利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

48 条记录 (耗时 0.075 秒)

    Method for the stable expression of nucleic acids in transgenic plants, controlled by a parsley-ubiquitin promoter
    4.
    发明授权
    Method for the stable expression of nucleic acids in transgenic plants, controlled by a parsley-ubiquitin promoter 有权
    用于在转基因植物中稳定表达核酸的方法，由欧芹泛蛋白启动子控制

    公开(公告)号：US08030539B2

    公开(公告)日：2011-10-04

    申请号：US10515020

    申请日：2003-05-30

    申请人： Gunnar Plesch ,  Marcus Ebneth

    发明人： Gunnar Plesch ,  Marcus Ebneth

    IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/00

    CPC分类号： C12N15/8216

    摘要： The invention relates to a method for the stable expression of nucleic acids in transgenic plants, controlled by a parsley-ubiquitin promoter (PCUbi4-2). Said promoter, isolated from parsley (petroselinum crispum) exhibits in almost all transgenic plants an intensely constitutive expression in almost all vegetable tissue, including the seed. It also contains, among others, a potentially heat-shock inducible element (HSE). The invention also relates to nucleic acid constructs, vectors and transgenic plants and to the use of the latter for producing foodstuffs, animal feed, seeds, pharmaceuticals or fine chemicals.

    摘要翻译： 本发明涉及用于通过欧芹 - 泛素启动子（PCUbi4-2）控制的在转基因植物中稳定表达核酸的方法。 从荷兰芹（petroselinum crispum）分离的所述促进剂在几乎所有的转基因植物中都显示出几乎所有植物组织（包括种子）中的强烈组成型表达。 它还包含一种潜在的热休克诱导因子（HSE）。 本发明还涉及核酸构建体，载体和转基因植物以及后者用于生产食品，动物饲料，种子，药物或精细化学品的用途。

    MEANS AND METHOD FOR DIAGNOSING DIABETES
    6.
    发明申请
    MEANS AND METHOD FOR DIAGNOSING DIABETES 失效
    用于诊断糖尿病的手段和方法

    公开(公告)号：US20100163720A1

    公开(公告)日：2010-07-01

    申请号：US12293598

    申请日：2007-03-21

    申请人： Bianca Bethan ,  Kristina Busch ,  Jan Wiemer ,  Martijn Gipmans ,  Edgar Leibold ,  Jochen Spranger ,  Thomas Bobbert ,  Andreas Friedrich Hermann Pfeiffer

    发明人： Bianca Bethan ,  Kristina Busch ,  Jan Wiemer ,  Martijn Gipmans ,  Edgar Leibold ,  Jochen Spranger ,  Thomas Bobbert ,  Andreas Friedrich Hermann Pfeiffer

    IPC分类号： G01N33/48 ,  H01J49/26

    CPC分类号： G01N33/92 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2800/042 ,  G01N2800/52 ,  Y10T436/144444 ,  Y10T436/24

    摘要： The present invention relates to a method for diagnosing diabetes or a predisposition thereof comprising determining at least one metabolite in a test sample of a subject suspected to suffer from diabetes or to have a predisposition therefor and comparing said at least one metabolite to a reference, whereby diabetes or a predisposition therefor is to be diagnosed. Moreover, the present invention encompasses a collection of metabolites, a data collection comprising characteristic values of metabolites and a storage medium comprising said data collection. Furthermore, the present invention also relates to a system comprising means for comparing characteristic values of metabolites of a sample operatively linked to a data storage medium. Further encompassed by the present invention are diagnostic means comprising at least one metabolite and the use of said at least one metabolite for the manufacture of diagnostic means for diagnosing diabetes. Finally, the present invention pertains to a method for identifying diabetes-related metabolites.

    摘要翻译： 本发明涉及一种用于诊断糖尿病或其易感性的方法，包括测定怀疑患有糖尿病的受试者的测试样品中的至少一种代谢物或具有易感性并将所述至少一种代谢物与参考对比，由此 要诊断糖尿病或其倾向。 此外，本发明包括代谢物的集合，包括代谢物的特征值的数据收集和包含所述数据收集的存储介质。 此外，本发明还涉及一种系统，其包括用于比较可操作地连接到数据存储介质的样品的代谢物的特征值的装置。 本发明进一步包括的是包含至少一种代谢物和所述至少一种代谢物用于制造用于诊断糖尿病的诊断装置的诊断方法。 最后，本发明涉及鉴定糖尿病相关代谢物的方法。

    Mass spectrometry method for analyzing mixtures of substances
    9.
    发明授权
    Mass spectrometry method for analyzing mixtures of substances 有权
    用于分析物质混合物的质谱法

