摘要:
As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要:
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要:
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要:
As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要:
The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要:
The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要:
The invention is a crystal oscillator having a vibrating plate comprising a vibrating part surrounded by a rim part spaced from the vibrating part except for a narrow connecting part that connects the vibrating part to the rim part in a cantilever fashion. The thickness of the vibrating part and the connecting part are less than the thickness of the rim part. The vibrating part is sandwiched between a pair of casings. Each of the casings has a recess facing the vibrating part, which prevent the vibrating part from contacting the casings. Each surface facing a recess has an electrode positioned thereon and an electrode lead extending from the surface electrode to the connecting part. Each casing has a through-hole positioned to face an electrical lead. A cup-shaped metal lead is placed in each through-hole with the bottom of the cup contacting the electrical lead facing the through-hole. Current to the surface electrodes is supplied through the metal leads.
摘要:
A process for recovering valuables from a waste etching solution containing a fluorine component and ammonia component is provided comprising recovering respectively a fluorine component and ammonia component in the waste solution and recovering a potassium and/or sodium component used for processing the waste solution, and utilizing respectively the recovered components for preparation of an etching solution or reusing or recycling for processing the waste solution. That is, this process comprises adding at least one of potassium hydroxide and sodium hydroxide to the waste etching solution containing a fluorine component and an ammonia component to form hydroxides of metal ions dissolved in the waste solution, filtering the neutralized solution for separation of the metal hydroxides, heating the filtrate by steam injection to recover the generated ammonia as ammonia gas, aqueous ammonia or liquid ammonia, then subjecting the aqueous solution (KF and/or NaF solution free from ammonia components) after recovering the ammonia component to processing using a monopolar or bipolar membrane to recover the fluorine component as an aqueous hydrofluoric acid solution, further recovering at least one of the potassium component and sodium component as at least one of potassium hydroxide and sodium hydroxide and recycling and reusing the recovered ammonia and recovered aqueous hydrofluoric acid solution for the preparation of a new etching solution and at least one of the recovered potassium hydroxide and sodium hydroxide for processing the waste etching solution as a neutralizing agent.
摘要:
A resistor sheet input tablet comprising two rectangular resistor sheets each provided at two opposite edges thereof with electrodes, the rectangular resistor sheets being superposed such that the electrodes on one of the resistor sheets lie perpendicularly to those on the other resistor sheets, wherein the resistor sheets have a two resistor layer construction comprising (a) a main resistor layer consisting of a thin metal film deposited thereon which in turn is bonded to (b) an electrically insulating layer and (c) a protective resistor layer formed on the surface of the main resistor layer.
摘要:
A printed circuit board having juxtaposed thereon in parallel plural conductive paths, at least a part of which has a different length, extending from one end side to the other end side, wherein the electric resistances of the conductive paths are made substantially same by changing the width of at least a part of the conductive paths.