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公开(公告)号：US20230184787A1
公开(公告)日：2023-06-15
申请号：US17925005
申请日：2021-05-12
Applicant: Nordic Bioscience A/S
Inventor： Joachim Høg Mortensen , Dovile Sinkeviciute , Tina Manon-Jensen , Morten Asser Karsdal , Anne-Christine Bay-Jensen , Signe Holm Nielsen , Annika Hummersgaard Hansen , Jannie Marie Bülow Sand , Diana Julie Leeming , Nicholas Willumsen , Christina Jensen
CPC classification number: G01N33/6893 , C07K16/18 , G01N2333/4727 , G01N2800/56 , G01N2800/7095 , G01N2470/10
Abstract: Disclosed herein are immunoassay methods for detecting and quantifying human neutrophil elastase (HNE) generated fragments of calprotectin in a biofluid sample; and monoclonal antibodies and assay kits for use in such methods. The methods may be used for detecting, monitoring and/or determining the status or severity of a disease characterized by or exhibiting inflammation in a patient.
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公开(公告)号：US20240125784A1
公开(公告)日：2024-04-18
申请号：US18277424
申请日：2022-03-23
Applicant: SEKISUI MEDICAL CO., LTD.
Inventor： Shiomi HORIUCHI MORISHITA , Rikako OGASAWARA , Tomohide ASAI , Osamu MIYAZAKI
IPC: G01N33/574 , G01N33/68
CPC classification number: G01N33/574 , G01N33/6887 , G01N2470/10
Abstract: The problem addressed by the present invention is to provide an immunological assay method using a monoclonal antibody and a kit including the monoclonal antibody, which can be used for assaying ICTP without requiring special facilities. This problem can be solved by a method for immunological assay of type I collagen C-terminal telopeptide in a biological sample, including contacting type I collagen C-terminal telopeptide with a monoclonal antibody that recognizes an amino acid sequence represented by GFDFSFLP (SEQ ID NO: 1) as an epitope.
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3.发明授权
公开(公告)号：US11668711B2
公开(公告)日：2023-06-06
申请号：US16763707
申请日：2018-11-13
Applicant: CORNELL UNIVERSITY
Inventor： Saurabh Mehta , David Erickson , Zhengda Lu
CPC classification number: G01N33/54388 , G01N21/6428 , G01N33/54386 , G01N33/582 , G01N33/6803 , G01N33/82 , G01N33/84 , G01N2021/6441 , G01N2333/4737 , G01N2333/705 , G01N2458/00 , G01N2470/04 , G01N2470/10
Abstract: A diagnostic assay strip includes a first layer that includes an iron mobile labelled specific binding partner that will bind to and iron biomarker from a sample and produce an iron complex and a vitamin A mobile labelled specific binding partner that will bind to a vitamin A biomarker from the sample and produce a vitamin A complex. A second layer includes iron and vitamin A test regions, and a control region. The iron test region has immobilized specific binding partners that will bind to the iron complex. The vitamin A test region has immobilized vitamin A biomarker that will bind to vitamin A mobile labelled specific binding partner, which is not bound to the vitamin A biomarker, passing from the first layer to the second layer. The control region has a moiety which will non-specifically bind to and immobilize the iron and vitamin A labelled specific binding partners. Methods of using the diagnostic assay strip are also discussed.
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公开(公告)号：US20240019423A1
公开(公告)日：2024-01-18
申请号：US17413368
申请日：2019-12-13
Applicant: OSLER DIAGNOSTICS LIMITED
Inventor： Dene Baldwin , Konstantinos Psarros , David William Neil Edington
IPC: G01N33/543
CPC classification number: G01N33/54353 , G01N33/5438 , G01N2470/04 , G01N2470/10
Abstract: A microfluidic device is provided for conducting multiplex immunoassays to detect multiple target analytes in a liquid sample using a set of tags and anti-tags. The device comprises an array of different anti-tags immobilised on a surface of a sensor for measuring a signal. Each anti-tag is arranged to immobilise a tagged antibody or tagged antigen thereby in use separating tagged immune complexes from the liquid phase affecting the signal measured. The device comprises a sensor chamber for tagged immune complexes to form in the liquid phase and one or more detection zones each comprising the array of different anti-tags for immobilising tagged immune complexes. In use, liquid comprising the tagged immune complexes flows or is fluidically driven from the sensor chamber to the detection zone(s).
