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    Chemical reaction device, chemical reaction system and chemical reaction method
    1.
    发明申请
    Chemical reaction device, chemical reaction system and chemical reaction method 失效
    化学反应装置，化学反应体系和化学反应方法

    公开(公告)号：US20050014246A1

    公开(公告)日：2005-01-20

    申请号：US10854018

    申请日：2004-05-26

    申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  G01N33/558

    CPC分类号： G01N33/54366

    摘要： A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.

    摘要翻译： 提供化学反应装置用于固定在固相上的分子和溶液中的分子之间的化学反应，并且还提供化学分析装置，以通过化学反应和随后的固定在固相上的分子捕获溶液中的分子 捕获的分子的测量。 从而提高了反应效率以及样品通量。 化学反应装置和化学分析装置使用用于反应容器的微流体装置的通道，并且至少一个特定的分子固定在内表面上，并且固定结构或非固定的阻碍流动的障碍物 渠道。 在具有固定在毛细管的内表面上的酶的典型的反应容器和作为填充在毛细管的通道中的流动的障碍物的玻璃珠上，反应溶液可以在一个方向或两个方向上来回移动 从而与固定在内表面上的酶进行反应。 反应溶液的流动不是层流，使得固定的特定分子与反应溶液之间的反应以高效率进行。

    Chemical reaction device, chemical reaction system, and chemical reaction method
    2.
    发明申请
    Chemical reaction device, chemical reaction system, and chemical reaction method 失效
    化学反应装置，化学反应体系和化学反应方法

    公开(公告)号：US20100184237A1

    公开(公告)日：2010-07-22

    申请号：US12654717

    申请日：2009-12-30

    申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    IPC分类号： G01N33/543 ,  F28D21/00 ,  G01N33/00

    CPC分类号： G01N33/54366

    摘要： A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.

    摘要翻译： 提供化学反应装置用于固定在固相上的分子和溶液中的分子之间的化学反应，并且还提供化学分析装置，以通过化学反应和随后的固定在固相上的分子捕获溶液中的分子 捕获的分子的测量。 从而提高了反应效率以及样品通量。 化学反应装置和化学分析装置使用用于反应容器的微流体装置的通道，并且至少一个特定的分子固定在内表面上，并且固定结构或非固定的阻碍流动的障碍物 渠道。 在具有固定在毛细管的内表面上的酶的典型的反应容器和作为填充在毛细管的通道中的流动的障碍物的玻璃珠上，反应溶液可以在一个方向或两个方向上来回移动 从而与固定在内表面上的酶进行反应。 反应溶液的流动不是层流，使得固定的特定分子与反应溶液之间的反应以高效率进行。

    Chemical reaction device, chemical reaction system, and chemical reaction method
    4.
    发明授权
    Chemical reaction device, chemical reaction system, and chemical reaction method 失效
    化学反应装置，化学反应体系和化学反应方法

    公开(公告)号：US07964388B2

    公开(公告)日：2011-06-21

    申请号：US12654717

    申请日：2009-12-30

    申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    IPC分类号： C12M1/34

    CPC分类号： G01N33/54366

    摘要： A device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution. A chemical analysis device is also provided to capture molecules in the solution and subsequently measure the captured molecules. Reaction efficiency and sample throughput are thereby improved. The chemical reaction and chemical analysis devices use a microfluidic device channel as a reaction vessel. The channel is provided with a particular molecule immobilized on an interior surface of the channel with an obstacle positioned against the flow. In a typical reaction vessel having an enzyme immobilized in the capillary's interior surface and glass beads as obstacles, a reaction solution can move either in one direction or back and forth to react with the immobilized enzyme. The flow of the reaction solution is not laminar such that a reaction between the particular molecule and the reaction solution proceeds at high efficiency.

    摘要翻译： 提供了用于固定在固相上的分子与溶液中的分子之间的化学反应的装置。 还提供化学分析装置以捕获溶液中的分子并随后测量捕获的分子。 从而提高反应效率和样品通过量。 化学反应和化学分析装置使用微流体装置通道作为反应容器。 该通道设置有固定在通道的内表面上的特定分子，并具有抵抗流动的位置。 在具有固定在毛细管内表面中的酶和玻璃珠作为障碍物的典型反应容器中，反应溶液可以在一个方向或来回移动以与固定化酶反应。 反应溶液的流动不是层流，使得特定分子和反应溶液之间的反应以高效率进行。

    Chemical reaction device, chemical reaction system and chemical reaction method
    5.
    发明授权
    Chemical reaction device, chemical reaction system and chemical reaction method 失效
    化学反应装置，化学反应体系和化学反应方法

    公开(公告)号：US07666662B2

    公开(公告)日：2010-02-23

    申请号：US10854018

    申请日：2004-05-26

    申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    IPC分类号： C12M1/34

    CPC分类号： G01N33/54366

    摘要： A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.

