公开(公告)号：US20190204338A1

公开(公告)日：2019-07-04

申请号：US16189848

申请日：2018-11-13

申请人： Phylogica Limited ,  Cambridge Enterprise Limited

发明人： Paul Watt ,  Bryn Hardwick ,  Grahame McKenzie ,  Ashok Venkitaraman

IPC分类号： G01N33/68 ,  G16C20/60 ,  G16B35/00 ,  G16B15/00 ,  C12N15/10 ,  G01N33/50 ,  C07K14/195

CPC分类号： G01N33/6845 ,  C07K14/195 ,  C12N15/1079 ,  G01N33/5041 ,  G01N33/6842 ,  G01N2500/10 ,  G16B15/00 ,  G16B35/00 ,  G16C20/60

摘要： The present invention relates to improved and integrated methods for the characterisation of an interaction site on a target protein that modulates the phenotype of a mammalian cell, such as a phenotype other than death and/or reduced growth. Such methods of the present invention include those to identify a target protein modulates such a phenotype of a mammalian cell, and optionally to characterise an interaction site on said target protein. Such identification and characterisation methods are useful in the development of research tools and/or therapeutics, such protein/peptide or small molecule therapeutics. Accordingly, the present invention also relates to methods of: identification of a ligand, such as a small molecule ligand, that binds to such a target protein; and identification a compound being a candidate modulator of said phenotype of a mammalian cell. The invention further relates to peptides or proteins, or fragments, variants and/or derivatives thereof) comprising certain amino acid sequences, nucleic acids encoding such peptides or proteins and uses of such peptides or proteins or of such nucleic acids.

公开(公告)号：US20180320170A1

公开(公告)日：2018-11-08

申请号：US15909191

申请日：2018-03-01

申请人： enEvolv, Inc.

发明人： Jay H. Konieczka ,  James E. Spoonamore ,  Ilan N. Wapinski ,  Farren J. Isaacs ,  Gregory B. Foley

IPC分类号： C12N15/10 ,  C12N15/81 ,  C12N15/70 ,  C12N15/11 ,  C12N15/85

CPC分类号： C12N15/1082 ,  C12N15/1079 ,  C12N15/111 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2330/31

摘要： The present disclosure provides compositions and methods for genomic engineering.

公开(公告)号：US20180267029A1

公开(公告)日：2018-09-20

申请号：US15938575

申请日：2018-03-28

申请人： Arizona Board of Regents, a body corporate of the State of Arizona, acting for and on behalf of Ariz ,  Engineering Arts LLC

发明人： Peter Wiktor ,  Joshua Labaer ,  Peter Kahn ,  Bharath Takulapalli ,  Ji Qiu ,  Al Brunner ,  Mitch Magee

IPC分类号： G01N33/543 ,  G01N33/68 ,  C12N15/10 ,  C40B60/12 ,  C40B50/14 ,  B01J19/00

CPC分类号： G01N33/543 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12N15/1079 ,  C40B50/14 ,  C40B60/12 ,  G01N33/6845

摘要： Biomolecule arrays on a substrate are described which contain a plurality of biomolecules, such as coding nucleic acids and/or isolated polypeptides, at a plurality of discrete, isolated, locations. The arrays can be used, for example, in high throughput genomics and proteomics for specific uses including, but not limited molecular diagnostics for early detection, diagnosis, treatment, prognosis, monitoring clinical response, and protein crystallography.

公开(公告)号：US20180163197A1

公开(公告)日：2018-06-14

申请号：US15577653

申请日：2016-05-27

申请人： STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS

发明人： Thijn Reinout BRUMMELKAMP ,  Joppe Daniël Maria NIEUWENHUIS ,  Lucas Tilmann JAE ,  Markus BROCKMANN ,  Vincent Arthur BLOMEN ,  Matthijs RAABEN

IPC分类号： C12N15/10 ,  C12N15/01 ,  G01N33/574 ,  C12Q1/6886

CPC分类号： C12N15/1051 ,  C12N15/01 ,  C12N15/1024 ,  C12N15/1079 ,  C12Q1/6886 ,  G01N33/574

摘要： The present invention comprises generally applicable methods for identifying endogenous physiologically relevant genetic elements that affects a intracellular phenotype of interest. In the methods, non-living cells that have been subjected to a mutagenesis treatment are sorted based on phenotype and analyzed to identify the genetic element. By use of these methods, elements previously unknown to be involved in a phenotype can be identified, for example in relationship to health conditions, external stress or drug response, in particular in cancer.