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公开(公告)号：US20230296621A1
公开(公告)日：2023-09-21
申请号：US17997878
申请日：2021-05-11
Inventor： James A. Wells , Susanna Elledge , Xin Zhou
CPC classification number: G01N33/6854 , C12N9/0069 , C12Y113/12007 , G01N2469/20 , G01N2470/10
Abstract: Protein biosensors and methods of using these sensors to detect anti-SARS-CoV-2 patient antibodies (Abs) in a solution-based, rapid, and quantitative COVID-19 serological assay are provided. In certain aspects, the sensors each comprise a first fusion protein that comprises a first SARS-CoV-2 viral protein and a first peptide fragment of a split reporter protein, and a second fusion protein that comprises a second fusion protein that comprises a second SARS-CoV-2 viral protein domain and a second peptide fragment of the split reporter protein. Only if the test sample comprise SARS-CoV- antibodies, the first and second peptide fragments associate to produce an enzymatically active reporter protein.
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6.发明公开
公开(公告)号：US20230167196A1
公开(公告)日：2023-06-01
申请号：US17779989
申请日：2021-11-09
Applicant: JIANGNAN UNIVERSITY
Inventor： Chuanlai XU , Jingjing YAO , Hua KUANG , Liguang XU , Maozhong SUN , Xiaoling WU , Liqiang LIU , Wei MA , Jianping ZHU , Changlong HAO , Shanshan SONG , Yongming HU , Aihong WU , Lingling GUO , Xinxin XU
CPC classification number: C07K16/44 , G01N33/9486 , C07K2317/92 , G01N2470/10
Abstract: The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.
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公开(公告)号：US20240309015A1
公开(公告)日：2024-09-19
申请号：US18273646
申请日：2022-01-27
Applicant: SHY Therapeutics, LLC
Inventor： Yaron R. Hadari , Michael Schmertzler , Theresa M. Williams , Luca Carta , Rebecca Hutcheson
IPC: C07D495/04 , A61K31/519 , A61K31/5377 , A61K31/541 , A61P21/00 , G01N33/573
CPC classification number: C07D495/04 , A61K31/519 , A61K31/5377 , A61K31/541 , A61P21/00 , G01N33/573 , G01N2333/914 , G01N2470/10 , G01N2500/20
Abstract: Provided herein are methods and corresponding compounds for treating fibrotic disease by administering to a subject a compound of Formula I or pharmaceutically acceptable salt thereof, which inhibits phosphorylation of Smad2/3, according to Phospho-Smad2/3 Inhibition Assay, and is inactive according to MAPK p38 Activation Assay; or a compound of Formula II or III. Also provided are methods and corresponding compounds for treating cancers, inflammatory diseases, rasopathies and autoimmune leukoproliferative disorders.
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公开(公告)号：US20240210405A1
公开(公告)日：2024-06-27
申请号：US17914101
申请日：2021-03-22
Applicant: Nordic Bioscience A/S
Inventor： Nicholas Willumsen , Jeppe Thorlacius-Ussing
IPC: G01N33/574 , C07K16/18 , G01N33/68
CPC classification number: G01N33/57488 , C07K16/18 , G01N33/6887 , C07K2317/34 , G01N2333/78 , G01N2470/10
Abstract: The present invention relates to monoclonal antibodies that target collagen type XIX, and to immunoassays and kits employing the antibodies. The assays of the invention can be used in the diagnosis and monitoring of cancer.
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公开(公告)号：US20240192224A1
公开(公告)日：2024-06-13
申请号：US18553490
申请日：2022-04-01
Applicant: ADITXT, INC.
Inventor： Ge CHEN , Hong LIU , Shahrokh SHABAHANG
IPC: G01N33/68 , G01N33/543 , G01N33/569 , G01N33/58
CPC classification number: G01N33/6854 , G01N33/54313 , G01N33/56983 , G01N33/582 , G01N2469/20 , G01N2470/10
Abstract: The present disclosure relates generally to compositions and methods for assaying antibodies. More specifically, the disclosure relates to assays that detect antibodies that have neutralizing activity against SARS-CoV-2.
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10.发明公开
公开(公告)号：US20230243819A1
公开(公告)日：2023-08-03
申请号：US17790444
申请日：2020-11-04
Applicant: Beijing Strong Biotechnologies, Inc.
Inventor： Jun Gong , Lanping Xiao , Jinxiang Qi , Qifei Zhang , Guili Wang , Xi Liu
IPC: G01N33/543 , C12N9/04 , G01N33/535 , A61K47/64
CPC classification number: G01N33/54386 , C12N9/0006 , C12Y101/01049 , G01N33/535 , A61K47/64 , G01N2470/10
Abstract: Disclosed is a glucose-6-phosphate dehydrogenase mutant and a use thereof in preparing a detection reagent. Compared with a wild-type glucose-6-phosphate dehydrogenase mutant, the glucose-6-phosphate dehydrogenase mutant contains a combination of the following mutations: 56C, 306C, and 454C. A detection kit prepared by using the glucose-6-phosphate dehydrogenase has strong specificity, high sensitivity, convenient operation, a short detection time, accurate quantification, and is suitable for high-throughput detection.
