Cellular transport system for transferring a sulfonic acid construct carrying a cargo into the cytoplasm of a cell

    公开(公告)号:US11661398B2

    公开(公告)日:2023-05-30

    申请号:US16652949

    申请日:2018-10-02

    IPC分类号: C07C309/17 C12N9/10

    摘要: The present invention relates to a cellular transport system for bringing a sulfonic acid construct which carries a cargo into a cell and releasing the cargo in the cell's cytoplasm, the cellular transport system comprising: (i) a sulfonate transporter located in the cytoplasm membrane of the cell wherein said sulfonate transporter is capable of transporting said sulfonic acid construct across the cytoplasm membrane into the cytoplasm; (ii) a γ-glutamyl transferase (GGT; EC 2.3.2.2) which is modified to be located in the cytoplasm of the cell, wherein said γ-glutamyl transferase is capable of hydrolyzing said sulfonic acid construct so as to release the cargo. Moreover, the present invention relates to the use of a cellular transport system for bringing a sulfonic acid construct which contains a cargo into a cell and releasing the cargo in the cell's cytoplasm. Further, the present invention relates to a γ-glutamyl transferase for hydrolyzing a sulfonic acid construct which contains a cargo.

    RADIATION-SENSITIVE RESIN COMPOSITION, ONIUM SALT COMPOUND AND METHOD FOR FORMING RESIST PATTERN

    公开(公告)号:US20190285983A1

    公开(公告)日:2019-09-19

    申请号:US16424986

    申请日:2019-05-29

    申请人: JSR CORPORATION

    摘要: A radiation-sensitive resin composition includes a resin including a structural unit having an acid-dissociable group, an onium salt compound represented by formula (1), and a solvent.R1 is a hydrogen atom or a monovalent group provided that the monovalent group is not a fluoro group or a monovalent organic group containing a fluorine atom. X1 and X2 are each independently a single bond, —O—, —S— or —NR′— wherein R′ is a hydrogen atom or a monovalent hydrocarbon group. In a case where X1 is —NR′—, R2 is a monovalent organic group or a hydrogen atom. In a case where X2 is —NR′—, R3 is a monovalent organic group or a hydrogen atom. In a case where neither X1 nor X2 is —NR′—, R2 and R3 are each independently a monovalent organic group.