公开(公告)号：US20250052727A1

公开(公告)日：2025-02-13

申请号：US18933343

申请日：2024-10-31

Applicant: Waters Technologies Corporation

Inventor： Edouard S. P. Bouvier ,  Kevin Wyndham ,  Thomas H. Walter ,  Uwe D. Neue

IPC: G01N30/02 ,  B01D15/34 ,  B01J20/28 ,  B01J20/282 ,  B01J20/286 ,  G01N21/53

Abstract: The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles.

公开(公告)号：US20250041760A1

公开(公告)日：2025-02-06

申请号：US18362691

申请日：2023-07-31

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Ta-Chen WEI ,  Andrei BORDUNOV ,  Charu KUMAR ,  Ace Gita GALERMO ,  John DAVIES ,  Andrew COFFEY

IPC: B01D15/20 ,  B01D15/34 ,  B01J20/28 ,  B01J20/283

Abstract: A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.

公开(公告)号：US12110312B2

公开(公告)日：2024-10-08

申请号：US16908401

申请日：2020-06-22

Applicant: Genzyme Corporation

Inventor： Konstantin Konstantinov ,  Rahul Godawat ,  Veena Warikoo ,  Sujit Jain

IPC: B01D15/18 ,  B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  B01D15/42 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00 ,  C12M1/00 ,  C12N9/00 ,  C12N9/24 ,  C12N9/40 ,  C12P21/00

CPC classification number: C07K1/22 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/1871 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3814 ,  B01D15/422 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C12M23/52 ,  C12M47/10 ,  C12M47/12 ,  C12N9/00 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/00 ,  C12P21/005 ,  C12Y302/01022 ,  C12Y302/01045 ,  C07K2317/14

Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.

公开(公告)号：US20240254160A1

公开(公告)日：2024-08-01

申请号：US18629465

申请日：2024-04-08

Applicant: Kashiv BioSciences, LLC

Inventor： Roshan Ganeshlal Upadhyay ,  Darshana Jiten Maniar ,  Shivani Singh

IPC: C07K1/16 ,  B01D15/34 ,  B01J20/28 ,  B01J20/283 ,  C07K14/705

CPC classification number: C07K1/16 ,  B01D15/34 ,  B01J20/28083 ,  B01J20/283 ,  C07K14/70521 ,  C07K2319/30

Abstract: The present invention provides an effective High Performance Liquid Chromatography (SE-HPLC) method to separate or resolve the pre-peak, post-peak and main peak of CTLA4-IgG1 fusion protein, where pre-peak resolution is more than 1.3. The invention further provides effective separation and resolution of post peak impurity present in CTLA4 IgG1 protein. Moreover, the present invention also provides the method for the estimation and/or quantification of pre-peak, post-peak and main peak of the protein mixture.

公开(公告)号：US20240128067A1

公开(公告)日：2024-04-18

申请号：US18529686

申请日：2023-12-05

Applicant: POLYMER FACTORY SWEDEN AB

Inventor： Scott M. GRAYSON

IPC: H01J49/00 ,  B01D15/34 ,  C07C69/30 ,  C07C69/33 ,  C07C69/67 ,  G01N27/623 ,  G01N30/04

CPC classification number: H01J49/0009 ,  B01D15/34 ,  C07C69/30 ,  C07C69/33 ,  C07C69/67 ,  G01N27/623 ,  G01N30/04 ,  G01N2030/042

Abstract: Calibrants and methods of making the same include using mixtures of multifunctional core compounds or multifunctional dendrimers that are functionalized to yield a set of discrete compounds which cover a range of collisional cross sections (CCSs) for calibrating ion mobility and variation in molecular weight to calibrate the mass to charge (m/z) measurements of mass spectrometry, as well as for calibrating tandem instruments that measure both dimensions. Methods of using the calibrants are also disclosed, such as in mass spectrometry.

公开(公告)号：US11862446B2

公开(公告)日：2024-01-02

申请号：US16617692

申请日：2018-05-29

Applicant: The Administrators of the Tulane Educational Fund

Inventor： Scott M. Grayson

IPC: H01J49/00 ,  B01D15/34 ,  C07C69/30 ,  C07C69/33 ,  C07C69/67 ,  G01N30/04 ,  G01N27/623

CPC classification number: H01J49/0009 ,  B01D15/34 ,  C07C69/30 ,  C07C69/33 ,  C07C69/67 ,  G01N27/623 ,  G01N30/04 ,  G01N2030/042

Abstract: Calibrants and methods of making the same include using mixtures of multifunctional core compounds or multifunctional dendrimers that are functionalized to yield a set of discrete compounds which cover a range of collisional cross sections (CCSs) for calibrating ion mobility and variation in molecular weight to calibrate the mass to charge (m/z) measurements of mass spectrometry, as well as for calibrating tandem instruments that measure both dimensions. Methods of using the calibrants are also disclosed, such as in mass spectrometry.

