公开(公告)号：US4910146A

公开(公告)日：1990-03-20

申请号：US220456

申请日：1988-07-18

申请人： Yossef Tur-Kaspa ,  Robert D. Hartman ,  Kenneth J. Hornacek ,  Kevin J. Brendel

发明人： Yossef Tur-Kaspa ,  Robert D. Hartman ,  Kenneth J. Hornacek ,  Kevin J. Brendel

IPC分类号： A01H4/00

CPC分类号： A01H4/003 ,  A01H4/001

摘要： An automated plant tissue proliferation system in which mature plants within a culture vessel are cut into a plurality of plugs using a die type cutter block. The cutter block is moved using a pick and place actuator to a work station where any extraneous nutrient media or plant material adhering to the sides of the cutter are removed using an indexed membrane sheet. A pair of opposed articulated wiping arms cause the membrane sheet to be wrapped around the cutter and pulled down vertically along its sides to remove the excess material. Thereafter, the cutter is indexed to deposit some fraction of the cut cells into a container having culture medium. Fluid pressure is used to eject material from the appropriate cells onto the media material. A number of additional containers are indexed into position with the cutter until all of the plugs of material are removed. the actuator and ejection system are so operated to distribute the plugs of plant material in a preselected array in a number of containers. The system provides rapid plant proliferation minimizing labor requirements and the liklihood of tissue culture contamination.

摘要翻译： 一种自动植物组织增殖系统，其中使用模型切割器块将培养容器内的成熟植物切割成多个插塞。 使用拾取和放置致动器移动切割器块到工作站，其中附着在切割器侧面的任何外来营养介质或植物材料使用分度膜片去除。 一对相对的铰接擦拭臂使得膜片被卷绕在切割器上并沿其侧面垂直拉下以除去多余的材料。 此后，切割器被转位以将一部分切割的细胞沉积到具有培养基的容器中。 流体压力用于将材料从适当的细胞喷射到介质材料上。 许多附加的容器用刀具被转位到位，直到材料的所有塞子被移除。 致动器和排出系统被如此操作以将植物材料的塞子以预选的阵列分布在多个容器中。 该系统提供了快速的植物繁殖，最大限度地减少了劳动力需求和组织培养污染的可能性。