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    DOWN-REGULATING GENE EXPRESSION IN INSECT PESTS
    4.
    发明申请
    DOWN-REGULATING GENE EXPRESSION IN INSECT PESTS 有权
    下调调控基因表达

    公开(公告)号：US20130291188A1

    公开(公告)日：2013-10-31

    申请号：US13881792

    申请日：2011-10-27

    申请人： Thierry Bogaert ,  Romaan Raemaekers ,  Yann Naudet

    发明人： Thierry Bogaert ,  Romaan Raemaekers ,  Yann Naudet

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8286 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/51

    摘要： The present invention relates to the prevention and/or control of infestation by insect pest species. In particular, the invention relates to down-regulation of expression of target genes in insect pests using interfering ribonucleic acid (RNA) molecules. Also described are transgenic plants that (i) express or are capable of expressing interfering RNAs of the invention and (ii) are resistant to infestation by insect pest species. Compositions containing the interfering RNAs of the invention are also provided.

    摘要翻译： 本发明涉及防治和/或防治害虫物种侵染。 特别地，本发明涉及使用干扰核糖核酸（RNA）分子对害虫中靶基因表达的下调。 还描述了（i）表达或能够表达本发明的干扰RNA的转基因植物，和（ii）对昆虫害虫侵染的抗性。 还提供了含有本发明的干扰RNA的组合物。

    Characterisation of gene function using double stranded RNA inhibition
    8.
    发明申请
    Characterisation of gene function using double stranded RNA inhibition 审中-公开

    公开(公告)号：US20040133943A1

    公开(公告)日：2004-07-08

    申请号：US10738886

    申请日：2003-12-17

    申请人： Devgen NV

    发明人： Geert Plaetinck ,  Christ Platteeuw ,  Katharine Mortier ,  Thierry Bogaert

    IPC分类号： A01H001/00 ,  C12N015/82

    CPC分类号： C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.

    CONTROL OF INSECT PESTS USING RNA MOLECULES
    9.
    发明申请
    CONTROL OF INSECT PESTS USING RNA MOLECULES 有权

    公开(公告)号：US20220170018A1

    公开(公告)日：2022-06-02

    申请号：US17593594

    申请日：2021-03-13

    申请人： DEVGEN NV

    发明人： Lien DE SCHRIJVER ,  Kaat CAPPELLE ,  Lies DEGRAVE ,  Myriam BEGHYN ,  Yann NAUDET

    IPC分类号： C12N15/113 ,  A01N63/60 ,  C12N15/82 ,  A01N63/50

    摘要： Disclosed are double stranded RNA molecules that are toxic to flea beetles, particularly the flea beetle species Psylliodes chrysocephala, Phyllotreta nemorum, Phyllotreta striolata, Phyllotreta armoraciae, Phyllotreta atra and Phyllotreta cruciferae. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.