公开(公告)号：US11826724B2

公开(公告)日：2023-11-28

申请号：US17372985

申请日：2021-07-12

Applicant: CytoSorbents Corporation

Inventor： Tamaz Guliashvili ,  Thomas Golobish ,  Maryann Gruda ,  Pamela O'Sullivan ,  Andrew Scheirer ,  Vincent Capponi ,  Phillip Chan ,  Wei-Tai Young

IPC: A61K38/00 ,  B01J20/26 ,  B01D15/34 ,  B01J20/28 ,  B01J20/32 ,  A61K9/00 ,  A61K31/745 ,  A61M1/36 ,  B01D15/12

CPC classification number: B01J20/261 ,  A61K9/0031 ,  A61K9/0053 ,  A61K31/745 ,  A61M1/3679 ,  B01D15/34 ,  B01J20/262 ,  B01J20/267 ,  B01J20/2808 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/327 ,  B01J20/3208 ,  B01J20/3231 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3289 ,  B01D15/125

Abstract: The invention concerns biocompatible polymer systems comprising at least one polymer with a plurality of pores, said polymer comprising either polyol or zwitterionic groups designed to adsorb endotoxins and other inflammatory mediator molecules. The inventions are in the field of porous polymeric sorbents, also in the field of broadly reducing endotoxins in blood and blood products that can cause endotoxemia, additionally, in the field of broadly removing endotoxins by perfusion or hemoperfusion.

公开(公告)号：US20230028028A1

公开(公告)日：2023-01-26

申请号：US17777338

申请日：2020-11-24

Applicant: Fujifilm Manufacturing Europe B.V. ,  Fujifilm Corporation

Inventor： Berry Bogels ,  Yujiro Itami

IPC: B01D67/00 ,  C07K1/22 ,  C12N15/10 ,  B01D69/10 ,  B01D69/02 ,  B01D15/34 ,  B01D15/36 ,  B01D61/14

Abstract: A porous membrane obtainable by a process comprising curing a composition comprising: (i) cross-linking agent(s) comprising at least one ligand group; (ii) inert solvent(s); (iii) polymerization initiator(s); and (vi) optionally monomer(s) other than component (i) which are reactive with component (i); wherein the composition satisfies the following equation: Z=wt(i)/(wt(i)+wt(iii)+wt(iv)) wherein: Z has a value of at least 0.6; wt(i) is the number of grammes of component (i) present in the composition; wt(iii) is the number of grammes of component (iii) present in the composition; and wt(iv) is the number of grammes of component (iv) present in the composition.

公开(公告)号：US20230027693A1

公开(公告)日：2023-01-26

申请号：US17951674

申请日：2022-09-23

Applicant: KBIO HOLDINGS LIMITED

Inventor： Leigh Burden ,  Steven D. Hume ,  Joshua Morton ,  Greg Pogue ,  Barry Bratcher ,  Hugh A. Haydon ,  Carrie A. Simpson ,  Nick Partain ,  John W. Shepherd

IPC: C12N7/00 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  A61K47/69 ,  A61K38/36 ,  A61K39/00 ,  A61K39/145 ,  C07K1/18 ,  C07K1/16 ,  C07K1/22

Abstract: Disclosed herein are methods and exemplary compositions associated with virus purification, antigen purification, and conjugation of virus and proteins (e.g., antigen) to form vaccines for delivery of immunological and other therapeutic agents, exemplary aspects of which may include harvesting viral and antigenic substances from source organisms; a purification platform comprising chemical separation and size-difference separation for the removal of contaminants, debris and impurities from the viral and protein (e.g. antigenic, including influenza hemagglutinin antigens) substances, as well as their concentration and collection; and a conjugation platform providing activation of the virus at a pH that increases binding rate and binding propensity between the virus and the protein, wherein embodiments related to the conjugation platform include controlling the ratio of virus to protein.

公开(公告)号：US20230002445A1

公开(公告)日：2023-01-05

申请号：US17853807

申请日：2022-06-29

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Tao Xing ,  Yuetian Yan ,  Shunhai Wang

IPC: C07K1/36 ,  C07K1/28 ,  C07K1/22 ,  G01N33/68 ,  B01D15/34 ,  B01D15/16 ,  G01N30/72

Abstract: The present invention generally pertains to methods of characterizing charge variants of a protein of interest. In particular, the present invention pertains to the use of desalting size exclusion chromatography-reduced peptide mapping mass spectrometry to identify charge variants separated by capillary isoelectric focusing.