公开(公告)号：US20190383794A1

公开(公告)日：2019-12-19

申请号：US16454937

申请日：2019-06-27

Applicant: Creatv MicroTech, Inc.

Inventor： Olga Makarova ,  Cha-Mei Tang ,  Peixuan Zhu ,  Shuhong Li ,  Daniel Adams ,  Platte T. Amstutz

IPC: G01N33/50 ,  G01N1/40 ,  B01L3/00 ,  B01D39/16 ,  B01D29/00 ,  B01L9/00

Abstract: A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells.

公开(公告)号：US10368541B2

公开(公告)日：2019-08-06

申请号：US14776194

申请日：2014-03-13

Applicant: Creatv MicroTech, Inc.

Inventor： Peixuan Zhu ,  Daniel Adams ,  Cha-Mei Tang

IPC: A01N1/02 ,  G01N33/493 ,  G01N33/574

Abstract: A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.

公开(公告)号：US20160178630A1

公开(公告)日：2016-06-23

申请号：US14974889

申请日：2015-12-18

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel L. ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N15/10

CPC classification number: G01N33/57415 ,  G01N15/1456 ,  G01N33/57492 ,  G01N2001/4088 ,  G01N2015/1062 ,  G01N2333/4742 ,  G01N2333/70589

Abstract: Cytokeratin (CK)+/CD45−/DAPI+ cells in the blood of cancer patients have been considered by most as circulating tumor cells (CTCs). Different methods of isolating CTCs results in a wide range of numbers and subgroups of CTCs from same patient. As a result, the clinical significance of the number of CTCs becomes cloudy. Provided herein is methodology to categorize CTCs into morphologically distinct subpopulations, and to use one of the subpopulations in methods for predicting overall survival of a patient having cancer.

Abstract translation: 癌症患者血液中的细胞角蛋白（CK）+ / CD45- / DAPI +细胞被认为是循环肿瘤细胞（CTC）。 分离CTC的不同方法导致来自同一患者的CTC的数量和亚组范围很广。 结果，CTC数量的临床意义变得多云。 本文提供了将CTC分类到形态上不同的亚群的方法，并且在用于预测具有癌症的患者的总体存活的方法中使用一个亚群。

公开(公告)号：US20140315295A1

公开(公告)日：2014-10-23

申请号：US14213183

申请日：2014-03-14

Applicant: Creatv MicroTech, Inc.

Inventor： Olga Makarova ,  Cha-Mei Tang ,  Peixuan Zhu ,  Shuhong Li ,  Daniel Adams ,  Platte T. Amstutz

IPC: G01N1/34 ,  B01D39/16

CPC classification number: G01N1/34 ,  B01D39/1692 ,  B01D2239/0421 ,  G01N33/50

Abstract: A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells.

Abstract translation: 公开了一种具有亲水表面并且适合于基于尺寸的捕获和分析来自全血和其它人体液体的细胞例如循环癌细胞的微过滤器。 过滤材料是可光限定的，允许通过UV光刻形成精密孔。 示例性实施方案提供了一种将微型过滤器与模拟3D体内环境的3D纳米形成相结合的装置，以更好地促进捕获的细胞的生长。

公开(公告)号：US11353459B2

公开(公告)日：2022-06-07

申请号：US16092998

申请日：2017-04-14

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel Adams ,  Cha-Mei Tang

IPC: A61K45/06 ,  A61P35/00 ,  G01N33/574 ,  A61K39/395 ,  C07K16/28

Abstract: Methods of using detection of PD-L1 expression by circulating cancer cells in the screening, monitoring, treatment and diagnosis of cancer in subjects are disclosed. The methods are based on assaying one or more of circulating tumor cells (CTCs), epithelial to mesenchymal transition CTCs (EMTCTCs), cancer associated macrophage-like cells (CAMLs), and cancer associated vascular endothelial cells (CAVEs) isolated from a subject having cancer for PD-L1 expression.

公开(公告)号：US20210132073A1

公开(公告)日：2021-05-06

申请号：US17128807

申请日：2020-12-21

Applicant: Creatv MicroTech, Inc.

