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公开(公告)号：US08916364B2
公开(公告)日：2014-12-23
申请号：US13878979
申请日：2011-10-12
IPC分类号： C07K14/195 , C12N9/78
CPC分类号： C12N9/78 , C07K14/195 , C12P7/40 , C12P7/42 , C12P13/002 , C12Y305/05001
摘要： The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from acidovorax facilis.
摘要翻译： 本发明涉及在腈的反应中具有改善的活性以形成相应的羧酸的腈水解酶，特别是关于2-甲基戊二腈，1-（氰基甲基）环己烷-1-甲腈和苄腈的反应。 根据本发明的腈水解酶与来自酸酵母的腈水解酶有关。
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公开(公告)号：US08614081B2
公开(公告)日：2013-12-24
申请号：US13381155
申请日：2010-07-22
申请人： Anke Krebber , Emily Mundorff , Marissa Mock , Spiros Kambourakis
发明人： Anke Krebber , Emily Mundorff , Marissa Mock , Spiros Kambourakis
CPC分类号： C12N9/78 , C12Y305/05001
摘要： The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
摘要翻译： 本公开涉及具有腈水解酶活性的多肽，编码多肽的多核苷酸以及使用该多肽的方法。
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公开(公告)号：US20120009633A1
公开(公告)日：2012-01-12
申请号：US13238227
申请日：2011-09-21
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US08034595B2
公开(公告)日：2011-10-11
申请号：US11868909
申请日：2007-10-08
申请人： Mark Madden , David Weiner , Jennifer Ann Chaplin
发明人： Mark Madden , David Weiner , Jennifer Ann Chaplin
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US07927846B2
公开(公告)日：2011-04-19
申请号：US12429499
申请日：2009-04-24
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US07875443B2
公开(公告)日：2011-01-25
申请号：US12429478
申请日：2009-04-24
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US20100196976A1
公开(公告)日：2010-08-05
申请号：US12429499
申请日：2009-04-24
IPC分类号： C12P7/42
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US20100009426A1
公开(公告)日：2010-01-14
申请号：US12556528
申请日：2009-09-09
申请人： Grace DeSantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Kelly Chatman , Robert Farwell
发明人： Grace DeSantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Kelly Chatman , Robert Farwell
IPC分类号： C12N9/78
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
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公开(公告)号：US20050272138A1
公开(公告)日：2005-12-08
申请号：US11125592
申请日：2005-05-09
CPC分类号： C12N9/0006 , C12N9/78 , C12N9/96 , C12N11/02 , C12Y305/05001
摘要： The invention relates to a method of preparing cross-linked enzyme particles using a cross-linking agent. According to the invention, the enzyme particles are formed and subsequently cross-linked using a cross-linking agent having at least n reactive groups where N≧3 and a molecular weight of >2,000 Da. The method according to the invention allows for obtaining enzyme particles having a higher enzyme activity than enzyme particles cross-linked according to the state of the art.
摘要翻译： 本发明涉及使用交联剂制备交联酶颗粒的方法。 根据本发明，使用具有至少n个反应性基团的交联剂形成酶颗粒并随后进行交联，其中N> = 3且分子量> 2,000Da。 根据本发明的方法允许获得具有比根据现有技术交联的酶颗粒更高的酶活性的酶颗粒。
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10.发明申请公开(公告)号：US20020012974A1
公开(公告)日：2002-01-31
申请号：US09751299
申请日：2000-12-28
IPC分类号： C12N009/14 , C12P013/04 , C07H021/04
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： In accordance with the present invention there are provided methods for producing enantiomerically pure null-substituted carboxylic acids, such as, for example, null-amino acids and null-hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 根据本发明，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸，所述方法包括将醛或酮与氰化物和氨组合，或 在具有腈水解酶活性的腈水解酶或多肽的存在下，在足以产生羧酸的条件下立即选择性地水解氨基腈或氰醇中间体的铵盐或胺。
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