公开(公告)号：US07615362B2

公开(公告)日：2009-11-10

申请号：US11851522

申请日：2007-09-07

申请人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

发明人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/26 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2482 ,  C12Y302/01008

摘要： A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有广泛pH范围的反应的变异的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第41位氨基酸或第21位氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第二十一个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US07749743B2

公开(公告)日：2010-07-06

申请号：US11851593

申请日：2007-09-07

申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

IPC分类号： C12N9/24

CPC分类号： C12N9/2485 ,  C12Y302/01032

摘要： An isolated xylanase gene with mutations includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the isolated xylanase gene. A site-specific mutagenesis method includes: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the isolated xylanase gene.

摘要翻译： 具有突变的分离的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成分离的木聚糖酶基因。 位点特异性诱变方法包括：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成分离的木聚糖酶基因。

公开(公告)号：US20080254526A1

公开(公告)日：2008-10-16

申请号：US11851522

申请日：2007-09-07

申请人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

发明人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

IPC分类号： C12N9/26

CPC分类号： C12N9/2482 ,  C12Y302/01008

摘要： A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有广泛pH范围的反应的变异的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第41位氨基酸或第21位氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第二十一个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US20080254539A1

公开(公告)日：2008-10-16

申请号：US11851593

申请日：2007-09-07

申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

IPC分类号： C12N15/01 ,  C07H21/00

CPC分类号： C12N9/2485 ,  C12Y302/01032

摘要： A mutated xylanase gene with high reaction activity includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有高反应活性的突变的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US07635471B2

公开(公告)日：2009-12-22

申请号：US11506891

申请日：2006-08-21

申请人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

发明人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

IPC分类号： A61K38/47 ,  A61K38/54 ,  C12N9/42 ,  C12N9/28 ,  C12N9/36 ,  C07H21/04

CPC分类号： C12N9/2434 ,  A61K38/47

摘要： A xylanase gene, denoted xynR8, encoding a xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermostable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

摘要翻译： 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是热稳定的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。

公开(公告)号：US20080118491A1

公开(公告)日：2008-05-22

申请号：US11506891

申请日：2006-08-21

申请人： Kou-Joan Cheng ,  Yo-Chia Chen

发明人： Kou-Joan Cheng ,  Yo-Chia Chen

IPC分类号： A61K38/47 ,  C07H21/04 ,  C12N1/10 ,  C12N1/14 ,  C12N5/04 ,  C12N5/06 ,  C12N7/01 ,  C12N9/28 ,  C12N9/36 ,  C12Q1/68

CPC分类号： C12N9/2434 ,  A61K38/47

摘要： A xylanase gene, denoted xynR8, encoding a novel xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermo-tolerable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

摘要翻译： 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的新型木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是耐热的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。

公开(公告)号：US08518687B2

公开(公告)日：2013-08-27

申请号：US13424501

申请日：2012-03-20

申请人： Yo-Chia Chen ,  Jeng-Chen Liu ,  Hau-Yu Wang ,  Teng-Chieh Hsu ,  Gia-Luen Guo ,  Wen-Song Hwang ,  Jia-Baau Wang

发明人： Yo-Chia Chen ,  Jeng-Chen Liu ,  Hau-Yu Wang ,  Teng-Chieh Hsu ,  Gia-Luen Guo ,  Wen-Song Hwang ,  Jia-Baau Wang

IPC分类号： C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12Y302/01091 ,  C12N9/2437

摘要： A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

摘要翻译： 通过筛选用Orp​​inomyces sp。构建的cDNA基因组文库获得纤维素水解酶及其基因。 Y102。 该基因长1071个碱基对并且包括用于通过翻译产生包含357个氨基酸的纤维素水解酶的开放阅读框（ORF）。 引入转化细胞和携带该基因的载体。 通过转化将基因转移到大肠杆菌中，使得大肠杆菌可以获得分解CMC，β-葡聚糖和木聚糖的活性。 纤维素水解酶是多功能的，并且能够分解纤维素和将纤维直接分解成葡萄糖。

公开(公告)号：US20130102051A1

公开(公告)日：2013-04-25

申请号：US13424501

申请日：2012-03-20

申请人： Yo-Chia Chen ,  Jeng-Chen Liu ,  Hau-Yu Wang ,  Teng-Chieh Hsu ,  Gia-Luen Guo ,  Wen-Song Hwang ,  Jia-Baau Wang

发明人： Yo-Chia Chen ,  Jeng-Chen Liu ,  Hau-Yu Wang ,  Teng-Chieh Hsu ,  Gia-Luen Guo ,  Wen-Song Hwang ,  Jia-Baau Wang

IPC分类号： C12N9/42 ,  C12N1/21 ,  C12N15/56

CPC分类号： C12Y302/01091 ,  C12N9/2437

摘要： A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

摘要翻译： 通过筛选用Orp​​inomyces sp。构建的cDNA基因组文库获得纤维素水解酶及其基因。 Y102。 该基因长1071个碱基对并且包括用于通过翻译产生包含357个氨基酸的纤维素水解酶的开放阅读框（ORF）。 引入转化细胞和携带该基因的载体。 通过转化将基因转移到大肠杆菌中，使得大肠杆菌可以获得分解CMC，β-葡聚糖和木聚糖的活性。 纤维素水解酶是多功能的，并且能够分解纤维素和将纤维直接分解成葡萄糖。