Modification of peptide and protein
    1.
    发明授权
    Modification of peptide and protein 失效
    肽和蛋白质的修饰

    公开(公告)号:US6010871A

    公开(公告)日:2000-01-04

    申请号:US809593

    申请日:1997-09-08

    摘要: A process for modifying a physiologically active peptide or a physiologically active protein which comprises reacting a physiologically active peptide or a physiologically active protein having at least a glutamine residue with a substance having an amino donor in the presence of a transglutaminase originating in a microorganism to thereby form an acid amide bond at the .gamma.-position acid amide group of the glutamine residue with the amino group of the amino donor; and the product of modification obtained thereby.

    摘要翻译: PCT No.PCT / JP95 / 01994 Sec。 371日期:1997年9月8日 102(e)1997年9月8日PCT PCT 1995年9月29日PCT公布。 WO96 / 10089 PCT出版物 日期1996年4月4日一种改变生理活性肽或生理活性蛋白质的方法,其包括使生理活性肽或具有至少谷氨酰胺残基的生理活性蛋白质与具有氨基供体的物质在转谷氨酰胺酶的存在下反应 从而在氨基供体的氨基的谷氨酰胺残基的γ位酸酰胺基上形成酰胺键; 和由此获得的改性产物。

    Transglutaminase originating from Crassostrea gigas
    3.
    发明授权
    Transglutaminase originating from Crassostrea gigas 失效
    源自Crassostrea gigas的转谷氨酰胺酶

    公开(公告)号:US5736356A

    公开(公告)日:1998-04-07

    申请号:US525654

    申请日:1995-09-28

    CPC分类号: C12N9/1044

    摘要: The present invention relates to a transglutaminase originating from Crassostrea gigas, a gene coding for the transglutaminase, a plasmid carrying the gene, a microorganism transformed with the plasmid, a method for producing an intended transglutaminase by cultivating the microorganism and a method for gelating a protein using the transglutaminase originating from Crassostrea gigas. When comparing with other transglutaminases, the transglutaminase originating from Crassostrea gigas has novel characteristic properties such that it can be activated by the action of calcium ions and that it is further activated by the addition of sodium chloride and/or potassium chloride and it is of utility value, in particular, as a gelling agent for foods.

    摘要翻译: PCT No.PCT / JP95 / 00117 Sec。 371 1995年9月28日第 102(e)1995年9月28日PCT PCT 1995年1月30日PCT公布。 公开号WO95 / 20662 日期1995年8月3日本发明涉及来自Crassostrea gigas的转谷氨酰胺酶,编码转谷氨酰胺酶的基因,携带该基因的质粒,用质粒转化的微生物,通过培养微生物生产预期的转谷氨酰胺酶的方法和 使用源自Crassostrea gigas的转谷氨酰胺凝胶化蛋白质的方法。 当与其他转谷氨酰胺酶相比时,源自Crassostrea gigas的转谷氨酰胺酶具有新颖的特征,使其可以通过钙离子的作用而被活化,并且通过加入氯化钠和/或氯化钾进一步活化,并且其具有效用 价值,特别是作为食品的胶凝剂。

    Gene encoding transglutaminase derived from fish
    4.
    发明授权
    Gene encoding transglutaminase derived from fish 失效
    从鱼衍生的基因编码转谷氨酰胺酶

    公开(公告)号:US5607849A

    公开(公告)日:1997-03-04

    申请号:US583799

    申请日:1996-01-05

    CPC分类号: C12N9/1044

    摘要: The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.

    摘要翻译: 本发明涉及具有编码具有转谷氨酰胺酶活性的多肽的来源于鱼的基因的DNA片段,包含编码转谷氨酰胺酶的来源于鱼的DNA片段的重组质粒,转化体, 导入编码转谷氨酰胺酶的衍生DNA片段,以及生产转谷氨酰胺酶的方法,包括培养含有编码转谷氨酰胺酶的鱼衍生DNA片段的转化体。

    Gene encoding transglutaminase derived from fish
    5.
    发明授权
    Gene encoding transglutaminase derived from fish 失效
    从鱼衍生的基因编码转谷氨酰胺酶

    公开(公告)号:US5514573A

    公开(公告)日:1996-05-07

    申请号:US164839

    申请日:1993-12-09

    CPC分类号: C12N9/1044

    摘要: The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.

    摘要翻译: 本发明涉及具有编码具有转谷氨酰胺酶活性的多肽的来源于鱼的基因的DNA片段,包含编码转谷氨酰胺酶的来源于鱼的DNA片段的重组质粒,转化体, 导入编码转谷氨酰胺酶的衍生DNA片段,以及生产转谷氨酰胺酶的方法,包括培养含有编码转谷氨酰胺酶的鱼衍生DNA片段的转化体。