-
1.发明授权Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof 有权用于使用动物细胞大量生产来自外来基因的蛋白质的表达载体及其用途公开(公告)号：US09096878B2
公开(公告)日：2015-08-04
申请号：US13140905
申请日：2009-12-22
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少 常用的哺乳动物密码子 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
-
2.发明申请EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF 有权用动物细胞生产大量外源基因衍生蛋白的表达载体及其用途公开(公告)号：US20120122083A1
公开(公告)日：2012-05-17
申请号：US13140905
申请日：2009-12-22
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少 常用的哺乳动物密码子 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
-
3.发明申请PROTEIN SUBSTANCE HAVING TRIPLE HELIX STRUCTURE AND MANUFACTURING METHOD THEREFOR 有权具有三重螺旋结构的蛋白质物质及其制造方法公开(公告)号：US20120059152A1
公开(公告)日：2012-03-08
申请号：US13141138
申请日：2009-12-22
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译： 作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。 具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。 通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原， 大批量。
-
4.发明授权Protein substance having triple helix structure and manufacturing method therefor 有权具有三螺旋结构的蛋白质物质及其制造方法公开(公告)号：US08470555B2
公开(公告)日：2013-06-25
申请号：US13141138
申请日：2009-12-22
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译： 作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。 具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。 通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原， 大批量。
-
5.发明申请EXPRESSION VECTOR FOR MASS PRODUCTION OF FOREIGN GENE-DERIVED PROTEIN USING ANIMAL CELL AND USE THEREOF 有权使用动物细胞大量生产外源基因衍生蛋白的表达载体及其用途公开(公告)号：US20110123993A1
公开(公告)日：2011-05-26
申请号：US12918776
申请日：2009-02-27
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱 哺乳动物 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
-
6.发明授权Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof 有权用于使用动物细胞大规模生产外源基因衍生蛋白质的表达载体及其用途公开(公告)号：US08653249B2
公开(公告)日：2014-02-18
申请号：US12918776
申请日：2009-02-27
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱 哺乳动物 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
-
7.发明授权公开(公告)号：US08950027B2
公开(公告)日：2015-02-10
申请号：US13518140
申请日：2010-12-21
IPC分类号： A61F5/56
摘要： A respiratory tract widening tool capable of reliably managing a respiratory tract in a supine posture and freely changing the posture, and a respiratory tract widening unit provided therewith are provided.
摘要翻译： 一种呼吸道加宽工具，其能够以仰卧姿态可靠地管理呼吸道并且自由地改变姿势，并且提供了一种呼吸道加宽单元。
-
8.发明申请公开(公告)号：US20130025058A1
公开(公告)日：2013-01-31
申请号：US13518140
申请日：2010-12-21
摘要： A respiratory tract widening tool capable of reliably managing a respiratory tract in a supine posture and freely changing the posture, and a respiratory tract widening unit provided therewith are provided.
摘要翻译： 一种呼吸道加宽工具，其能够以仰卧姿态可靠地管理呼吸道并且自由地改变姿势，并且提供具有呼吸道加宽单元。
-
公开(公告)号：US20130155030A1
公开(公告)日：2013-06-20
申请号：US13820584
申请日：2011-07-13
申请人： Michihiro Kawai , Keiichi Yamamoto
发明人： Michihiro Kawai , Keiichi Yamamoto
IPC分类号： G06F3/042
CPC分类号： G06F3/042 , G06F3/0428
摘要： A display system displays objects in a display region in a manner arranged in a predetermined orientation. One light-emitting element of a sensor array emits light to the objects in the predetermined orientation. The display system prestores data which associates numerical value ranges different from each other with the objects, respectively. When light reflected by a finger is received by light-receiving elements of the sensor array, the display system calculates the number of the light-receiving elements that have received the reflected light, identifies one object associated with the numerical value range including the calculated number of the light-receiving elements, from among the objects, based on the calculated number of the light-receiving elements and the stored data, and performs processing corresponding to the identified object.
摘要翻译： 显示系统以预定方向布置的方式在显示区域中显示对象。 传感器阵列的一个发光元件以预定取向向对象发光。 显示系统预先分别将数据值彼此不同的数据与对象相关联。 当由手指反射的光被传感器阵列的光接收元件接收时，显示系统计算已经接收到反射光的光接收元件的数量，识别与包括计算出的数字的数值范围相关联的一个对象 的光接收元件，基于计算出的光接收元件数量和存储的数据，并且执行与所识别的对象相对应的处理。
-
公开(公告)号：US08456854B2
公开(公告)日：2013-06-04
申请号：US12285632
申请日：2008-10-09
申请人： Shigeo Iriguchi , Kiyoyuki Hatanaka , Satoshi Watanabe , Nobuo Taketomi , Keiichi Yamamoto , Masaru Sugie
发明人： Shigeo Iriguchi , Kiyoyuki Hatanaka , Satoshi Watanabe , Nobuo Taketomi , Keiichi Yamamoto , Masaru Sugie
CPC分类号： H05K3/3494 , H05K1/0212 , H05K3/225 , H05K2203/101 , H05K2203/176 , Y10T29/49137
摘要： A repair system which prevents heating of weakly heat resistant devices together and causing deterioration of the quality when preheating a first surface of the circuit board, wherein an electromagnetic induction material is buried in advance inside the circuit board near a specific electronic device envisioned as needed repair when becoming a defective electronic device in a production process and an electromagnetic coil emitting electromagnetic waves to an electromagnetic induction member in the vicinity of the repair device is provided and the heat generated by the electromagnetic induction member due to the electromagnetic waves enables the repair device to be heated and detached from the circuit board.
-
-
-
-
-
-
-
-
-
搜索结果
164 条记录 (耗时 0.028 秒)