    摘要翻译： 提供化学反应装置用于固定在固相上的分子和溶液中的分子之间的化学反应，并且还提供化学分析装置，以通过化学反应和随后的固定在固相上的分子捕获溶液中的分子 捕获的分子的测量。 从而提高了反应效率以及样品通量。 化学反应装置和化学分析装置使用用于反应容器的微流体装置的通道，并且至少一个特定的分子固定在内表面上，并且固定结构或非固定的阻碍流动的障碍物 渠道。 在具有固定在毛细管的内表面上的酶的典型的反应容器和作为填充在毛细管的通道中的流动的障碍物的玻璃珠上，反应溶液可以在一个方向或两个方向上来回移动 从而与固定在内表面上的酶进行反应。 反应溶液的流动不是层流，使得固定的特定分子与反应溶液之间的反应以高效率进行。

    On-line chemical reaction system
    6.
    发明申请
    On-line chemical reaction system 审中-公开
    在线化学反应体系

    公开(公告)号：US20050250145A1

    公开(公告)日：2005-11-10

    申请号：US11122045

    申请日：2005-05-05

    申请人： Atsumu Hirabayashi ,  Yoshinobu Kohara ,  Kazunori Okano

    发明人： Atsumu Hirabayashi ,  Yoshinobu Kohara ,  Kazunori Okano

    IPC分类号： G01N27/62 ,  B01J19/00 ,  C12M1/40 ,  C12Q1/68 ,  G01N1/34 ,  G01N1/40 ,  G01N30/06 ,  G01N30/72 ,  G01N35/10

    CPC分类号： G01N35/1097 ,  G01N1/40 ,  G01N1/405

    摘要： In enzymatic reaction carried out batch-wise, loss of the sample cannot be ignored, and according to the conventional technologies aiming at diminishment of the loss of the sample, a long time is required for reactions. In the present invention, the reaction part in which a chemical substance is immobilized is filled with a sample solution, and the sample solution is held between air at both ends for inhibition of mixing with a buffer solution. The sample solution is provided utilizing a sample introduction part, etc.

    摘要翻译： 在分批进行的酶反应中，不能忽视样品的损失，并且根据旨在减少样品损失的常规技术，反应需要很长时间。 在本发明中，固定有化学物质的反应部分填充有样品溶液，并将样品溶液保持在两端的空气中，以抑制与缓冲溶液的混合。 使用样品引入部件等提供样品溶液

    Method of genetic testing
    7.
    发明申请
    Method of genetic testing 审中-公开
    基因检测方法

    公开(公告)号：US20050287549A1

    公开(公告)日：2005-12-29

    申请号：US11041456

    申请日：2005-01-25

    申请人： Keiichi Nagai ,  Kazunori Okano ,  Hideyuki Noda ,  Hiroko Matsunaga ,  Kiyomi Taniguchi ,  Yoshiaki Yazawa ,  Tomoharu Kajiyama

    发明人： Keiichi Nagai ,  Kazunori Okano ,  Hideyuki Noda ,  Hiroko Matsunaga ,  Kiyomi Taniguchi ,  Yoshiaki Yazawa ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/537 ,  C12Q2565/301 ,  C12Q2525/155

    摘要： This invention provides a method of genetic testing that enables testing of a plurality of variation sites (SNPs) in a cost-effective and simple manner, allowing realization of genetic diagnosis in clinical settings. The SNP type of the nucleic acid sample is evaluated by: allowing a nucleic acid sample having an anchor sequence at its 5′ end to hybridize to a support having, immobilized on its surface, a probe containing a sequence that is complementary to the target sequence (the SNP region); extending a complementary strand from the probe utilizing the nucleic acid sample as a template; dissociating and removing the nucleic acid sample from the extended probe; extending a complementary strand using the extended probe as a template and a primer having a sequence identical to the anchor sequence; and detecting pyrophosphoric acid generated via the primer extension, based on bioluminescence.

    摘要翻译： 本发明提供了一种能够以成本有效和简单的方式测试多个变异位点（SNP）的遗传测试方法，从而允许在临床环境中实现遗传诊断。 核酸样品的SNP类型通过以下方式评估：使具有其5'端的锚定序列的核酸样品与固定在其表面上的载体杂交，所述载体固定有包含与靶序列互补的序列的探针 （SNP地区）; 使用核酸样品作为模板从探针延伸互补链; 从扩展的探针中分离并除去核酸样品; 使用扩展探针作为模板和具有与锚定序列相同的序列的引物延伸互补链; 并基于生物发光检测通过引物延伸产生的焦磷酸。

    Cell measuring and sorting chip
    8.
    发明授权
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US08361412B2

    公开(公告)日：2013-01-29

    申请号：US11317004

    申请日：2005-12-27

    申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC分类号： B01L3/00

    CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

    摘要翻译： 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。