公开(公告)号：US12233188B2

公开(公告)日：2025-02-25

申请号：US17569738

申请日：2022-01-06

Applicant: Regenexx, LLC

Inventor： Christopher Centeno ,  Ryan Dregalla ,  Nicolette Lyons ,  Patrick Reischling

IPC: A61M1/02 ,  A61K35/28 ,  A61M1/36 ,  B01D21/26 ,  B01L3/00 ,  B04B7/08 ,  C12M1/00 ,  C12M1/26 ,  C12N5/077 ,  C12N5/0775 ,  G01N33/49 ,  G01N33/68

Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems, devices, and methods of obtaining, utilizing, and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.

公开(公告)号：US12233091B2

公开(公告)日：2025-02-25

申请号：US16862069

申请日：2020-04-29

Applicant: Mirror Biologics, Inc.

Inventor： Michael Har-Noy

IPC: A61K35/28 ,  A61K35/12 ,  A61K39/00 ,  A61K39/39 ,  C12N5/0783

Abstract: The present invention relates to pharmaceutical vaccine compositions comprising at least one vaccine antigen together with living immune cells. These immune cells include at least a portion of activated T-cells and act as an adjuvant. Methods for using these pharmaceutical compositions to prevent or treat diseases, such as cancer, infectious diseases and autoimmune disease are also included.

公开(公告)号：US12226436B2

公开(公告)日：2025-02-18

申请号：US16071123

申请日：2017-01-18

Applicant: ROHTO PHARMACEUTICAL CO., LTD

Inventor： Daisuke Kajita ,  Satsuki Fukushima ,  Shigeru Miyagawa ,  Yoshiki Sawa

IPC: A61K35/28 ,  A61K35/35 ,  A61K35/51 ,  A61K35/545

Abstract: It has been found that a cell cluster obtained by causing just isolated cells to adhere to each other secretes adiponectin after transplantation to the heart, and thereby has an excellent therapeutic effect on heart disease.

公开(公告)号：US20250041349A1

公开(公告)日：2025-02-06

申请号：US18929289

申请日：2024-10-28

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Ewelina Morawa ,  Tirtha Chakraborty ,  Ante Sven Lundberg ,  Tony Ho ,  Laura Sandler ,  Brenda Eustace ,  Jerome Rossert ,  Robert Kauffman

IPC: A61K35/28 ,  A61K31/255 ,  A61K31/395 ,  A61K35/18 ,  A61K38/19 ,  A61P7/00 ,  C12N5/0789 ,  C12N9/22 ,  C12N15/11

Abstract: Provided herein, in some embodiments, are methods and compositions for treatment of subjects with β-thalassemia and subjects with severe sickle cell disease using autologous CRISPR-Cas9 modified CD34+ human hematopoietic stem and progenitor cells.

公开(公告)号：US20250041348A1

公开(公告)日：2025-02-06

申请号：US18792104

申请日：2024-08-01

Applicant: Wisconsin Alumni Research Foundation

Inventor： Peiman Hematti ,  Connie Chamberlain ,  John Kink ,  Ray Vanderby ,  Brian Walczak

IPC: A61K35/28 ,  A61P19/04 ,  C12N5/0775

Abstract: An ex vivo generated population of tissue-specific extracellular exosomes and methods of making and using such exosomes for treating orthopedic injury are provided.

公开(公告)号：US20250041347A1

公开(公告)日：2025-02-06

申请号：US18662450

申请日：2024-05-13

Applicant: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

Inventor： Jonathan ALEXANDER ,  Mansoureh BARZEGAR ,  Yuping WANG

IPC: A61K35/28 ,  A61K9/00 ,  A61K45/06 ,  A61P9/10

Abstract: Therapeutics and methods of treating a stroke in a patient comprising delivering stem cells into a peritoneum of the patent. According to a further embodiment the stem cells are mesenchymal stem cells. According to a further embodiment the stem cells are human placenta mesenchymal stem cells (hPMSC). According to a further embodiment the stroke is one of stroke is one of occlusive, post-occlusive, hemorrhagic, and transient ischemic injury. According to a further embodiment the method further comprises the step of delivering a clot busting compound into a blood stream of the patient.

