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    Methods for amplifying nucleic acid using tag-mediated displacement
    5.
    发明授权
    Methods for amplifying nucleic acid using tag-mediated displacement 有权

    公开(公告)号：US11840716B2

    公开(公告)日：2023-12-12

    申请号：US17135878

    申请日：2020-12-28

    申请人： GEN-PROBE INCORPORATED

    发明人： Norman C. Nelson ,  Margarita Batranina-Kaminsky

    IPC分类号： C12P19/34 ,  C12Q1/6855 ,  C12Q1/6844

    CPC分类号： C12P19/34 ,  C12Q1/6855 ,  C12Q1/6844 ,  C12Q2537/137

    摘要： Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

    Detecting
    10.
    发明授权
    Detecting 有权

    公开(公告)号：US11667978B2

    公开(公告)日：2023-06-06

    申请号：US16611806

    申请日：2018-06-06

    申请人： Gen-Probe Incorporated

    发明人： Vanessa Bres ,  Deanna Self ,  Jeffrey M. Linnen

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12Q1/6893 ,  C12Q1/6806

    CPC分类号： C12Q1/6893 ,  C12Q1/6806 ,  C12Q2600/16 ,  C12Q2600/166

    摘要： There is described herein a method for specifically detecting Babesia species nucleic acid in a sample, which in one aspect comprises: (1) contacting a sample, said sample suspected of containing Babesia species nucleic acid, with at least two oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) a first amplification oligomer comprising a first target-hybridizing sequence (i) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:66 and comprises SEQ ID NO:56 or 57; or (ii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:96 and comprises SEQ ID NO:101; or (iii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:97 and comprises SEQ ID NO:101; (iv) comprises or consists of SEQ ID NO:8; (v) comprises or consists of SEQ ID NO:83 and (b) a second amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, and (i) is contained in SEQ ID NO:68 and comprises SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, or SEQ ID NO:85; or (ii) is contained in SEQ ID NO:67 and comprises SEQ ID NO:45 or SEQ ID NO:52; or (iii) is contained in SEQ ID NO:70 and comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, or SEQ ID NO:51; (2) performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and (3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.