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公开(公告)号：US12227737B2
公开(公告)日：2025-02-18
申请号：US18061273
申请日：2022-12-02
Applicant: University Health Network , Sinai Health System
Inventor： Daniel Diniz De Carvalho , Shu Yi Shen , Rajat Singhania , Scott Victor Bratman , Ankur Ravinarayana Chakravarthy
IPC: C12N15/10 , C12Q1/6804 , C12Q1/6806 , C12Q1/6886 , C40B30/04 , G01N33/53
Abstract: There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
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公开(公告)号：US20250032606A1
公开(公告)日：2025-01-30
申请号：US18709405
申请日：2022-11-14
Applicant: David S. CAMPO , Richard URBANOWICZ , Mounir ABOUHAIDAR , UNIVERSITY HEALTH NETWORK , THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO , UNIVERSITY OF LIVERPOOL , CENTERS FOR DISEASE CONTROL AND PREVENTION , THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVIC
Inventor： David S. CAMPO , Richard A. URBANOWICZ , Mounir G. ABOUHAIDAR , Alexander I. MOSA , Jordan J. FELD
IPC: A61K39/29 , A61K39/00 , A61K39/385 , A61P31/14 , C07K14/005 , C12N7/00 , G16B15/30 , G16B40/30
Abstract: There is described herein polyvalent HCV vaccines, preferably comprising SEQ ID Nos. 1-5. There is also described herein methods of designing polyvalent vaccines.
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公开(公告)号：US20250006375A1
公开(公告)日：2025-01-02
申请号：US18820166
申请日：2024-08-29
Applicant: University Health Network
Abstract: This is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
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4.发明申请
公开(公告)号：US20240366090A1
公开(公告)日：2024-11-07
申请号：US18662750
申请日：2024-05-13
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： Ralph S. DaCosta , Brian C. Wilson , Kai Zhang
Abstract: A portable imaging system for visualizing bacteria in a wound in real time includes an excitation light source configured to emit violet and/or blue excitation light to illuminate a wound and cause at least one biomarker associated with the wound to fluoresce and a camera configured to detect fluorescence emitted by the at least one biomarker associated with the wound in response to illumination of the wound with the excitation light. A portable frame is configured to support the camera and the excitation light source for hands-free operation and to position the camera and the excitation light source relative to the wound. The system also includes a power source for the excitation light source.
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公开(公告)号：US20240352535A1
公开(公告)日：2024-10-24
申请号：US18684427
申请日：2022-08-17
Inventor： Paul C. BOUTROS , Michael FRASER
IPC: C12Q1/6886
CPC classification number: C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158
Abstract: Disclosed herein are methods and compositions for treatment, prognosis, and diagnosis of cancer, including prostate cancer. Aspects of the disclosure are directed to methods for a subject having prostate cancer determined to have ZNRF3 genomic loss, reduced ZNRF3 expression, and/or increased ZNRF3 methylation. Also disclosed are methods for analysis of tumor DNA for ZNRF3 copy number status, expression, and/or methylation, as well as compositions and kits useful for such analysis.
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6.发明公开
公开(公告)号：US20240346658A1
公开(公告)日：2024-10-17
申请号：US18629603
申请日：2024-04-08
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： Ralph DACOSTA
CPC classification number: G06T7/0016 , A61B5/0071 , A61B5/0077 , A61B5/445 , G01N21/6486 , G06T2207/10016 , G06T2207/10024 , G06T2207/10048 , G06T2207/10064 , G06T2207/30088 , G06T2207/30096
Abstract: A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.
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公开(公告)号：US20240335478A1
公开(公告)日：2024-10-10
申请号：US18288241
申请日：2022-04-22
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： SHAFIQUE KESHAVJEE , MARCELO CYPEL , MINGYAO LIU , STEPHEN JUVET , KUMI MESAKI
IPC: A61K35/42 , A61K31/52 , A61K31/573 , A61K38/13 , A61P37/06 , C07K14/47 , C07K14/54 , C12N9/22 , C12N15/11 , C12N15/90
CPC classification number: A61K35/42 , A61K31/52 , A61K31/573 , A61K38/13 , A61P37/06 , C07K14/4703 , C07K14/5428 , C12N9/22 , C12N15/11 , C12N15/907 , C12N2310/20
Abstract: The present disclosure relates generally to epigenetically and genetically modified organs and tissues and methods of producing same. In particular, the present disclosure is directed to organs and tissues that have been epigenetically and/or genetically modified at one or multiple loci to control inflammation-regulating or immune-regulating gene expression and thereby improve the condition of the organs and tissues.
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8.发明公开
公开(公告)号：US20240294895A1
公开(公告)日：2024-09-05
申请号：US18441770
申请日：2024-02-14
Inventor： Marcelo CYPEL , Aizhou WANG , Shafique KESHAVJEE , Stephen G. WITHERS , Peter RAHFELD , Jayachandran KIZHAKKEDATHU
CPC classification number: C12N9/80 , A01N1/0226 , C12N9/2402 , C12N11/00 , C12Y302/01049 , C12Y305/01025 , C07K2319/00
Abstract: Provided herein are perfusion fluids for enzymatically cleaving A-antigens from a donor organ, and methods. uses. associated therewith. In particular, the perfusion fluids comprise two enzymes. GalNAcDeacetylase and Galactosaminidase and the fluids may further comprise a buffered extracellular solution and/or a crowing agent. Furthermore. the compositions described herein were found to have activity at temperatures and ph levels suitable for cell viability.
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9.发明公开
公开(公告)号：US20240293029A1
公开(公告)日：2024-09-05
申请号：US18662583
申请日：2024-05-13
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： Ralph S. DaCosta , Brian C. Wilson , Kai Zhang
CPC classification number: A61B5/0071 , A61B5/0059 , A61B5/01 , A61B5/445 , A61B5/72 , A61B5/742 , G01N21/6456 , G01N21/6486 , A61B5/0042 , A61B5/4519 , A61B10/00 , G01N21/6408 , G01N2021/6421 , G01N2021/6471 , G01N2201/0221 , Y02A90/10
Abstract: A method of visualizing bacteria in real time includes positioning a portable frame of a portable imaging system relative to a target, wherein the portable imaging system includes a camera and an excitation light source mounted on the portable frame, operating the portable imaging system in a hands-free manner to illuminate the target with excitation light having at least one wavelength to cause at least one biomarker associated with the target to fluoresce and to detect fluorescence of the at least one biomarker with the camera.
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10.发明公开
公开(公告)号：US20240182966A1
公开(公告)日：2024-06-06
申请号：US18210490
申请日：2023-06-15
Applicant: University Health Network
Inventor： Sowmya Viswanathan , Kevin Robb
IPC: C12Q1/6876 , C12N5/0775
CPC classification number: C12Q1/6876 , C12N5/0663 , C12Q2600/158
Abstract: The present disclosure provides critical quality attributes for assessing immunomodulatory fitness or angiogenic fitness of mesenchymal stromal cells. Also provided is use of these critical quality attributes to determine quantitative values for assessing the fitness of a sample from a particular donor and/or grown using particular critical processing parameters.
