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    DEVICE AND METHOD FOR DIFFERENTIATING TARGET CELL
    1.
    发明申请
    DEVICE AND METHOD FOR DIFFERENTIATING TARGET CELL 审中-公开
    用于鉴别目标细胞的装置和方法

    公开(公告)号：US20130055473A1

    公开(公告)日：2013-02-28

    申请号：US13591571

    申请日：2012-08-22

    申请人： Young-nam KWON ,  Kee-won KIM ,  Min-seok KIM ,  Hyouk-soo HAN ,  Jeong-gun LEE ,  Hee-goo KIM

    发明人： Young-nam KWON ,  Kee-won KIM ,  Min-seok KIM ,  Hyouk-soo HAN ,  Jeong-gun LEE ,  Hee-goo KIM

    IPC分类号： G01Q20/00

    CPC分类号： G01Q30/04 ,  G01Q60/42

    摘要： A device for differentiating a target cell includes a cantilever including a fixed end and a free end, where the cantilever is elastically deformable, a tip disposed on the free end of the cantilever, where the tip contacts a surface of a cell, a measurement unit connected to the fixed end of the cantilever, where the measurement unit measures a degree of a repulsive force based on an elastic deformation of the cantilever, and a conversion unit which converts the repulsive force measured by the measurement unit into a modulus of elasticity derived from the surface of the cell.

    摘要翻译： 用于区分目标单元的装置包括悬臂，其包括固定端和自由端，其中悬臂可弹性变形，设置在悬臂的自由端上的尖端，其中尖端接触单元表面，测量单元 连接到悬臂的固定端，其中测量单元基于悬臂的弹性变形来测量排斥力的程度;以及转换单元，其将由测量单元测量的排斥力转换为由 电池的表面。

    APPARATUS AND METHOD FOR PURIFICATION OF NUCLEIC ACIDS BY PHASE SEPARATION USING LASER AND BEADS
    3.
    发明申请
    APPARATUS AND METHOD FOR PURIFICATION OF NUCLEIC ACIDS BY PHASE SEPARATION USING LASER AND BEADS 审中-公开
    通过激光和珠光相分离纯化核酸的装置和方法

    公开(公告)号：US20080318279A1

    公开(公告)日：2008-12-25

    申请号：US12180842

    申请日：2008-07-28

    申请人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM

    发明人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： B01L3/502761 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0633 ,  G01N35/0098 ,  Y10T436/143333

    摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

    摘要翻译： 提供了用于纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混入毛细管中的振动器; 激光发生器附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。 根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。 使用电磁铁确保去除PCR抑制剂。 此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER
    4.
    发明申请
    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER 审中-公开
    使用微珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US20080199930A1

    公开(公告)日：2008-08-21

    申请号：US12031947

    申请日：2008-02-15

    申请人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    发明人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    IPC分类号： C12N13/00 ,  C12M1/00

    CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    摘要： A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    摘要翻译： 提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。 根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行 或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。

    MICROFLUIDIC VALVE, METHOD OF MANUFACTURING THE SAME, AND MICROFLUIDIC DEVICE COMPRISING THE MICROFLUIDIC VALVE
    8.
    发明申请
    MICROFLUIDIC VALVE, METHOD OF MANUFACTURING THE SAME, AND MICROFLUIDIC DEVICE COMPRISING THE MICROFLUIDIC VALVE 审中-公开
    微流控阀，其制造方法和包含微流控阀的微流体装置

    公开(公告)号：US20110232832A1

    公开(公告)日：2011-09-29

    申请号：US13157535

    申请日：2011-06-10

    申请人： Jong-myeon PARK ,  Yoon-kyoung CHO ,  Beom-seok LEE ,  Jeong-gun LEE

    发明人： Jong-myeon PARK ,  Yoon-kyoung CHO ,  Beom-seok LEE ,  Jeong-gun LEE

    IPC分类号： B21K1/20 ,  H05B6/10 ,  B32B37/00

    CPC分类号： F16K99/0001 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T29/49412 ,  Y10T137/2191 ,  Y10T137/2196

    摘要： Provided is a microfluidic valve, a method of manufacturing the microfluidic valve, and a microfluidic device that employs the microfluidic valve. The microfluidic valve includes a platform that includes two substrates combined facing each other; a channel having a first depth allowing a fluid to flow between the two substrates; a valve gap that is disposed on at least a region of the channel and has a second depth which is smaller than the first depth; and a valve plug that is disposed to fill the valve gap and is formed of a valve material made by mixing a phase change material, which is solid at room temperature, with a plurality of exothermic particles that emit an amount of heat sufficient to melt the phase change material by absorbing electromagnetic waves.

    摘要翻译： 本发明提供一种微流体阀，微流体阀的制造方法和采用该微流体阀的微流体装置。 该微流体阀包括一个平台，该平台包括彼此相结合的两个基板; 具有允许流体在两个基板之间流动的第一深度的通道; 阀间隙，其设置在所述通道的至少一个区域上，并且具有小于所述第一深度的第二深度; 以及阀塞，其设置成填充阀间隙，并且由阀室材料形成，所述阀门材料是通过将在室温下为固体的相变材料与多个放出足以熔化的 相变材料通过吸收电磁波。

    METHOD AND APPARATUS FOR DISRUPTING CELLS AND AMPLIFYING NUCLEIC ACIDS USING GOLD NANORODS
    9.
    发明申请
    METHOD AND APPARATUS FOR DISRUPTING CELLS AND AMPLIFYING NUCLEIC ACIDS USING GOLD NANORODS 有权
    破坏细胞的方法和装置，使用金纳多糖放大核酸

    公开(公告)号：US20110212514A1

    公开(公告)日：2011-09-01

    申请号：US13107677

    申请日：2011-05-13

    申请人： Kwang Ho CHEONG ,  Dong-kee YI ,  Jeong-gun LEE ,  Jong-myeon PARK

    发明人： Kwang Ho CHEONG ,  Dong-kee YI ,  Jeong-gun LEE ,  Jong-myeon PARK

    IPC分类号： C12M1/00 ,  C12M1/02 ,  C12M1/42

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12N13/00 ,  C12P19/34

    摘要： Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber.

    摘要翻译： 提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及 在微室中从破碎的细胞中扩增核酸。