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24 条记录 (耗时 0.016 秒)

    Method for Reducing DNA Impurities in Viral Compositions
    2.
    发明申请
    Method for Reducing DNA Impurities in Viral Compositions 有权
    减少病毒组合物中DNA杂质的方法

    公开(公告)号：US20130203152A1

    公开(公告)日：2013-08-08

    申请号：US13813897

    申请日：2010-08-12

    申请人： Yi Zhang ,  Jinming Dai ,  Yajin Ni

    发明人： Yi Zhang ,  Jinming Dai ,  Yajin Ni

    IPC分类号： C12N7/02 ,  C12N7/06

    CPC分类号： C12N7/02 ,  A61K39/12 ,  A61K2039/525 ,  C12N7/00 ,  C12N7/06 ,  C12N2760/20134 ,  C12N2760/20151

    摘要： A method for purification of viral compositions is provided. In particular, a method for reduction of unwanted residual DNA in a viral composition while retaining the immunogenicity of the virus itself is provided. The resulting immunogenic viral composition is substantially free of residual DNA, and is useful for the manufacture of medical products, such as vaccines designed for human or animal.

    摘要翻译： 提供了病毒组合物的纯化方法。 具体地，提供了在保持病毒本身的免疫原性的同时还原病毒组合物中不需要的残留DNA的方法。 所得的免疫原性病毒组合物基本上不含残留的DNA，并且可用于制造医疗产品，例如为人或动物设计的疫苗。

    Process for the preparation of a rabies vaccine and vaccine obtained by this process
    4.
    发明授权
    Process for the preparation of a rabies vaccine and vaccine obtained by this process 失效
    通过该方法获得的狂犬病疫苗和疫苗的制备方法

    公开(公告)号：US4255520A

    公开(公告)日：1981-03-10

    申请号：US136722

    申请日：1980-04-02

    申请人： Klaus R. Schell

    发明人： Klaus R. Schell

    IPC分类号： A61K39/205

    CPC分类号： A61K39/205 ,  A61K39/12 ,  C12N7/00 ,  A61K2039/5252 ,  C12N2760/20134 ,  C12N2760/20151

    摘要： A method for preparing a rabies vaccine wherein rabies viruses are multiplied in poultry embryos, the heads of the embryos are harvested and the cell extract containing the rabies antigens is separated therefrom for differential centrifugation and density gradient centrifugation, the separated antigen is further processed using conventional methods. This process results in a highly selective concentration of antigens and the production of viruses having a very high activity and freedom from contaminating proteins.

    摘要翻译： 制备狂犬病疫苗的方法，其中狂犬病病毒在家禽胚胎中繁殖，收获胚胎的头部，从其中分离出含有狂犬病抗原的细胞提取物用于差速离心和密度梯度离心，使用常规方法进一步处理分离的抗原 方法。 该方法导致抗原的高选择性浓度和具有非常高的活性并且不受污染蛋白质的病毒的产生。

    Process for Culturing Adherent Cells
    5.
    发明申请
    Process for Culturing Adherent Cells 有权
    培养粘附细胞的方法

    公开(公告)号：US20110151506A1

    公开(公告)日：2011-06-23

    申请号：US12976679

    申请日：2010-12-22

    申请人： Eric Calvosa ,  Nicolas Seve

    发明人： Eric Calvosa ,  Nicolas Seve

    IPC分类号： C12P1/00 ,  C12N5/10

    CPC分类号： C12N5/00 ,  C12N7/00 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2760/20151

    摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

    摘要翻译： 本发明的主题是制备贴壁细胞的方法，包括：a。 将粘附细胞的悬浮液引入培养基中含有微载体的培养容器中; b。 通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞 处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。 收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。 本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。

    Process for culturing adherent cells
    10.
    发明授权
    Process for culturing adherent cells 有权
    培养贴壁细胞的方法

    公开(公告)号：US09145543B2

    公开(公告)日：2015-09-29

    申请号：US12976679

    申请日：2010-12-22

    申请人： Eric Calvosa ,  Nicolas Seve

    发明人： Eric Calvosa ,  Nicolas Seve

    IPC分类号： C12P1/00 ,  C12N1/00 ,  C12N5/00 ,  C12N7/00

    CPC分类号： C12N5/00 ,  C12N7/00 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2760/20151

    摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

    摘要翻译： 本发明的主题是制备贴壁细胞的方法，包括：a。 将粘附细胞的悬浮液引入培养基中含微载体的培养容器中; b。 通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞 处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。 收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。 本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。