利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.017 秒)

    Nitroreductase enzymatic substrates
    1.
    发明授权
    Nitroreductase enzymatic substrates 有权
    硝基还原酶酶底物

    公开(公告)号：US08883437B2

    公开(公告)日：2014-11-11

    申请号：US13387295

    申请日：2010-07-29

    申请人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    发明人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    IPC分类号： C12Q1/26 ,  C12Q1/04 ,  C07D263/56 ,  C07D209/20 ,  C07D277/62

    CPC分类号： C12Q1/26 ,  C12Q1/04

    摘要： The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; X is NR, CZ5Z6, S or O, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic, Z5 and Z6 being an alkyl; Y is N or N+R, R being alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; Z1, Z2, Z3 and Z4 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.

    摘要翻译： 本发明涉及式（I）化合物作为酶底物用于检测硝基还原酶活性的用途，其中：W1，W2，W3和W4独立地为H，Br，Cl，F，I，烷基，烷氧基 ，硫代甲基，全氟烷基，硝基，氰基，羧基（包括其酯或酰胺）或其任何组合; n = 0,1或2; X是NR，CZ5Z6，S或O，R是H，烷基，芳烷基，芳基，链烷酸或烷基磺酸，Z5和Z6是烷基; Y是N或N + R，R是烷基，芳烷基，芳基，链烷酸或烷基磺酸; Z 1，Z 2，Z 3和Z 4独立地是H，Br，Cl，F，I，烷基，芳基，烷氧基，全氟烷基，硝基，氰基，羧基，磺酰基，包括磺酰基或羧基酰胺或其酯 。

    MICROFLUIDIC PCR DEVICE
    2.
    发明申请
    MICROFLUIDIC PCR DEVICE 有权
    微流感PCR装置

    公开(公告)号：US20140038193A1

    公开(公告)日：2014-02-06

    申请号：US13956677

    申请日：2013-08-01

    申请人： bioMérieux S.A. ,  STMicroelectronics S.r.l.

    发明人： Giuseppe Emanuele SPOTO ,  Luigi Giuseppe OCCHIPINTI ,  Cristian DALL'OGLIO ,  Crocifisso Marco Antonio RENNA ,  Laurent DRAZEK

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0864

    摘要： A microfluidic device (1000-1005), comprising: a semiconductor body (2) having a first side (2a) and a second side (2b) opposite to one another, and housing, at the first side, a plurality of wells (4), having a first depth; an inlet region (30) forming an entrance point for a fluid to be supplied to the wells; a main channel (6a) fluidically connected to the inlet region, and having a second depth; and a plurality of secondary channels (6b) fluidically connecting the main channel to a respective well, and having a third depth. The first depth is higher than the second depth, which in turn is higher than the third depth. According to an aspect, the microfluidic device further comprises a cover layer (8), arranged above the first side of the semiconductor body, configured for sealing the wells and provided with at least a first valve hole (54) which extends through the cover layer and overlaps, at least partially, the secondary channels; and a flexible layer (14), arranged above the cover layer and provided with at least a protrusion (74) extending through the first valve hole towards the semiconductor body and overlapping, at least partially, the secondary channels, the flexible layer being configured such that, when a pressure is applied on it, the protrusion contacts the semiconductor body and enters the secondary channels thus fluidically isolating the wells from one another.

    摘要翻译： 一种微流体装置（1000-1005），包括：具有彼此相对的第一侧（2a）和第二侧（2b）的半导体本体（2），并且在第一侧容纳多个孔（4） ），具有第一深度; 形成用于供应给所述井的流体的入口点的入口区域（30） 与所述入口区流体连接并具有第二深度的主通道（6a） 以及多个次级通道（6b），其将主通道流体连接到相应的井，并且具有第三深度。 第一深度高于第二深度，其又高于第三深度。 根据一个方面，微流体装置还包括覆盖层（8），其布置在半导体主体的第一侧上方，构造成用于密封阱并且设置有至少第一阀孔（54），第一阀孔延伸穿过覆盖层 并且至少部分地与次要信道重叠; 和柔性层（14），其布置在覆盖层上方并且至少设置有延伸穿过第一阀孔朝向半导体主体并且至少部分地与次级沟道重叠的突起（74），柔性层被配置为 当施加压力时，突起与半导体本体接触并进入二次通道，从而将孔彼此流体隔离。

    Method for identifying bacteria from the Bacillus cereus group
    4.
    发明授权
    Method for identifying bacteria from the Bacillus cereus group 有权
    从蜡样芽胞杆菌群鉴定细菌的方法

    公开(公告)号：US08871465B2

    公开(公告)日：2014-10-28

    申请号：US13496422

    申请日：2010-09-16

    申请人： Marie Cellier ,  John Mills ,  David Mosticone ,  Sylvain Orenga ,  Antoine Vimont

    发明人： Marie Cellier ,  John Mills ,  David Mosticone ,  Sylvain Orenga ,  Antoine Vimont

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  C12Q1/44

    摘要： The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.

