DNA sequencing method
    2.
    发明授权

    公开(公告)号:US12129520B2

    公开(公告)日:2024-10-29

    申请号:US16327535

    申请日:2017-10-30

    申请人: PEKING UNIVERSITY

    摘要: Disclosed is a DNA sequencing method. The DNA sequencing method of this invention comprises: (1) adding a tag sequence at the 3′ terminus of the DNA to be sequenced so as to form a DNA to be sequenced including the tag sequence, the nucleotide sequence of said tag sequence being the reverse complement of the nucleotide sequence of the sequencing primer; (2) mixing the DNA to be sequenced including the tag sequence and the sequencing primer so as to form a product having a 5′ terminus double strand and a single main strand; (3) after step (2) is completed, the product is mixed separately with dATP, dCTP, dTTP, and dGTP to obtain four systems, each system is separately added to a single-molecule device modified by a DNA polymerase, and electric signals are read. Experiments verify that the method of this invention performs DNA sequencing and has important application value.