公开(公告)号：US12214093B2

公开(公告)日：2025-02-04

申请号：US16227094

申请日：2018-12-20

Applicant: SEXTON BIOTECHNOLOGIES, INC.

Inventor： Steven Charlebois ,  Sreedhar Thirumala

IPC: A61L2/00 ,  A61K35/19 ,  A61K35/28 ,  A61P37/00 ,  C12N5/0775 ,  C12N5/078

Abstract: Disclosed are methods for treating platelet compositions (e.g. platelet concentrates and/or platelet lysates) with electron beam radiation, where the compositions are in a frozen state during irradiation with the e-beam radiation. The methods can be conducted using e-beam radiation at doses effective to reduce the pathogen content of the compositions while retaining highly beneficial bioactivities of the compositions. Also disclosed are compositions preparable by the methods, and methods and compositions involving the use of the e-beam treated materials.

公开(公告)号：US20250032551A1

公开(公告)日：2025-01-30

申请号：US18717354

申请日：2022-11-25

Applicant: Kyoto University

Inventor： Makoto IKEYA ,  Daisuke KAMIYA

IPC: A61K35/28 ,  A61P21/00 ,  C12N5/0775

Abstract: The present invention provides a method for producing, from pluripotent stem cells, neural crest cells specialized for differentiation into a mesenchymal lineage, wherein the method includes: 1) a step for culturing pluripotent stem cells under xeno-free and feeder-free conditions in culture broth containing an ALK inhibitor and a GSK-3β inhibitor to obtain neural crest cells; and 2) a step for culturing the neural crest cells under xeno-free and feeder-free conditions in culture broth that includes an ALK inhibitor, EGF, and FGF and is substantially free of GSK-3β inhibitor.

公开(公告)号：US20250032549A1

公开(公告)日：2025-01-30

申请号：US18600407

申请日：2024-03-08

Applicant: Ensoma, Inc.

Inventor： Dwight Morrow ,  Patrick C. Falahee ,  Anthony Boitano ,  Michael P. Cooke ,  Kevin A. Goncalves

IPC: A61K35/28 ,  A61K31/4427 ,  A61K35/12 ,  A61K38/19 ,  A61K45/06 ,  A61P37/00 ,  C07K14/52 ,  C12N5/0789

Abstract: The invention provides compositions and methods useful for mobilizing populations of hematopoietic stem and progenitor cells within a donor, as well as for determining whether samples of mobilized cells are suitable for release for ex vivo expansion and/or therapeutic use. In accordance with the compositions and methods described herein, mobilized hematopoietic stem and progenitor cells can be withdrawn from a donor and administered to a patient for the treatment of various stem cell disorders, including hematopoietic diseases, metabolic disorders, cancers, and autoimmune diseases, among others. In certain embodiments, the compositions and methods described herein lead to the mobilization of a population of CD34dim cells that have immunosuppressive effects and that can reduce the incidence of graft vs. host disease.

公开(公告)号：US20250032405A1

公开(公告)日：2025-01-30

申请号：US18696894

申请日：2022-09-28

Applicant: CELLSREGEN INC.

Inventor： David R. ELMALEH ,  Aram AKBARZADEH ,  Minh Khanh NGUYEN

IPC: A61K9/00 ,  A61K9/06 ,  A61K35/28 ,  A61K39/00 ,  A61K47/42

Abstract: This invention relates to producing vaccines, such as cancer vaccines, by methods of forming an expansion of a lymphoid network in a subject the method comprising: administering to the subject a polymer suspension composition comprising: a natural polymer and cells; gelling the polymer suspension composition in vivo to form a hydrogel composition scaffold; and expanding the cell population to form an expansion of the lymphoid network, including in vivo. It also relates to hydrogel compositions comprising a natural polymer, along with cells, a pharmaceutical composition or a pharmaceutically acceptable salt thereof, a modulating agent, and/or a targeting agent, as well as methods of making and using the hydrogel compositions as biotherapeutic scaffolds for treatment of cancers or tumors, including in vivo.