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22 条记录 (耗时 0.025 秒)

    PROTEIN
    3.
    发明申请
    PROTEIN 审中-公开
    蛋白

    公开(公告)号：US20160345609A1

    公开(公告)日：2016-12-01

    申请号：US15114914

    申请日：2015-01-30

    申请人： DUPONT NUTRITION BIOSCIENCES APS

    发明人： Rikke Hoeegh Lorentsen ,  Susan Arent Lund ,  Igor Nikolaev ,  Jan Hendrik A. Van Tuijl ,  Bert Koops

    IPC分类号： A23K20/189 ,  C12C11/00 ,  C12P19/02 ,  A23K10/38 ,  C12N9/24 ,  C12P19/14

    CPC分类号： A23K20/189 ,  A23K10/30 ,  A23K10/38 ,  C12C5/004 ,  C12C7/04 ,  C12C11/00 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2485 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01008 ,  Y02E50/17 ,  Y02P60/873

    摘要： The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).

    摘要翻译： 本发明涉及具有木聚糖酶活性的修饰的GH10木聚糖酶或其片段，其中所述修饰的GH10木聚糖酶或其片段与亲本GH10木聚糖酶相比具有增加的热稳定性，母体GH10木聚糖酶在至少两个 以下位置7,33,79,217和298，其中编号基于FveXyn4（SEQ ID No.1）的氨基酸编号。 特别地，根据本发明的改性木聚糖酶具有以下修饰：N7D; T33V; K79Y，V，F，I，L或M（优选K79Y，F或V，更优选Y）; A217Q，E，P，D或M（优选A217Q，E或P，更优选Q）; 和T298Y，F或W（优选Y或F，更优选Y）。

    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3
    4.
    发明申请
    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 有权
    GHG家族可热稳定的β-XYLOSIDASE 3

    公开(公告)号：US20160076017A1

    公开(公告)日：2016-03-17

    申请号：US14854562

    申请日：2015-09-15

    申请人： Honda Motor Co., Ltd.

    发明人： Yasuhiro KONDO ,  Jiro OKUMA ,  Yoshitsugu HIROSE ,  Asuka YAMAGUCHI ,  Migiwa SUDA ,  Tomohiko KATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/24 ,  C12P19/14 ,  C12P19/02 ,  C12N9/42

    CPC分类号： C12N9/2477 ,  C07K14/435 ,  C12N9/2445 ,  C12N9/2485 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01037

    摘要： A thermostable β-xylosidase, having a β-xylosidase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0.

    摘要翻译： 一种具有β-脱氧核苷酸酶催化结构域的热稳定性 - 木糖苷酶，包括：（A）包含SEQ ID NO：1,3或5所示氨基酸序列的多肽，（B）包含氨基酸序列的多肽 其中至少一个氨基酸已被缺失，取代或添加在SEQ ID NO：1,3或5所示的氨基酸序列中，并且具有对对硝基苯基-β-吡喃木糖苷底物的水解活性 至少在80℃和pH4.0的条件下，或（C）包含与SEQ ID NO：1,3或5所示氨基酸序列具有80％或更高的氨基酸序列的氨基酸序列的多肽，并具有水解 至少在80℃和pH4.0的条件下，对对硝基苯基-β-D-吡喃木糖苷的底物的活性。

    BETA-GLUCOSIDASE
    5.
    发明申请
    BETA-GLUCOSIDASE 审中-公开
    β-GLUCOSIDASE

    公开(公告)号：US20150017692A1

    公开(公告)日：2015-01-15

    申请号：US14324340

    申请日：2014-07-07

    申请人： HONDA MOTOR CO., LTD.

    发明人： Maiko TANAKA ,  Shigenobu MITSUZAWA ,  Satoru SHINKAWA ,  Daisuke SHIBATA ,  Takeshi ARA ,  Migiwa TAKEDA

    IPC分类号： C12N9/42 ,  C12P19/02 ,  C12N9/24 ,  C12P19/14

    CPC分类号： C12N9/2445 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2482 ,  C12N9/2485 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  D21C9/005

    摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.

    摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1所示的氨基酸序列具有92％以上序列同一性的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。

    Mutated Xylanase Gene with High Reaction Activity and Site-Specific Mutagenesis Method Thereof
    6.
    发明申请
    Mutated Xylanase Gene with High Reaction Activity and Site-Specific Mutagenesis Method Thereof 有权
    具有高反应活性和位点特异性诱变方法的突变木聚糖酶基因

    公开(公告)号：US20080254539A1

    公开(公告)日：2008-10-16

    申请号：US11851593

    申请日：2007-09-07

    申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

    发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

    IPC分类号： C12N15/01 ,  C07H21/00

    CPC分类号： C12N9/2485 ,  C12Y302/01032

    摘要： A mutated xylanase gene with high reaction activity includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

    摘要翻译： 具有高反应活性的突变的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成突变的木聚糖酶基因。