    摘要翻译： 本发明涉及一种用于鉴定蜡状芽孢杆菌群体细菌的方法，包括以下步骤：（a）提供可含有蜡样芽孢杆菌群细菌的样品，包含至少一种荧光磷脂酰胆碱磷脂酶C（PC -PLC）底物和革兰氏阴性菌的抑制剂; （b）用样品接种反应介质; （c）孵育接种的反应介质; 和（d）通过检测PC-PLC底物水解反应来鉴定蜡状芽孢杆菌群的细菌，其中反应介质的pH和检测PC-PLC底物水解反应所需的时间适应于所述水解反应 在可能存在于样品中的除了属于蜡状芽孢杆菌属的那些之外的任何革兰氏阳性细菌在PC-PLC底物水解之前检测到蜡状芽孢杆菌的细菌。

    Nitroreductase enzymatic substrates
    5.
    发明授权
    Nitroreductase enzymatic substrates 有权
    硝基还原酶酶底物

    公开(公告)号：US08592175B2

    公开(公告)日：2013-11-26

    申请号：US13387280

    申请日：2010-07-29

    申请人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    发明人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    IPC分类号： C12Q1/26 ,  C07K4/00 ,  C07D215/18

    CPC分类号： C12Q1/26 ,  C12Q1/04

    摘要： The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; U is N or N+R and V is CZ6N or N+R or V is N or N+R and U is CZ6, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; and Z1, Z2, Z3, Z4, Z5 and Z6 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.

    摘要翻译： 本发明涉及式（I）化合物作为酶底物用于检测硝基还原酶活性的用途，其中：W1，W2，W3和W4独立地为H，Br，Cl，F，I，烷基，烷氧基 ，硫代甲基，全氟烷基，硝基，氰基，羧基（包括其酯或酰胺）或其任何组合; n = 0,1或2; U是N或N + R，V是CZ6N或N + R或V是N或N + R，U是CZ6，R是H，烷基，芳烷基，芳基，链烷基或烷基磺酸; 并且Z 1，Z 2，Z 3，Z 4，Z 5和Z 6独立地为H，Br，Cl，F，I，烷基，芳基，烷氧基，全氟烷基，硝基，氰基，羧基，磺酰基，包括磺酰基或羧基酰胺或其酯， 和盐的相同。

    Peptidase substrates
    6.
    发明授权
    Peptidase substrates 有权
    肽酶底物

    公开(公告)号：US08592174B2

    公开(公告)日：2013-11-26

    申请号：US13386760

    申请日：2010-07-28

    申请人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    发明人： Olivier Fabrega ,  Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    IPC分类号： C12Q1/37 ,  C07D231/00 ,  A61K38/06

    CPC分类号： G01N33/84 ,  C12Q1/04 ,  C12Q1/37

    摘要： The present invention relates to the use of a compound of the following formula (I), as an enzyme substrate for the detection of a peptidase activity or as a pH indicator: according to which: Y1 is a peptide, H or an alkyl; W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination thereof; n=0, 1 or 2; U is N or N+R and V is CZ6N or N+R or else V is N or N+R and U is CZ6; R is H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z1, Z2, Z3, Z4 and Z5 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof.

    摘要翻译： 本发明涉及下述式（I）化合物作为检测肽酶活性的酶底物或作为pH指示剂的用途：其中：Y1为肽，H或烷基; W1，W2，W3和W4独立地为H，Br，Cl，F，I，烷基，烷氧基，硫代甲基，全氟烷基，硝基，氰基，羧基（包括其酯或酰胺）或其任何组合; n = 0,1或2; U是N或N + R，V是CZ6N或N + R，或者V是N或N + R，U是CZ6; R是H，烷基，芳烷基，芳基，烷酰基或烷基磺酰基; Z 1，Z 2，Z 3，Z 4和Z 5独立地为H，Br，Cl，F，I，烷基，芳基，烷氧基，全氟烷基，硝基，氰基，羧基，磺酰基，包括羧基或磺酰基酯或酰胺及其盐。

    Peptidase substrates
    7.
    发明授权
    Peptidase substrates 有权
    肽酶底物

    公开(公告)号：US08535901B2

    公开(公告)日：2013-09-17

    申请号：US13386578

    申请日：2010-07-28

    申请人： Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    发明人： Arthur James ,  Sylvain Orenga ,  John Perry ,  Vindhya Salwatura ,  Stephen Stanforth

    IPC分类号： C12Q1/37 ,  C07K4/00 ,  C07D213/00

    CPC分类号： C12Q1/37 ,  C12Q1/04 ,  G01N33/84

    摘要： The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: according to which: Y1 is a peptide, H or an alkyl; W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination thereof; n=0, 1 or 2; U and V are N, N+R or CZ4, R being H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z1, Z2, Z3 and Z4 being independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof.