Inventor： Cha-Mei TANG ,  Daniel ADAMS

IPC: G01N33/574 ,  C12Q1/6886 ,  C12N5/09 ,  G01N33/569

Abstract: A new sensitive cell biomarker of solid tumors and viral infection is identified in blood. This biomarker can be used to determine presence of carcinomas, sarcomas, and viruses, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US20180238893A1

公开(公告)日：2018-08-23

申请号：US15506439

申请日：2015-08-25

Applicant: Creatv MicroTech, Inc.

Inventor： Cha-Mei TANG ,  Daniel ADAMS

IPC: G01N33/574 ,  C12Q1/6886 ,  G01N33/569

CPC classification number: G01N33/57492 ,  C12N5/0694 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  G01N33/56983 ,  G01N2800/085

Abstract: A new sensitive cell biomarker of solid tumors and viral infection is identified in blood. This biomarker can be used to determine presence of carcinomas, sarcomas, and viruses, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US09658207B2

公开(公告)日：2017-05-23

申请号：US14359467

申请日：2012-11-21

Applicant: Creatv Microtech, Inc.

Inventor： Cha-Mei Tang ,  Yunqi Zhang

IPC: B01D39/16 ,  G01N33/50 ,  B01D29/00 ,  G01N1/40 ,  B01L3/00 ,  B01L9/00

CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

Abstract: A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. Unique filter holder designs and methods appropriate to hold microfilters to collect the rare cells and allow performing assays in the filter holder are provided. The invention also describes the use of the microfilter and filter holder to collect rare cells from body fluids and perform assays. Rare cells collected on the microfilter in accordance with embodiments of the present invention can be used for medical and biological research applications.

公开(公告)号：US20100279322A1

公开(公告)日：2010-11-04

申请号：US12773370

申请日：2010-05-04

Applicant: Cha-Mei Tang ,  Peixuan Zhu ,  Daniel L. Adams

Inventor： Cha-Mei Tang ,  Peixuan Zhu ,  Daniel L. Adams

IPC: C12Q1/06 ,  G01N33/552 ,  G01N33/574 ,  G01N33/53 ,  G01N33/569 ,  G01N33/551

CPC classification number: G01N33/569 ,  C12Q1/04 ,  G01N33/54313 ,  G01N33/574 ,  G01N33/582

Abstract: A method of detecting target cells and pathogens in a test sample concentrates to target cells in solution by filtering or capturing the target cells on a solid support. The target cells are tagged with a fluorescent dye and dispersed in a solution or suspension. The resulting solution or suspension are introduced to a fluorometer to specifically identify and quantitate the target cells. The target cells can be lysed or whole when introduced to the fluorometer.

Abstract translation: 在测试样品中检测靶细胞和病原体的方法通过在固体支持物上过滤或捕获靶细胞而浓缩以靶向溶液中的细胞。 靶细胞用荧光染料标记并分散在溶液或悬浮液中。 将所得溶液或悬浮液引入荧光计以特异性鉴定和定量靶细胞。 当引入荧光计时，靶细胞可以裂解或整合。

公开(公告)号：US06987836B2

公开(公告)日：2006-01-17

申请号：US10060399

申请日：2002-02-01

Applicant: Cha-Mei Tang ,  Olga V. Makarova

Inventor： Cha-Mei Tang ,  Olga V. Makarova

IPC: G21K1/00

CPC classification number: G21K1/025

Abstract: Grids and collimators, for use with electromagnetic energy emitting devices, include at least a metal layer that is formed, for example, by electroplating/electroforming or casting. The metal layer includes top and bottom surfaces, and a plurality of solid integrated walls. Each of the solid integrated walls extends from the top to bottom surface and has a plurality of side surfaces. The side surfaces of the solid integrated walls are arranged to define a plurality of openings extending entirely through the layer. At least some of the walls also can include projections extending into the respective openings formed by the walls. The projections can be of various shapes and sizes, and are arranged so that a total amount of wall material intersected by a line propagating in a direction along an edge of the grid is substantially the same as another total amount of wall material intersected by another line propagating in another direction substantially parallel to the edge of the grid at any distance from the edge. Methods to fabricate these grids using copper, lead, nickel, gold, any other electroplating/electroforming materials or low melting temperature metals are described.