公开(公告)号：US20060246544A1

公开(公告)日：2006-11-02

申请号：US11396215

申请日：2006-03-30

申请人： Yun Kang ,  Walter Willett ,  Thomas Klimek ,  Basil Campbell ,  Paul Cino ,  Bradley Thomas ,  John Bermel ,  Chun-Chiang Chen

发明人： Yun Kang ,  Walter Willett ,  Thomas Klimek ,  Basil Campbell ,  Paul Cino ,  Bradley Thomas ,  John Bermel ,  Chun-Chiang Chen

IPC分类号： C12P21/06 ,  C12N9/24 ,  C12N5/06 ,  C07K14/53 ,  C07K14/51

CPC分类号： C07K14/535 ,  C07K1/20 ,  C07K14/505 ,  C07K14/51 ,  C07K14/53 ,  C12N9/1081 ,  C12P21/02 ,  C12Y204/99003

摘要： The present invention provides a manufacturing method for the production of peptides that are grown in insect cell lines. The peptides are grown in insect cell cultures that are infected with baculovirus particles in a culture supplemented with a lipid mixture. The peptides are then isolated from the insect cell culture using a method that employs a tangential flow filtration cascade. The isolated peptides are glycopeptides having an insect specific glycosylation pattern. The glycopeptides may then be conjugated to a modifying group via linkage through a glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase.

摘要翻译： 本发明提供了生产在昆虫细胞系中生长的肽的制备方法。 这些肽在补充有脂质混合物的培养物中被杆状病毒颗粒感染的昆虫细胞培养物中生长。 然后使用采用切向流过滤级联的方法从昆虫细胞培养物中分离肽。 分离的肽是具有昆虫特异性糖基化模式的糖肽。 然后可以通过插入在肽和修饰基团之间并且共价连接到肽和修饰基团上的糖基连接基团通过连接将糖肽与修饰基团缀合。 通过糖基转移酶的作用，由糖基化肽形成缀合物。

公开(公告)号：US20190194303A1

公开(公告)日：2019-06-27

申请号：US16150164

申请日：2018-10-02

申请人： Academia Sinica

发明人： Chi-Huey WONG ,  Chung-Yi WU

IPC分类号： C07K16/18 ,  C12P21/00

CPC分类号： C07K16/18 ,  C07K2317/41 ,  C07K2317/50 ,  C12P21/005 ,  C12Y204/01 ,  C12Y204/99003

摘要： Methods for making modified Fc regions of antibodies and antibody fragments, both human and humanized, and having enhanced stability and efficacy, are provided. Antibodies comprising Fc regions with core fucose residues removed, and attached to oligosaccharides comprising terminal sialyl residues, are provided. Antibodies comprising homogeneous glycosylation of Fc regions with specific oligosaccharides are provided. Fc regions conjugated with homogeneous glycoforms of monosaccharides and trisaccharides, are provided. Methods of preparing human antibodies with modified Fc using glycan engineering, are provided.

公开(公告)号：US20180305725A1

公开(公告)日：2018-10-25

申请号：US15954146

申请日：2018-04-16

申请人： Momenta Pharmaceuticals, Inc.

发明人： Naveen Bhatnagar ,  Robin Meccariello ,  Jonathan C. Lansing ,  Daniel Ortiz ,  Hetal Sarvaiya ,  Nathaniel Washburn

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12Q1/48 ,  C07K16/00

CPC分类号： C12P21/005 ,  C07K16/00 ,  C07K2317/41 ,  C07K2317/52 ,  C12N9/1081 ,  C12Q1/48 ,  C12Y204/99001 ,  C12Y204/99003 ,  G01N2333/91091 ,  G01N2440/38

摘要： Glycoproteins having particular sialylation patterns, and methods of making and using such glycoproteins, are described.

公开(公告)号：US20140057316A1

公开(公告)日：2014-02-27

申请号：US13813804

申请日：2011-02-02

申请人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

发明人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

IPC分类号： C12N9/10

CPC分类号： C12N9/1081 ,  C12Y204/99003

摘要： The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-α-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.

摘要翻译： 本发明涉及生产和纯化唾液酸转移酶多肽，特别是N-乙酰半乳糖胺（Gal NAc）-al-2,6-sialyltransferase I（ST6GalNAcI）多肽的方法。 该方法包括在中国仓鼠卵巢（CHO）细胞中产生唾液酸转移酶多肽的步骤，并用色谱步骤的组合纯化多肽。 该方法导致高纯度和活性的唾液酸转移酶多肽的高产量。 获得的唾液酸转移酶，特别是ST6GalNAcI可用于治疗性蛋白质如G-CSF的糖基化。

公开(公告)号：US20160108450A1

公开(公告)日：2016-04-21

申请号：US14787403

申请日：2014-05-01

申请人： MOMENTA PHARMACEUTCALS, INC.

发明人： Naveen BHATNAGAR ,  Robin MECCARIELLO ,  Jonathan C. LANSING ,  Daniel ORTIZ ,  Hetal SARVAIYA ,  Nathaniel WASHBURN

IPC分类号： C12P21/00 ,  C12Q1/48 ,  C07K16/00

CPC分类号： C12P21/005 ,  C07K16/00 ,  C07K2317/41 ,  C07K2317/52 ,  C12N9/1081 ,  C12Q1/48 ,  C12Y204/99001 ,  C12Y204/99003 ,  G01N2333/91091 ,  G01N2440/38

摘要： Glycoproteins having particular sialylation patterns, and methods of making and using such glycoproteins, are described.

摘要翻译： 描述了具有特定唾液酸化模式的糖蛋白，以及制备和使用这种糖蛋白的方法。

公开(公告)号：US09222081B2

公开(公告)日：2015-12-29

申请号：US13813804

申请日：2011-08-02

申请人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

发明人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

IPC分类号： C12P21/06 ,  C12N9/10

CPC分类号： C12N9/1081 ,  C12Y204/99003

摘要： The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-α-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.

摘要翻译： 本发明涉及唾液酸转移酶多肽，特别是N-乙酰半乳糖胺（Gal NAc）-α-2,6-唾液酸转移酶I（ST6GalNAcI）多肽的生产和纯化方法。 该方法包括在中国仓鼠卵巢（CHO）细胞中产生唾液酸转移酶多肽的步骤，并用色谱步骤的组合纯化多肽。 该方法导致高纯度和活性的唾液酸转移酶多肽的高产量。 获得的唾液酸转移酶，特别是ST6GalNAcI可用于治疗性蛋白质如G-CSF的糖基化。