公开(公告)号：US20240352048A1

公开(公告)日：2024-10-24

申请号：US18760347

申请日：2024-07-01

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Michael Callingham ,  Nikolai Nikolaevich Romanov ,  Xiaohai Liu ,  Justyna Piekos

IPC分类号： C07F5/02 ,  C07H23/00

CPC分类号： C07F5/027 ,  C07H23/00

摘要： The present application relates to substituted dyes containing bis-boron fused heterocycles and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

公开(公告)号：US12091713B2

公开(公告)日：2024-09-17

申请号：US17395294

申请日：2021-08-05

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

发明人： Gary Mark Skinner ,  Geraint Wyn Evans ,  Stanley S. Hong

IPC分类号： C12Q1/6869 ,  G01N21/64

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6439

摘要： An imaging system includes: a sample container including a flowcell that includes a plurality of attachment elements at a plurality of sample locations, wherein a single DNA template molecule is attached to each of the attachment elements, and further wherein an average distance between adjacent attachment elements is less than Abbe's limit; and an imager positioned to image photo-switching occurring at the plurality of attachment elements by capturing on and off events in a plurality of color channels at the same time as the on and off events are occurring for the attached molecules when a stochastic photo-switching chemistry is applied to all of the attached molecules at the same time causing the attached molecules to fluoresce in the on and off events in up to four different colors.

公开(公告)号：US12071711B2

公开(公告)日：2024-08-27

申请号：US17470434

申请日：2021-09-09

申请人： Illumina Cambridge Limited

发明人： Niall Anthony Gormley ,  Geoffrey Paul Smith ,  David Bentley ,  Roberto Rigatti ,  Shujun Luo

IPC分类号： C40B50/06 ,  C12N15/10 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B40/06

CPC分类号： C40B50/06 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B40/06 ,  Y10T436/143333 ,  C12Q1/6855 ,  C12Q2565/525 ,  C12Q2525/313 ,  C12N15/1093 ,  C12Q2525/313 ,  C12Q2565/525

摘要： The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.

公开(公告)号：US12071662B2

公开(公告)日：2024-08-27

申请号：US18313227

申请日：2023-05-05

申请人： Illumina Cambridge Limited

发明人： Elena Cressina ,  Antoine Francais ,  Xiaohai Liu

IPC分类号： C12Q1/68 ,  C07H19/06 ,  C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869

CPC分类号： C12Q1/6869 ,  C07H19/06 ,  C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2535/122

摘要： The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.

公开(公告)号：US12053776B2

公开(公告)日：2024-08-06

申请号：US17854741

申请日：2022-06-30

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

发明人： Geraint Evans ,  Stanley S. Hong

IPC分类号： G01N21/05 ,  B01L3/00 ,  C12Q1/6869 ,  G01N21/64 ,  G02B27/42

CPC分类号： B01L3/502715 ,  G01N21/05 ,  G02B27/4227 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0896 ,  C12Q1/6869 ,  G01N2021/6482

摘要： For example, a flowcell includes: a nanowell layer having a first set of nanowells and a second set of nanowells to receive a sample; a first linear waveguide associated with the first set of nanowells, and a second linear waveguide associated with the second set of nanowells; and a first grating for the first linear waveguide, and a second grating for the second linear waveguide, the first and second gratings providing differential coupling of first light and second light.

公开(公告)号：US20240140939A1

公开(公告)日：2024-05-02

申请号：US18476911

申请日：2023-09-28

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

发明人： Xiaolin WU ,  Benedict MACKWORTH ,  Madushani DHARMARWARDANA ,  Patrick MCCAULEY ,  Philip STACKHOUSE ,  Xiaohai LIU ,  Tao YAN ,  Krystle REISS

IPC分类号： C07D413/04 ,  C12Q1/6869

CPC分类号： C07D413/04 ,  C12Q1/6869

摘要： Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.

公开(公告)号：US20240117409A1

公开(公告)日：2024-04-11

申请号：US18492065

申请日：2023-10-23

申请人： Illumina Cambridge Limited

发明人： Niall Anthony Gormley ,  Louise Fraser ,  Paula Kokko-Gonzales

IPC分类号： C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC分类号： C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

摘要： Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.

公开(公告)号：US11884976B2

公开(公告)日：2024-01-30

申请号：US16515790

申请日：2019-07-18

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC分类号： C12Q1/6874 ,  C08G77/442 ,  C09D153/00 ,  C12Q1/6876 ,  C09D163/00 ,  C08L63/00 ,  C08L33/04

CPC分类号： C12Q1/6874 ,  C08G77/442 ,  C08L33/04 ,  C08L63/00 ,  C09D153/00 ,  C09D163/00 ,  C12Q1/6876

摘要： An example of a resin composition includes a free radical curable resin matrix including an acrylate and a siloxane, and a free radical photoinitiator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.

公开(公告)号：US20240002933A1

公开(公告)日：2024-01-04

申请号：US18343696

申请日：2023-06-28

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Amani El Fagui ,  Wayne N. George

IPC分类号： C12Q1/6874 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2200/16

摘要： An example of a sequencing nanoparticle includes a core of a negatively chargeable, hydrophobic polymer. Alternating layers of a positively charged acrylamide hydrogel and the negatively charged polymer are positioned on the core, wherein the positively charged acrylamide hydrogel forms an outer layer of the sequencing nanoparticle. A negatively charged primer set is attached to the outer layer.

公开(公告)号：US20230392142A1

公开(公告)日：2023-12-07

申请号：US18327187

申请日：2023-06-01

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Grace Desantis ,  Stephen M. Gross ,  Jian-Sen Li ,  Natalie Morrell ,  Andrew Slatter ,  Kevin Shen ,  Samantha Snow

IPC分类号： C12N15/10 ,  C12N11/06 ,  C12Q1/6869 ,  C12Q1/6806

CPC分类号： C12N15/1065 ,  C12N11/06 ,  C12Y207/07 ,  C12Q1/6806 ,  C12Q1/6869

摘要： The present disclosure relates to methods, compositions, and kits for treating target nucleic acids, including methods and compositions for fragmenting and tagging nucleic acid (e.g., DNA) using transposome complexes bound to a solid support.