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    Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
    2.
    发明授权
    Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof 失效
    2S白蛋白基因及其来自葡萄的启动子的核苷酸序列及其用途

    公开(公告)号：US07250296B2

    公开(公告)日：2007-07-31

    申请号：US10382066

    申请日：2003-03-05

    申请人： Zhijian T. Li ,  Dennis J. Gray

    发明人： Zhijian T. Li ,  Dennis J. Gray

    IPC分类号： C12N15/82 ,  C12N15/33 ,  A01H5/00

    CPC分类号： C12N15/8234 ,  C07K14/415

    摘要： The subject invention pertains to the isolation of nucleotide sequences of the 2S albumin gene and its promoter from grape. Promoter sequences of the invention provide for seed-specific transcription of operably linked polynucleotide sequences. Seed-specific expression activity of the subject promoter was also characterized by using transient green fluorescent protein (GFP) expression analysis in transformed somatic embryos (SE) of grape (cv. Thompson Seedless). The subject invention also concerns expression constructs comprising a promoter of the present invention. Plants, plant tissues, and plant cells bred to contain or transformed with a polynucleotide of the invention are also contemplated by the present invention.

    摘要翻译： 本发明涉及从葡萄分离2S白蛋白基因及其启动子的核苷酸序列。 本发明的启动子序列提供可操作地连接的多核苷酸序列的种子特异性转录。 通过在葡萄转化的体细胞胚（SE）（cv.Thopson Seedless）中使用瞬时绿色荧光蛋白（GFP）表达分析，也表征了本发明启动子的种子特异性表达活性。 本发明还涉及包含本发明的启动子的表达构建体。 繁殖含有或用本发明的多核苷酸转化的植物，植物组织和植物细胞也被本发明考虑。

    Process for the accelerated production of triploid seeds for seedless watermelon cultivars
    4.
    发明授权
    Process for the accelerated production of triploid seeds for seedless watermelon cultivars 失效
    用于无籽西瓜品种加速生产三倍体种子的方法

    公开(公告)号：US5007198A

    公开(公告)日：1991-04-16

    申请号：US204158

    申请日：1988-06-08

    申请人： Dennis J. Gray ,  Gary W. Elmstrom

    发明人： Dennis J. Gray ,  Gary W. Elmstrom

    IPC分类号： A01H1/02 ,  A01H4/00 ,  A01H5/08

    CPC分类号： A01H4/00 ,  A01H1/02 ,  A01H5/08 ,  Y10S47/01

    摘要： Disclosed here is a novel process which facilitates the rapid and economical production of seedless watermelon seed. The novel process involves cloning desirable tetraploid watermelon parental lines. These parental lines are essential in the production of triploid seed for the seedless watermelon. The subject invention makes possible the use of self-sterile tetraploid parental lines in the production of triploid seed.

    摘要翻译： 这里公开了一种促进无籽西瓜种子快速和经济地生产的新方法。 该新方法涉及克隆所需的四倍体西瓜亲本品系。 这些亲本系对于无籽西瓜的三倍体种子的生产至关重要。 本发明可以在生产三倍体种子中使用自交不育的四倍体亲本品系。

    Selection of fungal resistant grape somatic embryos
    6.
    发明授权
    Selection of fungal resistant grape somatic embryos 失效
    选择真菌抗性葡萄体细胞胚

    公开(公告)号：US07326826B2

    公开(公告)日：2008-02-05

    申请号：US11107959

    申请日：2005-04-15

    申请人： Dennis J. Gray ,  Jayasankar Subramanian ,  Richard E. Litz

    发明人： Dennis J. Gray ,  Jayasankar Subramanian ,  Richard E. Litz

    IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/08 ,  C12N5/04

    CPC分类号： C12N5/04 ,  A01H1/04 ,  A01H4/005 ,  A01H4/008 ,  A01H5/08 ,  A01H6/88

    摘要： The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.

    摘要翻译： 本发明的特征在于使用液体培养基选择对植物病原体具有抗性的葡萄体细胞胚或葡萄植物的方法。 该方法包括以下步骤：（a）在第一液体培养基中培养葡萄胚胎培养物以生长细胞悬浮培养物，第一液体培养基包括植物生长调节剂和来自植物病原体培养物的滤液; （b）从细胞悬浮培养物回收胚胎发生细胞或胚胎发生细胞团; 和（c）在第二液体培养物和第三培养基中培养胚胎发生细胞或胚胎发生细胞团，以选择葡萄体细胞胚或对植物病原体具有抗性的葡萄植物。

    Pathogen-resistant grape plants
    7.
    发明授权
    Pathogen-resistant grape plants 失效
    病原菌抗性葡萄植物

    公开(公告)号：US06995015B1

    公开(公告)日：2006-02-07

    申请号：US09570217

    申请日：2000-05-12

    申请人： Jayasankar Subramanian ,  Zhijian Li ,  Dennis J. Gray

    发明人： Jayasankar Subramanian ,  Zhijian Li ,  Dennis J. Gray

    IPC分类号： A01H1/04

    CPC分类号： C07K14/415 ,  C12N15/8282

    摘要： The invention features a method of producing a grape somatic embryo having resistance to a plant pathogen, the method including the steps of (a) culturing a grape somatic embryo in a first liquid culture medium that includes a plant growth regulator and a phytotoxin from a plant pathogen; (b) exchanging the first liquid culture medium for a second liquid culture medium not including the phytotoxin; (c) recovering a living grape cell or grape cell cluster from the second liquid culture, the living cell or cell cluster being resistant to the pathogen; and (d) culturing the grape cell or grape cell cluster in a third culture medium to produce a grape somatic embryo.

    摘要翻译： 本发明的特征在于一种生产对植物病原体具有抗性的葡萄体细胞胚的方法，该方法包括以下步骤：（a）在包含植物生长调节剂和来自植物的植物毒素的第一液体培养基中培养葡萄体细胞胚 病原; （b）将不含植物毒素的第二液体培养基的第一液体培养基更换; （c）从第二液体培养物中回收活的葡萄细胞或葡萄细胞簇，活细胞或细胞簇对病原体具有抗性; 和（d）在第三培养基中培养葡萄细胞或葡萄细胞簇以产生葡萄体细胞胚。

    Regeneration system for grape and uses thereof
    8.
    发明授权
    Regeneration system for grape and uses thereof 失效
    葡萄再生系统及其用途

    公开(公告)号：US06455312B1

    公开(公告)日：2002-09-24

    申请号：US09311823

    申请日：1999-05-13

    申请人： Dennis J. Gray ,  Jayasankar Subramanian ,  Richard E. Litz

    发明人： Dennis J. Gray ,  Jayasankar Subramanian ,  Richard E. Litz

    IPC分类号： C12N500

    CPC分类号： C12N5/04 ,  A01H1/04 ,  A01H4/005 ,  A01H4/008 ,  A01H5/08 ,  A01H6/88

    摘要： The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.

    摘要翻译： 本发明的特征在于从胚胎发生细胞或胚发生培养物中产生植物或成熟体细胞胚的方法。 该方法包括以下步骤：（a）提供包含胚胎发生细胞的液体培养物; （b）从培养物中回收胚胎发生细胞; （c）将胚胎发生细胞转移到第二培养物; 和（d）从胚胎发生细胞生长成熟的体细胞葡萄胚胎。