    摘要翻译： 本发明涉及下式（I）的化合物用于检测肽酶活性和/或pH变化的用途：根据该化合物：Y1是肽，H或烷基; W1，W2，W3和W4独立地为H，Br，Cl，F，I，烷基，烷氧基，硫代甲基，全氟烷基，硝基，氰基，羧基（包括其酯或酰胺）或其任何组合; n = 0,1或2; U和V是N，N + R或CZ4，R是H，烷基，芳烷基，芳基，烷酰基或烷基磺酰基; Z 1，Z 2，Z 3和Z 4独立地为H，Br，Cl，F，I，烷基，芳基，烷氧基，全氟烷基，硝基，氰基，羧基，磺酰基，包括羧基或磺酰基酯或酰胺及其盐。

    Protease-stable, cell wall-lysing enzymes
    8.
    发明授权
    Protease-stable, cell wall-lysing enzymes 有权
    蛋白酶稳定，细胞壁裂解酶

    公开(公告)号：US08492519B2

    公开(公告)日：2013-07-23

    申请号：US12674369

    申请日：2008-08-19

    申请人： Holger Grallert ,  Michael Forchheim

    发明人： Holger Grallert ,  Michael Forchheim

    IPC分类号： C07K1/00

    CPC分类号： C12N9/00 ,  A01N63/02 ,  C12N9/503 ,  C12N9/80

    摘要： The present invention relates to a modified polypeptide with a biological activity to lyse cell walls of bacteria, wherein the polypeptide has no caspase, clostripain, enterokinase, factor Xa, granzyme B, staphylococcus peptidase I (V8 Protease), plasmin, streptopain, bacillolysin and/or thrombin cleavage site. The invention further relates to nucleic acids with a sequence encoding a polypeptide according to the present invention.

    摘要翻译： 本发明涉及具有裂解细菌细胞壁的生物学活性的修饰多肽，其中多肽不含半胱天冬酶，clostripain，肠激酶，因子Xa，粒酶B，葡萄球菌肽酶I（V8蛋白酶），纤溶酶，链鸟嘌呤，杆菌溶素和 /或凝血酶切割位点。 本发明还涉及具有编码根据本发明的多肽的序列的核酸。

    Microfluidic PCR device
    9.
    发明授权
    Microfluidic PCR device 有权

    公开(公告)号：US09777317B2

    公开(公告)日：2017-10-03

    申请号：US13956677

    申请日：2013-08-01

    申请人： STMicroelectronics S.r.l. ,  bioMérieux S.A.

    发明人： Giuseppe Emanuele Spoto ,  Luigi Giuseppe Occhipinti ,  Cristian Dall'Oglio ,  Crocifisso Marco Antonio Renna ,  Laurent Drazek

    IPC分类号： C12Q1/68 ,  B01L3/00

    CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0864

    摘要： A microfluidic device (1000-1005), comprising: a semiconductor body (2) having a first side (2a) and a second side (2b) opposite to one another, and housing, at the first side, a plurality of wells (4), having a first depth; an inlet region (30) forming an entrance point for a fluid to be supplied to the wells; a main channel (6a) fluidically connected to the inlet region, and having a second depth; and a plurality of secondary channels (6b) fluidically connecting the main channel to a respective well, and having a third depth. The first depth is higher than the second depth, which in turn is higher than the third depth. According to an aspect, the microfluidic device further comprises a cover layer (8), arranged above the first side of the semiconductor body, configured for sealing the wells and provided with at least a first valve hole (54) which extends through the cover layer and overlaps, at least partially, the secondary channels; and a flexible layer (14), arranged above the cover layer and provided with at least a protrusion (74) extending through the first valve hole towards the semiconductor body and overlapping, at least partially, the secondary channels, the flexible layer being configured such that, when a pressure is applied on it, the protrusion contacts the semiconductor body and enters the secondary channels thus fluidically isolating the wells from one another.