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公开(公告)号：US12193467B2
公开(公告)日：2025-01-14
申请号：US18656578
申请日：2024-05-06
Applicant: ZHEJIANG HUAKANG PHARMACEUTICAL CO., LTD.
Inventor： Baoxing Mao , Ling Wan , Jingjing Xu , Zhihui Yao , Bo Xu , Hang Yue , Fuan Wan
Abstract: A method for preventing crystal maltitol from caking in storage, comprising: detecting a temperature and a humidity of a dried crystal maltitol on-line in real time, an on-line temperature and humidity meter being configured to set a sieving and packaging treatment condition, a redrying treatment condition, and an aging treatment condition; performing a sieving and packaging treatment on the crystal maltitol whose temperature and humidity satisfy the sieving and packaging treatment condition, performing a redrying treatment or an aging treatment on the crystal maltitol whose temperature and humidity satisfy the redrying treatment condition or the aging treatment condition; detecting a temperature and a humidity of the crystal maltitol after the redrying treatment or the aging treatment, and performing the sieving and packaging treatment on the crystal maltitol whose temperature and humidity satisfy the sieving and packaging treatment condition, or else continuing with the redrying treatment or the aging treatment.
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公开(公告)号：US20240368207A1
公开(公告)日：2024-11-07
申请号：US18522146
申请日：2023-11-28
Applicant: WAVE LIFE SCIENCES LTD.
Inventor： David Charles Donnell Butler , Pachamuthu Kandasamy , Subramanian Marappan , Ik-Hyeon Paik , Jayakanthan Kumarasamy , Gopal Reddy Bommineni
IPC: C07H1/06 , C07H19/10 , C07H19/207 , C07H21/00
Abstract: The present disclosure, among other things, provides technologies for oligonucleotide synthesis. In some embodiments, the present disclosure provides phosphoramidites and methods for synthesis thereof. In some embodiments, provided methods provides higher yields and/or purities. In some embodiments, provided methods remove byproducts without contact with an aqueous solution.
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公开(公告)号：US12123040B2
公开(公告)日：2024-10-22
申请号：US17814337
申请日：2022-07-22
Applicant: Chr. Hansen HMO GmbH
Inventor： Stefan Jennewein
IPC: C12P19/26 , C07H1/00 , C07H1/06 , C12N1/20 , C12N15/72 , C12P19/00 , C12P19/12 , C12P19/14 , C12R1/19
CPC classification number: C12P19/26 , C07H1/00 , C07H1/06 , C12N1/205 , C12N15/72 , C12P19/00 , C12P19/12 , C12P19/14 , C12Y302/01 , C12Y302/01023 , C12N2800/101 , C12R2001/19
Abstract: The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.
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公开(公告)号：US12065462B2
公开(公告)日：2024-08-20
申请号：US16626217
申请日：2018-06-27
Applicant: GLYCOM A/S
Inventor： Nikolay Khanzhin
IPC: C07H1/06 , A23L33/00 , B01D61/02 , B01D61/04 , B01D61/14 , B01D61/58 , B01D69/02 , B01D69/12 , B01D71/56 , B01D71/58 , C07H3/06 , C08B37/00 , C12P19/04 , C12P19/12 , C12P19/18
CPC classification number: C07H1/06 , A23L33/40 , B01D61/027 , B01D61/04 , B01D61/146 , B01D61/58 , B01D69/02 , B01D69/12 , B01D71/56 , B01D71/58 , C07H3/06 , C08B37/0003 , C12P19/04 , C12P19/12 , C12P19/18 , A23L33/00 , A23V2002/00 , B01D61/145 , B01D2315/16 , B01D2325/20
Abstract: The invention relates to a method for separating sialylated oligosaccharides, preferably sialylated human milk oligosaccharides (HMOs), from disaccharides, preferably lactose, produced by a fermentation or enzymatic process.
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公开(公告)号：US12049476B2
公开(公告)日：2024-07-30
申请号：US18320823
申请日：2023-05-19
Applicant: SANIFIT THERAPEUTICS, S.A.
