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    Alpha 4 beta 2 delta gaba-a receptors as a strategy for pms and alcoholism
    3.
    发明申请
    Alpha 4 beta 2 delta gaba-a receptors as a strategy for pms and alcoholism 有权
    Alpha 4 beta 2 delta gaba-a受体作为pms和酒精中毒的策略

    公开(公告)号：US20070081943A1

    公开(公告)日：2007-04-12

    申请号：US10566559

    申请日：2004-07-30

    申请人： Sheryl Smith ,  Keith Williams

    发明人： Sheryl Smith ,  Keith Williams

    IPC分类号： A61K49/00

    CPC分类号： G01N33/9426 ,  A61K49/0004 ,  G01N33/5058 ,  G01N33/948 ,  G01N2800/304 ,  G01N2800/307

    摘要： The present invention is directed to a screening mechanism for identifying members of the general population at increased risk for alcoholism and premenstrual syndrome. The screening mechanisms may be used to measure the expression of the α4β2δ GABAA receptors, in order to identify members of the general population as having an increased sensitivity to lower concentrations alcohol coupled with a decrease sensitivity to higher concentrations of alcohol, a scenario frequently found in patients suffering from alcoholism and premenstrual anxiety. Methods of screening for drugs which decrease expression of the α4β2δ subunit of GABAA are also provided.

    摘要翻译： 本发明涉及用于鉴定酗酒和经前期综合征风险增加的一般群体成员的筛选机制。 筛选机制可以用于测量α4 N 2β2 GABA A A受体的表达，以便鉴定一般的成员 人群对低浓度酒精具有增加的敏感性，同时降低对较高浓度酒精的敏感性，这是在患有酒精中毒和经前期焦虑的患者中频繁发现的情况。 还提供了筛选降低GABA Aα的α4AT2AT2亚基的表达的药物的方法。

    Detection of cellular exposure to ethanol
    4.
    发明授权
    Detection of cellular exposure to ethanol 失效
    检测细胞暴露于乙醇

    公开(公告)号：US06255057B1

    公开(公告)日：2001-07-03

    申请号：US08686796

    申请日：1996-07-26

    申请人： Adrienne Sue Gordon ,  Ivan Diamond ,  Doug Paul Dohrman

    发明人： Adrienne Sue Gordon ,  Ivan Diamond ,  Doug Paul Dohrman

    IPC分类号： G01N3353

    CPC分类号： G01N33/573 ,  G01N33/98 ,  G01N2800/307

    摘要： The present invention relates to determinable effects of ethanol exposure on the cellular localization and abundance of specific proteins, referred to herein as ethanol indicative proteins. More specifically, the present invention is based, in part, on the discovery that the catalytic C&agr; subunit of cAMP dependent protein kinase (PKA), which is normally localized in the Golgi apparatus area, appears to translocate to the nucleus upon exposure of a cell to ethanol. The present invention is further based on the observation that the &dgr;-subunit of PKC translocates from the Golgi area to the perinucleus and the nucleus in response to ethanol exposure, while the &egr;-subunit of PKC migrates from the perinucleus into the cytoplasm. The present invention further relates to the discovery that the detectable amount of the regulatory subunit RI of PKA decreases, while the detectable amount of &agr;PKC, &dgr;PKC and &egr;PKC increases upon exposure of a cell to ethanol. These discoveries provide the basis for assays that may be used to detect the exposure of cells to ethanol and assays that may be used for the screening of drugs or the development of treatments to modulate the effects of ethanol consumption. The invention further relates to kits for detecting the exposure of cells to ethanol. Kits of the invention may include antibodies, which preferably are labeled, capable of specifically binding to C&agr; or RI of the cAMP-dependent protein kinase, or, capable of specifically binding to &agr;PKC, &dgr;PKC or &egr;PKC.

