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    Cell for preparing competent cell, method for preparing competent cell and bacterial strain of Escherichia coli
    4.
    发明授权
    Cell for preparing competent cell, method for preparing competent cell and bacterial strain of Escherichia coli 有权
    用于制备感受态细胞的细胞，用于制备感受态细胞和大肠杆菌细菌菌株的方法

    公开(公告)号：US08512996B2

    公开(公告)日：2013-08-20

    申请号：US12979079

    申请日：2010-12-27

    申请人： Jia-Hung Wang ,  Meng-Yin Tsai ,  Ting-Ting Hung ,  Kelly Teng

    发明人： Jia-Hung Wang ,  Meng-Yin Tsai ,  Ting-Ting Hung ,  Kelly Teng

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N15/74 ,  C12N9/00 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Y301/03012 ,  C12N9/16

    摘要： The invention provides a cell for preparing competent cells, wherein the cell is capable of spontaneously accumulating self-producing trehalose therein and the cell is used for the preparation of competent cells. The invention also provides a method for preparing competent cells, including: culturing the cell for preparing the competent cell mentioned previously to obtain a cell suspension; placing the cell suspension into an ice bath; centrifuging the cell suspension to obtain a cell precipitate; mixing a transform reagent with the cell precipitate; and obtaining competent cells suspension.

    摘要翻译： 本发明提供了一种用于制备感受态细胞的细胞，其中细胞能够自发地在其中积累自产海藻糖，并且该细胞用于制备感受态细胞。 本发明还提供一种制备感受态细胞的方法，包括：培养用于制备前述感受态细胞的细胞以获得细胞悬浮液; 将细胞悬浮液置于冰浴中; 离心细胞悬浮液以获得细胞沉淀; 将转化试剂与细胞沉淀物混合; 并获得感受态细胞悬浮液。

    GENE ENCODING TREHALOSE-6-PHOSPHATE PHOSPHATASE AND USE THEREOF
    6.
    发明申请
    GENE ENCODING TREHALOSE-6-PHOSPHATE PHOSPHATASE AND USE THEREOF 审中-公开
    基因编码TREHALOSE-6磷酸酯磷酸酶及其用途

    公开(公告)号：US20090117227A1

    公开(公告)日：2009-05-07

    申请号：US12279376

    申请日：2007-02-01

    申请人： Yoshihiro Nakao ,  Yukiko Kodama ,  Tomoko Shimonaga

    发明人： Yoshihiro Nakao ,  Yukiko Kodama ,  Tomoko Shimonaga

    IPC分类号： C12G1/00 ,  C07H21/04 ,  C07K14/395 ,  A23L1/202 ,  C12Q1/68 ,  C12N15/63 ,  C12N1/19

    CPC分类号： C12Q1/42 ,  C12C12/004 ,  C12C12/006 ,  C12G1/0203 ,  C12N9/16 ,  C12Q1/02 ,  C12R1/85 ,  C12Y301/03012 ,  G01N2333/39

    摘要： The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.

    摘要翻译： 本发明涉及编码海藻糖-6-磷酸磷酸酶的基因及其用途，特别是用于实际使用的具有优异的耐干性和/或低温储存性的酵母，用所述酵母生产的含酒精饮料的方法和方法 用于生产所述饮料。 更具体地说，本发明涉及通过扩增酿酒酵母中编码海藻糖-6-磷酸磷酸酶Tps2p的TPS2基因的表达水平，特别是非抑制性酵母，其酵母对抗低温贮藏的耐干性和/ 特异于啤酒酿造酵母的-ScTPS2基因和用酵母等生产酒精饮料的方法

    Plants having enhanced yield-related traits and a method for making the same
    7.
    发明授权
    Plants having enhanced yield-related traits and a method for making the same 有权
    具有增强的产量相关性状的植物及其制备方法

    公开(公告)号：US09157092B2

    公开(公告)日：2015-10-13

    申请号：US13502643

    申请日：2010-10-14

    申请人： Ana Isabel Sanz Molinero

    发明人： Ana Isabel Sanz Molinero

    IPC分类号： C12N15/82 ,  A01H5/10

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8273 ,  C12Y204/01015 ,  C12Y301/03012 ,  Y02A40/146

    摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a CLC-like (Chloride Channel-like) polypeptide, or an OsBURP-like (BURP-domain containing protein) polypeptide, or an AP2/ERF polypeptide or a protein fusion comprising a TPS (trehalose-6-phosphate synthase) and a TPP (trehalose-6-phosphate phosphatase) polypeptide or modulating one or more nucleic acid encoding a TPS and a TPP enzyme, whether comprised in the same or in separate molecules. The present invention also concerns plants having modulated expression of a nucleic acids and constructs comprising them, useful in performing the methods of the invention.

    摘要翻译： 本发明一般涉及分子生物学领域，涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地，本发明涉及一种通过调节植物中编码CLC样（氯化物通道样）多肽或OsBURP样（含BURP结构域）的核酸的植物中的表达来增强植物中产量相关性状的方法 蛋白质）多肽或包含TPS（海藻糖-6-磷酸合成酶）和TPP（海藻糖-6-磷酸磷酸酶）多肽的AP2 / ERF多肽或蛋白质融合物，或调节一种或多种编码TPS和TPP的核酸 酶，无论是包含在相同还是分开的分子中。 本发明还涉及具有调节核酸表达的植物和包含它们的构建体的植物，其可用于实施本发明的方法。

    Plant genes encoding trehalose metabolism enzymes
    10.
    发明申请
    Plant genes encoding trehalose metabolism enzymes 有权
    植物基因编码海藻糖代谢酶

    公开(公告)号：US20040229364A1

    公开(公告)日：2004-11-18

    申请号：US10365970

    申请日：2003-02-13

    发明人： Perry G. Caimi ,  Saverio Carl Falco ,  Anthony J. Kinney ,  J. Antoni Rafalski ,  Zude Weng

    IPC分类号： C12N015/29 ,  C12P021/00 ,  C12N015/00 ,  C07H021/04 ,  C12N015/82 ,  C12N015/09 ,  C12N015/63 ,  C07K001/00 ,  C07K014/00

    CPC分类号： C12Y301/03012 ,  C07H21/04 ,  C12N9/16 ,  C12N15/8273

    摘要： This invention relates to isolated nucleic acid fragments encoding trehalose metabolism enzymes, more specifically, alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase in a transformed host cell.

    摘要翻译： 本发明涉及编码海藻糖代谢酶，更具体地，α，α-海藻糖酶，α，α-海藻糖 - 磷酸合成酶或海藻糖-6-磷酸磷酸酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码α，α-海藻糖酶，α，α-海藻糖-6-磷酸磷酸酶的全部或部分的重组DNA构建体，其中表达 重组DNA构建体导致转化的宿主细胞中α，α-海藻糖酶，α，α-海藻糖磷酸合酶或海藻糖-6-磷酸磷酸酶水平的变化。