Abstract: The present invention provides processes for preparing soluble salts of inositol phosphate characterized by a low level of impurities. Processes for preparing soluble salts (e.g., alkali-metal or ammonium) of inositol phosphates (e.g., hexasodium or dodecasodium salts), and of inositol hexaphosphate in particular (e.g., Na6IP6 and Na12IP6), are described. Also provided are pharmaceutical compositions, methods of use, combination treatments, kits, and articles of manufacture comprising soluble salts of inositol phosphates prepared according to the method of the invention.
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公开(公告)号：US12043642B2
公开(公告)日：2024-07-23
申请号：US17236501
申请日：2021-04-21
Applicant: Hoffmann-La Roche Inc.
Inventor： Joerg Lill , Filippo Sladojevich
CPC classification number: C07H1/06 , B01D69/02 , C07H21/00 , B01D2325/20
Abstract: The invention relates to a new process for the purification of oligonucleotides which comprises the removal of the acid labile 5′hydroxy protecting group at the 5′-O-oligonucleotide terminus of the oligonucleotide by way of tangential flow filtration with an acidic buffer solution. The process requires less steps and allows a higher degree of automation.
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公开(公告)号：US20240034747A1
公开(公告)日：2024-02-01
申请号：US18377942
申请日：2023-10-09
Applicant: QINGDAO RESTORE BIOTECHNOLOGY CO., LTD.
Inventor： Mengmeng Chen , Bingqiang Zhang , Yu Zhang , Yi Zhao , Yuan Zhang , Gaofeng Zhang , Xueqi Fu , Wei Zou
IPC: C07H19/048 , C07H1/06 , C12P19/30
CPC classification number: C07H19/048 , C07H1/06 , C12P19/30
Abstract: The present invention belongs to the technical field of NMN preparation, and particularly relates to a recrystallization method for NMN preparation, comprising the following steps: step 1. preparation of raw materials: preparing nicotinamide, nucleic acid, ribose-5-phosphate and phosphoric acid; step 2. preparation of reaction solution: preparing a suitable buffer, dissolving nicotinamide, ribose, ribose-5-phosphate in the buffer, and adjusting the concentration of the solution; step 3. activated phosphorylation reaction: adding phosphoric acid to the buffer of the dissolved nicotinamide and ribose, and stirring the mixture at appropriate temperature and pH conditions to make reactions take place in the mixture; step 4. addition of enzyme for catalysis; step 5. separation and purification: separating and purifying the reacted mixture to remove unreacted substrates and synthetase. The present invention is convenient for improving the crystal purity of NMN and improving the crystal quality, and is convenient for users to prepare NMN crystals.
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公开(公告)号：US11884692B2
公开(公告)日：2024-01-30
申请号：US17811022
申请日：2022-07-06
Applicant: Translate Bio, Inc.
Inventor： Frank DeRosa , Anusha Dias , Michael Heartlein , Shrirang Karve
CPC classification number: C07H1/06 , C07H21/00 , C07H21/02 , C12N15/1017
Abstract: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.
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公开(公告)号：US20240026403A1
公开(公告)日：2024-01-25
申请号：US18344244
申请日：2023-06-29
Applicant: Chr. Hansen HMO GmbH
Inventor： Markus Helfrich , Stefan Jennewein
IPC: C12P19/02 , A23L33/125 , A23L33/00 , A61K31/7012 , C12P19/26 , C07H1/08 , C07H3/08 , C07H1/06 , C07H7/027
CPC classification number: C12P19/02 , A23L33/125 , A23L33/40 , A61K31/7012 , C12P19/26 , C07H1/08 , C07H3/08 , C07H1/06 , C07H7/027
Abstract: The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.
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公开(公告)号：US11834691B2
公开(公告)日：2023-12-05
申请号：US16765407
申请日：2018-10-31
Applicant: Chr. Hansen HMO GmbH
Inventor： Stefan Jennewein , Markus Helfrich
IPC: C12P19/02 , C07H1/06 , C07H7/027 , A23L33/125 , A23L33/00 , A61K31/7012 , C12P19/26 , C07H1/08 , C07H3/08
CPC classification number: C12P19/02 , A23L33/125 , A23L33/40 , A61K31/7012 , C07H1/06 , C07H1/08 , C07H3/08 , C07H7/027 , C12P19/26
Abstract: The present invention describes an efficient way to isolate a sialic acid from a fermentation broth. The sialic acid contained in the fermentation broth is produced by bacterial fermentation. The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide the sialic acid in spray-dried form as well as in form of sialic acid crystals.
