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    Corn Seed Comprising Novel Opaque Modifiers and Related Methods
    6.
    发明申请
    Corn Seed Comprising Novel Opaque Modifiers and Related Methods 有权
    包括新型不透明改性剂和相关方法的玉米种子

    公开(公告)号：US20090246350A1

    公开(公告)日：2009-10-01

    申请号：US12413949

    申请日：2009-03-30

    申请人： Thomas Malvar ,  Shihshieh Huang ,  Alessandra Frizzi ,  Mike Kerns

    发明人： Thomas Malvar ,  Shihshieh Huang ,  Alessandra Frizzi ,  Mike Kerns

    IPC分类号： A23L1/36 ,  A01H5/00 ,  A01H1/02 ,  B02C9/00 ,  C07H21/04 ,  A01D45/02

    CPC分类号： C12N15/8254 ,  A01H5/10

    摘要： Novel corn opaque modifier loci, associated molecular markers and methods for obtaining the loci, markers, and resultant seed are provided. Vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also described. Processes for obtaining milled corn seed products from the vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also provided.

    摘要翻译： 提供了新的玉米不透明修饰基因座，相关分子标记和获得基因座，标记物和所得种子的方法。 还描述了含有不透明修饰基因座的具有降低的α-玉米醇溶蛋白储存蛋白质含量的玻璃体种子。 还提供了从含有不透明修饰剂位点的具有降低的α-玉米蛋白储存蛋白质含量的玻璃质种子获得研磨的玉米种子产品的方法。

    Method for altering the nutritional content of plant seed
    7.
    发明授权
    Method for altering the nutritional content of plant seed 失效
    改变植物种子营养成分的方法

    公开(公告)号：US07547820B2

    公开(公告)日：2009-06-16

    申请号：US11264700

    申请日：2005-11-01

    申请人： Julie A. Kirihara ,  Kenneth A. Hibberd ,  Janice Anthony

    发明人： Julie A. Kirihara ,  Kenneth A. Hibberd ,  Janice Anthony

    IPC分类号： C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8251 ,  C07K14/425 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8253 ,  C12N15/8254

    摘要： The invention provides genetically engineered, preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of at least one amino acid essential to the diet of animals, or increasing the starch content, of a plant. One such method involves stably transforming a cell of a plant with an a preselected DNA sequence encoding an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, preferably a seed storage protein which is deficient in at least one amino acid essential to the diet of animals. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide. The transformed cells are used to generate fertile transgenic plants and seeds. Transgenic seeds are characterized by expression of the preselected DNA sequence which results in a substantial inhibition of production of a seed storage protein deficient in at least one amino acid essential to the diet of animals and/or an increase in the weight percent of an amino acid essential to the diet of animals.

    摘要翻译： 本发明提供遗传工程化的预选DNA序列和使用它们改变植物种子营养含量的方法。 本发明的方法涉及增加植物饮食中必需的至少一种氨基酸的重量百分比或增加植物的淀粉含量。 一种这样的方法包括用编码与编码植物种子储存蛋白的信使RNA（mRNA）基本上相同或互补的RNA分子的预选DNA序列稳定转化植物细胞，所述mRNA（mRNA）优选至少缺乏至少 一种对动物饮食必不可少的氨基酸。 一种替代方法使用至少两个预选的DNA序列稳定转化细胞，其中之一编码与编码植物种子储存蛋白的信使RNA（mRNA）基本相同或互补的RNA分子，以及编码预先选择的DNA分子 多肽。 转化细胞用于产生可育转基因植物和种子。 转基因种子的特征在于预选的DNA序列的表达，其导致对动物饮食必需的至少一个氨基酸缺乏的种子储存蛋白的产生的显着抑制和/或氨基酸的重量百分比的增加 对动物的饮食至关重要

    Plant amino acid biosynthetic enzymes
    8.
    发明授权
    Plant amino acid biosynthetic enzymes 有权
    植物氨基酸生物合成酶

    公开(公告)号：US07368633B2

    公开(公告)日：2008-05-06

    申请号：US10917602

    申请日：2004-08-13

    申请人： Saverio Carl Falco ,  Zhan-Bin Liu

    发明人： Saverio Carl Falco ,  Zhan-Bin Liu

    IPC分类号： C12N15/52 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00 ,  A01H1/00

    CPC分类号： C12N9/001 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

    摘要： This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.

    摘要翻译： 本发明涉及编码植物酶的分离的核酸片段，其催化从天冬氨酸生物合成赖氨酸，苏氨酸，甲硫氨酸，半胱氨酸和异亮氨酸的步骤，所述酶选自二氢吡啶二羧酸还原酶，二氨基庚二酸差向异构酶，苏氨酸 合酶，苏氨酸脱氨酶和S-腺苷甲硫氨酸合成酶。 本发明还涉及在正义或反义方向上构建编码全部或部分酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中酶的水平发生变化。

    Enhanced proteins and methods for their use
    9.
    依法登记的发明
    Enhanced proteins and methods for their use 审中-公开
    增强的蛋白质及其使用方法

    公开(公告)号：USH2213H

    公开(公告)日：2008-04-01

    申请号：US40999303

    申请日：2003-04-09

    申请人： MONSANTO TECHNOLOGY LLC

    发明人： OULMASSOV TIM N ,  RAPP WILLIAM D ,  TIAN KAIRONG ,  WICKER JASON A ,  WELSH WILLIAM J ,  WANG XIA ,  DELISLE ROBERT K ,  VENKATESH TYAMAGONDLU V ,  LIANG JIHONG

    IPC分类号： C12P21/06 ,  A01H1/00 ,  C12N5/04 ,  C12N15/82 ,  C12Q1/68

    CPC分类号： C12N15/8254 ,  C12N15/8253

    摘要： The present invention generally relates to the field of plant genetics and protein biochemistry. More specifically, the present invention relates to modified proteins having an increased number of essential amino acids. The present invention provides proteins modified to have an increased number of essential amino acids, nucleic acid sequences encoding the enhanced proteins, and methods of designing, producing, and using the same. The present invention also includes compositions, transformed host cells, transgenic plants, and seeds containing the enhanced proteins, and methods for preparing and using the same.

    摘要翻译： 本发明一般涉及植物遗传学和蛋白质生物化学领域。 更具体地说，本发明涉及具有增加必需氨基酸数量的修饰蛋白质。 本发明提供了修饰为具有增加数量的必需氨基酸的蛋白质，编码增强蛋白质的核酸序列，以及设计，生产和使用该蛋白质的方法。 本发明还包括组合物，转化的宿主细胞，转基因植物和含有增强蛋白质的种子，以及制备和使用该蛋白质的方法。

    Aspartate kinase
    10.
    发明授权
    Aspartate kinase 有权
    天冬氨酸激酶

    公开(公告)号：US07345220B2

    公开(公告)日：2008-03-18

    申请号：US11034569

    申请日：2005-01-13

    申请人： Saverio Carl Falco

    发明人： Saverio Carl Falco

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00

    CPC分类号： C12N9/1217 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

    摘要： This invention relates to an isolated nucleic acid fragment encoding an aspartate kinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aspartate kinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aspartate kinase in a transformed host cell.

    摘要翻译： 本发明涉及编码天冬氨酸激酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分天冬氨酸激酶的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中天冬氨酸激酶水平的改变。