglobulin .lambda. chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of feline immunoglobulin .kappa. chain; a gene fragment which comprises a DNA sequence coding for the constant region of feline immunoglobulin .gamma. chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-cat chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a feline immunoglobulin wherein the latter gene fragment is linked to the 3' site of the former gene fragment; a polypeptide of a mouse-cat chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule coding for an amino acid sequence of the mouse-cat chimeric antibody is incorporated; a cat-mouse heterohybridoma which produces feline immunoglobulin; and a process for preparing a feline immunoglobulin gene.

    摘要翻译： 一种基因片段，其包含编码猫免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 一种基因片段，其包含编码猫免疫球蛋白κ链的恒定区的氨基酸序列的DNA序列; 包含编码猫免疫球蛋白γ链恒定区的DNA序列的基因片段; 编码小鼠-cat嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段 的猫免疫球蛋白，其中后一个基因片段连接到前一个基因片段的3'位点; 将从编码小鼠-cat嵌合抗体的氨基酸序列的所述重组DNA分子并入的细胞的表达载体转化的细胞中表达的小鼠-cat嵌合抗体的多肽; 产生猫免疫球蛋白的猫 - 鼠异种杂交瘤; 以及制备猫免疫球蛋白基因的方法。

    Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins
    5.
    发明授权
    Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins 失效
    犬小鼠异源杂交瘤和编码犬免疫球蛋白恒定区的基因片段

    公开(公告)号：US5852183A

    公开(公告)日：1998-12-22

    申请号：US646981

    申请日：1996-05-08

    申请人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    发明人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    IPC分类号： A61K39/00 ,  C07K16/46 ,  C12N5/26 ,  C12N15/13 ,  C07H2/04 ,  C07H21/02

    CPC分类号： C07K16/462 ,  A61K39/00 ,  C07K2317/24

    摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin wherein the latter gene fragment; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.

    摘要翻译： 一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段 的犬免疫球蛋白，其中后者基因片段; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

    Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins
    7.
    发明授权
    Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins 失效
    犬小鼠异源杂交瘤和编码犬免疫球蛋白恒定区的基因片段

    公开(公告)号：US5593861A

    公开(公告)日：1997-01-14

    申请号：US306520

    申请日：1994-09-15

    申请人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    发明人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

    IPC分类号： A61K39/00 ,  C07K16/46 ,  C12N5/26 ,  C12N15/13 ,  C12N15/00 ,  C07H21/04 ,  C12P21/04 ,  C12P21/06

    CPC分类号： C07K16/462 ,  A61K39/00 ,  C07K2317/24

    摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.

    摘要翻译： 一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段 的犬免疫球蛋白; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

    Anti-feline calicivirus recombinant antibody and gene fragment encoding the same
    8.
    发明授权
    Anti-feline calicivirus recombinant antibody and gene fragment encoding the same 失效
    抗猫杯状病毒重组抗体和编码相同的基因片段

    公开(公告)号：US5785968A

    公开(公告)日：1998-07-28

    申请号：US24253

    申请日：1993-03-01

    申请人： Kazuhiko Kimachi ,  Hiroaki Maeda ,  Kiyoto Nishiyama ,  Sachio Tokiyoshi ,  Yukinobu Tohya ,  Takeshi Mikami

    发明人： Kazuhiko Kimachi ,  Hiroaki Maeda ,  Kiyoto Nishiyama ,  Sachio Tokiyoshi ,  Yukinobu Tohya ,  Takeshi Mikami

    IPC分类号： A61K38/00 ,  C07K16/10 ,  C12N15/13 ,  C12P21/08 ,  C07H21/04 ,  A61K39/395 ,  C07H16/00

    CPC分类号： C07K16/1045 ,  A61K38/00

    摘要： Anti-FCV (feline calicivirus) feline-type recombinant antibody effective for treatment, prevention and diagnosis of FCV infection and a gene fragment useful for preparation of said antibody are provided. Cell line 1D7 capable of producing a mouse monoclonal antibody having an excellent FCV-neutralizing activity was constructed and a gene fragment coding for the V region in charge of the FCV-specific binding of said antibody was obtained. This gene fragment and the gene coding for the constant region of the feline antibody are used to give a chimeric anti-FCV recombinant antibody. The obtained recombinant antibody is a novel antibody and is useful for the diagnosis, treatment and prevention of feline virus infections, particularly feline calicivirus infection, with high safety in administration into cats.

    摘要翻译： 提供了有效治疗，预防和诊断FCV感染的抗FCV（猫杯状病毒）猫科动物重组抗体和用于制备所述抗体的基因片段。 构建能够产生具有优异的FCV中和活性的小鼠单克隆抗体的细胞系1D7，得到编码负责所述抗体的FCV特异性结合的V区的基因片段。 使用该基因片段和编码猫抗体恒定区的基因得到嵌合抗FCV重组抗体。 获得的重组抗体是一种新的抗体，可用于诊断，治疗和预防猫病毒感染，特别是猫杯状病毒感染，对猫的施用具有高度的安全性。

    Method for producing exogenous protein
    10.
    发明授权
    Method for producing exogenous protein 失效
    生产外源蛋白的方法

    公开(公告)号：US5683891A

    公开(公告)日：1997-11-04

    申请号：US436462

    申请日：1995-05-26

    申请人： Kiyoto Nishiyama ,  Yuji Ishikawa ,  Kazuhiko Kimachi ,  Hiroaki Maeda ,  Sachio Tokiyoshi

    发明人： Kiyoto Nishiyama ,  Yuji Ishikawa ,  Kazuhiko Kimachi ,  Hiroaki Maeda ,  Sachio Tokiyoshi

    IPC分类号： C07K16/00 ,  C12N15/85 ,  C12P21/08 ,  C07K16/44 ,  C12N15/06 ,  C12N15/13

    CPC分类号： C12N15/85 ,  C07K16/00 ,  C12N2840/20 ,  C12N2840/44

    摘要： A novel method for production of an exogenous protein is provided, the method being suitable for expression of an exogenous protein, especially a recombinant antibody etc., in an eucaryotic cell by utilizing a genetic recombination technique. That is, a novel method for production of an exogenous protein is provided which allows for culture of a cell in which an exogenous gene is introduced in a serum-free medium and an efficient production of an exogenous protein, by using, as a host cell for expression, a fused cell which is prepared by fusing a mouse myeloma and a lymphatic cell and which can be cultured in a serum-free medium.

    摘要翻译： PCT No.PCT / JP95 / 01723 Sec。 371日期：1995年5月26日 102（e）日期1995年5月26日PCT提交1993年11月25日PCT公布。 公开号WO94 / 12658 日期1994年6月9日提供了一种生产外源蛋白质的新方法，该方法适用于通过利用遗传重组技术在真核细胞中表达外源蛋白，特别是重组抗体等。 也就是说，提供了一种用于生产外源蛋白质的新方法，其允许通过使用作为宿主细胞来培养外源基因导入无血清培养基和有效产生外源蛋白质的细胞 用于表达融合细胞，其通过融合小鼠骨髓瘤和淋巴细胞并可在无血清培养基中培养而制备。