公开(公告)号：US20180119138A1

公开(公告)日：2018-05-03

申请号：US15807007

申请日：2017-11-08

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Daniel E. Bauer ,  Stuart H. Orkin ,  Neville Espi Sanjana ,  Ophir Shalem ,  Jason Wright ,  Feng Zhang

IPC分类号： C12N15/10

CPC分类号： C12N15/1079 ,  C12N15/102 ,  C12N15/1075 ,  C12N15/1082

摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.

公开(公告)号：US09944925B2

公开(公告)日：2018-04-17

申请号：US14909184

申请日：2014-08-04

申请人： enEvolv, Inc.

发明人： Jay H. Konieczka ,  James E. Spoonamore ,  Ilan N. Wapinski ,  Farren J. Isaacs ,  Gregory B. Foley

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/85 ,  C12N15/11 ,  C12N15/70 ,  C12N15/81 ,  C12N15/63

CPC分类号： C12N15/1082 ,  C12N15/1079 ,  C12N15/111 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2330/31

摘要： The present disclosure provides compositions and methods for genomic engineering.

公开(公告)号：US20170369870A1

公开(公告)日：2017-12-28

申请号：US15632222

申请日：2017-06-23

申请人： The Regents of the University of Colorado, a body corporate ,  Muse Biotechnology, Inc.

发明人： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB ,  Marcelo Colika BASSALO ,  Ramsey Ibrahim ZEITOUN

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N2310/20

CPC分类号： C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

摘要： Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US09702012B2

公开(公告)日：2017-07-11

申请号：US12681588

申请日：2008-10-03

申请人： Michael McArthur

发明人： Michael McArthur

IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/689 ,  C12N15/1048 ,  C12N15/1079 ,  C12N15/1086 ,  C12Q1/6811 ,  C12Q1/6897 ,  C12Q2600/136 ,  C12Q2600/158 ,  Y02A50/59 ,  C12Q2522/101 ,  C12Q2525/179 ,  C12Q2537/1373

摘要： Compositions and methods for identifying and using cis-regulatory and decoy sequences.

公开(公告)号：US09683231B2

公开(公告)日：2017-06-20

申请号：US13993930

申请日：2011-12-16

申请人： Hai Jiang ,  Justin Pritchard ,  Douglas A. Lauffenburger ,  Michael Hemann

发明人： Hai Jiang ,  Justin Pritchard ,  Douglas A. Lauffenburger ,  Michael Hemann

IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N33/50

CPC分类号： C12N15/1079 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/178 ,  G01N33/5023

摘要： The invention is directed to a method of characterizing a mechanism of action of an agent (e.g., a chemotherapeutic agent, a genotoxic agent). The method comprises contacting a plurality of populations of cells with an agent to be assessed, wherein each population of cells have one gene of interest targeted by a small hairpin RNA (shRNA) and wherein said gene of interest regulates cell death and a plurality of genes that regulate cell death are targeted in the plurality of populations of cells. A responsiveness of each population of cells to the agent is determined, thereby obtaining an shRNA signature of the agent, so as to identify one or more genes that mediate a response to the agent, thereby characterizing the mechanism of action of the agent. The invention is also directed an article of manufacture for characterizing a mechanism of action of a chemotherapeutic or genotoxic agent.

公开(公告)号：US20160326521A1

公开(公告)日：2016-11-10

申请号：US15110712

申请日：2015-01-09

申请人： Helge ZIELER

发明人： Helge ZIELER

IPC分类号： C12N15/10

CPC分类号： C12N15/1079 ,  C07H21/04 ,  C07K2319/00 ,  C12N15/10 ,  C12N15/74

摘要： The present invention relates to compositions involving randomized in-frame fusion polynucleotides and their introduction into a host organism to identify desirable phenotypic changes. The present invention further relates to methods of generating these randomized in-frame fusion polynucleotides by introducing randomized in-frame fusion polynucleotides into an organism, selecting for organisms with new or altered phenotypes, re-isolating the randomized in-frame fusion polynucleotides from the selected organisms, re-assembling the constituent polynucleotides of the re-isolated randomized in-frame fusion polynucleotides into new collections of randomized in-frame fusion polynucleotides, and repeating the selection for organisms with new or altered phenotypes.

摘要翻译： 本发明涉及涉及随机化的帧内融合多核苷酸并将其引入宿主生物体以鉴定所需表型变化的组合物。 本发明还涉及通过将随机化的帧内融合多核苷酸引入生物体，选择具有新的或改变的表型的生物体来产生这些随机化的框内融合多核苷酸的方法，从所选择的重新分离随机的帧内融合多核苷酸 将重新分离的随机化帧内融合多核苷酸的组成多核苷酸重新组装成随机化的帧内融合多核苷酸的新集合，并重复用新的或改变的表型进行生物体的选择。