    摘要翻译： 本发明涉及乙醇暴露对细胞定位和特异性蛋白质丰度的可确定的影响，本文称为乙醇指示蛋白。 更具体地，本发明部分地基于以下发现：通常位于高尔基体区域中的cAMP依赖性蛋白激酶（PKA）的催化Calpha亚基似乎在细胞暴露时易位于细胞核 到乙醇。 本发明进一步基于以下观察：PKC的δ-亚基从高尔基域转移到响应于乙醇暴露的周核和核，而PKC的ε亚单位从周核迁移到细胞质中。 本发明还涉及PKA的调节亚基RI的可检测量减少的发现，而当细胞暴露于乙醇时，可检测量的αPKC，δPKC和epsiPKC增加。 这些发现提供了可用于检测细胞暴露于乙醇的测定的基础，以及可用于筛选药物或开发用于调节乙醇消耗的影响的处理的测定。 本发明还涉及用于检测细胞暴露于乙醇的试剂盒。 本发明的试剂盒可以包括优选被标记的能够特异性结合cAMP依赖性蛋白激酶的Calpha或RI的抗体，或能够特异性结合αPKC，δPKC或epsiPKC的抗体。

    Plasma and hemoglobin-associated acetaldehyde as a marker of alcohol use
    6.
    发明授权
    Plasma and hemoglobin-associated acetaldehyde as a marker of alcohol use 失效
    血浆和血红蛋白相关乙醛作为酒精使用标志物

    公开(公告)号：US4814280A

    公开(公告)日：1989-03-21

    申请号：US82518

    申请日：1987-08-07

    申请人： Charles M. Peterson

    发明人： Charles M. Peterson

    IPC分类号： G01N33/52 ,  G01N33/72 ,  G01N33/98 ,  G01N33/48

    CPC分类号： G01N33/721 ,  G01N33/52 ,  G01N33/98 ,  G01N2800/307 ,  Y10T436/200833 ,  Y10T436/204165

    摘要： An improved fluorigenic, high-performance, liquid chromatography (HPLC) assay comprises reacting plasma and hemolysate samples with cyclohexanedione in the presence of ammonium ion to form a methylacridine dione fluorigenic species that is stable and exhibits high recovery values. Acetaldehyde content was determined by comparing the fluorescent peaks of the HPLC eluted band with standard peaks. Alcohol intake by the subject was detected by comparing the acetaldehyde levels to those of teetotalers.

    摘要翻译： 改进的荧光，高性能，液相色谱（HPLC）测定法包括在铵离子存在下使血浆和溶血样品与环己二酮反应，以形成稳定且显示高回收率的甲基吖啶二酮荧光物质。 通过比较HPLC洗脱带的荧光峰与标准峰确定乙醛含量。 通过比较乙醛水平与乙醇水平相比，检测到受试者的酒精摄入量。

    SEROTONIN TRANSPORTER GENE AND TREATMENT OF ALCOHOLISM
    10.
    发明申请
    SEROTONIN TRANSPORTER GENE AND TREATMENT OF ALCOHOLISM 审中-公开
    丝氨酸运输基因和酒精处理

    公开(公告)号：US20160376658A1

    公开(公告)日：2016-12-29

    申请号：US15243682

    申请日：2016-08-22

    申请人： University of Virginia Patent Foundation

    发明人： Bankole A. Johnson

    IPC分类号： C12Q1/68 ,  G01N33/94

    CPC分类号： C12Q1/6883 ,  A61K31/4178 ,  A61K45/06 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/6872 ,  G01N33/942 ,  G01N2800/307 ,  G01N2800/50

    摘要： The gene responsible for encoding SERT has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3′ UTR SNP rs1042173. The present invention demonstrates the efficacy of using the drug ondansetron and similar drugs for treatment based on variations in the polymorphisms of the SERT gene as well as methods for diagnosing susceptibility to abuse of alcohol and other addiction-related diseases and disorders.

    摘要翻译： 负责编码SERT的基因在5'调节启动子区具有功能多态性，其形成长（L）和短（S）两种形式。 假设LL型基因型在酒精使用早期发挥关键作用。 本发明公开了基于L或短基因型的治疗和诊断的差异以及SERT基因，3'UTR SNP rs1042173的单核苷酸多态性。 本发明证明了基于SERT基因多态性变化的药物昂丹司琼和类似药物治疗的疗效以及用于诊断酒精和其他成瘾相关疾病和病症滥用易感性的方法。