    公开(公告)号：US07196323B2

    公开(公告)日：2007-03-27

    申请号：US10505154

    申请日：2003-02-10

    申请人： Tilmann B. Walk ,  Martin Dostler

    发明人： Tilmann B. Walk ,  Martin Dostler

    IPC分类号： H01J49/42

    CPC分类号： H01J49/005 ,  H01J49/421

    摘要： The invention relates to a mass spectrometry method for analysing mixtures of substances using a triple quadrupole mass spectrometer, whereby said mixtures of substances are ionised prior to analysis. The invention is characterised in that the method comprises the following steps: a) selection of a mass/charge quotient (m/z) of an ion created by ionisation in a first analytical quadrupole (I) of the mass spectrometer; b) fragmentation of the ion selected in step (a) by applying an acceleration voltage in an additional subsequent quadrupole (II), which is filled with a collision gas and acts as a collision chamber; c) selection of a mass/charge quotient of an ion created by the fragmentation process in step (b) in an additional subsequent quadrupole (III), whereby steps (a) to (c) of the method are carried out at least once; and d) analysis of the mass/charge quotients of all the ions present in the mixture of substances as a result of the ionisation process, whereby the quadrupole (II) is filled with collision gas, but no acceleration voltage is applied during the analysis. Steps (a) to (c) and step (d) can also be carried out in reverse order.

    摘要翻译： 本发明涉及使用三重四极杆质谱仪分析物质混合物的质谱法，其中所述物质混合物在分析之前被电离。 本发明的特征在于该方法包括以下步骤：a）选择在质谱仪的第一分析四极（I）中由电离产生的离子的质量/电荷商（m / z） b）在步骤（a）中选择的离子的碎裂通过在附加的随后的四极（II）中施加加速电压，其填充有碰撞气体并用作碰撞室; c）在另外的随后的四极（III）中选择由步骤（b）中的碎裂过程产生的离子的质量/电荷商，其中该方法的步骤（a）至（c）至少进行一次; 和d）由于电离过程导致物质混合物中存在的所有离子的质量/电荷分数的分析，由此四极（II）填充有碰撞气体，但在分析期间不施加加速电压。 步骤（a）至（c）和步骤（d）也可以以相反的顺序进行。

    Means and Methods for Determining a Clearance Normalized Amount of a Metabolite Disease Biomarker in a Sample
    10.
    发明申请
    Means and Methods for Determining a Clearance Normalized Amount of a Metabolite Disease Biomarker in a Sample 审中-公开
    用于测定样品中代谢物疾病生物标记物的清除标准化量的方法和方法

    公开(公告)号：US20160047829A1

    公开(公告)日：2016-02-18

    申请号：US14435952

    申请日：2013-10-16

    申请人： METANOMICS GMBH ,  REPRECHT-KARLS-UNIVERSITÄT HEIDELBERG

    发明人： Jens Fuhrmann ,  Jenny Fischer ,  Regina Reszka ,  Hugo A Katus ,  Nobert Frey ,  Johanna Sigl ,  Tanja Weis

    IPC分类号： G01N33/92 ,  G01N33/64 ,  G01N33/50 ,  G01N33/62

    CPC分类号： G01N33/92 ,  G01N33/50 ,  G01N33/62 ,  G01N33/64 ,  G01N33/6896 ,  G01N2405/04 ,  G01N2405/08 ,  G01N2560/00 ,  G01N2800/02 ,  G01N2800/042 ,  G01N2800/2814 ,  G01N2800/32 ,  G01N2800/325 ,  G01N2800/347

    摘要： The present invention relates to a method for determining a clearance normalized amount of a metabolite disease biomarker in a sample including the steps of (a) determining the amount of the disease biomarker in at least a first type of sample of a subject suspected to suffer from the disease, (b) determining the amount of a kidney function biomarker which correlates with the glomerular filtration rate (GFR) in the said at least first type of sample, and (c) determining a clearance normalized amount for the metabolite disease biomarker by normalizing the amount determined for the metabolite disease biomarker in step (a) to the amount of the kidney function biomarker determined in step (b). Moreover, the invention also relates to a method for diagnosing a disease in a subject suspected to suffer therefrom and to a device for determining a clearance normalized amount of a metabolite disease biomarker in a sample.

    摘要翻译： 本发明涉及一种用于确定样品中代谢物疾病生物标志物的清除标准化量的方法，包括以下步骤：（a）确定怀疑患有至少一种受试者的至少第一类样品的疾病生物标志物的量 所述疾病，（b）确定与所述至少第一类型样品中的肾小球滤过率（GFR）相关的肾功能生物标志物的量，和（c）通过归一化来测定代谢物疾病生物标志物的清除标准化量 在步骤（a）中为代谢物疾病生物标志物确定的量与步骤（b）中测定的肾功能生物标志物的量的量。 此外，本发明还涉及用于诊断疑似受试者的疾病的方法以及用于测定样品中代谢物疾病生物标志物的清除标准化量的装置。