公开(公告)号：US20220363723A1

公开(公告)日：2022-11-17

申请号：US17767219

申请日：2020-10-27

申请人： National Research Council of Canada

发明人： Yves Durocher ,  Sylvie Perret ,  Simon Joubert

IPC分类号： C07K14/05 ,  C07K16/28

摘要： The present disclosure relates to the use of an Epstein Barr virus origin of replication (oriP) or a functional fragment thereof in a protein expression construct to increase production of a protein of interest in mammalian cells. Also disclosed are protein expression constructs for increased production of antibodies in mammalian cells, and mammalian cells containing the expression constructs.

公开(公告)号：US20210222182A1

公开(公告)日：2021-07-22

申请号：US17151162

申请日：2021-01-17

申请人： Walter W. Aller

发明人： Walter W. Aller

IPC分类号： C12N15/79 ,  C07K14/05 ,  C07K14/12

摘要： A method for treating cancer in mammals using an expression vector to transfect cancer cells in vivo and in situ. The vector includes one or more immunogenic exogenous polypeptides that are expressed by the cancer cells upon transfection. The body's immune response is triggered and directed to attack the transfected cancer cells. Once the immune response to the exogenous peptide on the cancer cells is initiated, an immune response to the other cancer associated or cancer specific antigens on or in the cancer cells takes over and eliminates all cancer cells, transfected or not.

公开(公告)号：US20210038711A1

公开(公告)日：2021-02-11

申请号：US16970084

申请日：2019-02-15

申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY

发明人： David Weiner ,  Alfredo Perales Puchalt

IPC分类号： A61K39/245 ,  A61P31/22 ,  C12N15/79 ,  C07K14/05

摘要： Nucleic acid molecules and compositions comprising one or more nucleotide sequences that encode a consensus Epstein-Barr virus (EBV) antigen. Immunomodulatory methods and methods of inducing an immune response against EBV are disclosed. Method of treating infection by EBV and methods of treating or preventing a disease or disorder associated with EBV are disclosed. Modified consensus EBV antigens are disclosed.

公开(公告)号：US10772914B1

公开(公告)日：2020-09-15

申请号：US16388776

申请日：2019-04-18

申请人： Baylor College of Medicine

发明人： Cliona M. Rooney ,  Sandhya Sharma

IPC分类号： A61K35/17 ,  C12N5/0783 ,  A61P35/00 ,  C07K14/05

摘要： Methods for generating/expanding populations of immune cells comprising immune cells specific for an Epstein Barr Virus (EBV) lytic antigen are disclosed, the methods comprising stimulating immune cells specific for an EBV lytic antigen by contacting peripheral blood mononuclear cells (PBMCs) with: (i) one or more peptides corresponding to all or part of one or more EBV lytic antigens; or (ii) antigen presenting cells (APCs) presenting one or more peptides corresponding to all or part of one or more EBV lytic antigens. Also disclosed are populations of immune cells comprising immune cells specific for an EBV lytic antigen expanded according to such methods, and uses thereof.

公开(公告)号：US10414802B2

公开(公告)日：2019-09-17

申请号：US15522455

申请日：2015-10-29

申请人： GLAXOSMITHKLINE BIOLOGICALS S.A.

发明人： Andrea Carfi ,  Claudio Ciferri ,  Irmgard Hofmann ,  Holger Laux ,  Anders Lilja ,  Yingxia Wen

IPC分类号： C07K14/045 ,  C07K14/005 ,  A61K39/00 ,  C12N5/071 ,  C07K14/05

摘要： This invention relates to cytomegalovirus (CMV) proteins suitable for vaccine uses. Provided herein are mammalian host cells, in particular CHO cells, in which the sequence(s) encoding CMV proteins gH, gL, pUL128, pUL130, pUL131 (or a complex-forming fragment thereof) are stably integrated into the genome.

公开(公告)号：US10377798B2

公开(公告)日：2019-08-13

申请号：US15100914

申请日：2014-12-02

申请人： The United States of America as Represented by the Secretary of the Department of Health and Human Services

发明人： Surender Khurana ,  Hana Golding

IPC分类号： C07K14/005 ,  A61K39/155 ,  C07K14/05 ,  C07K16/10 ,  C12N7/00 ,  A61K39/00

摘要： Compositions and methods useful for producing an immune response in a subject specific for the RSV G protein are described herein. The new methods and compositions described herein are made possible by the development of a new recombinant RSV G protein fragment, which has been engineered for in vitro production and is antigenically similar to the native RSV G protein. The recombinant RSV G protein fragment is capable of inducing the production of RSV G-specific antibodies when injected into a subject. These antibodies can recognize both RSV A and RSV B strains and inhibit infection of both viruses. Accordingly, the compositions and methods described herein may be useful in protecting subjects from RSV infection via immunization, raising antibodies specific for RSV, which can in turn be used to treat RSV infection.

公开(公告)号：US20110039339A1

公开(公告)日：2011-02-17

申请号：US12989898

申请日：2009-03-09

申请人： Yves Durocher ,  Martin Loignon

发明人： Yves Durocher ,  Martin Loignon

IPC分类号： C12N15/87 ,  C07K14/05 ,  C12N5/10 ,  C12N15/63 ,  C12N15/85 ,  C07H21/00

CPC分类号： C12N15/85 ,  C07K14/005 ,  C07K2319/71 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2800/40

摘要： Processes vectors and engineered cell lines for large-scale transfection and protein production in mammalian cells, especially Chinese Hamster Ovary (CHO) cells are described in which transfection efficiencies are realized through the use of a single vector system, the use of functional oriP sequences in all plasmids, the use of codon-optimized Epstein-Barr virus nuclear antigen-1 (EBNA1) constructs the use of a fusion protein between a truncated Epstein-Barr virus nuclear antigenen-1c (EBNA1c) protein and a herpes simplex virus protein VP16, the use of a 40 kDa fully deacetylated poly(ethylenimine) as a transfection reagent, the use of co-expression of a fibroblast growth factor (FGF) and/or the use of protein kinase B to potentiate heterologous gene expression enhancement by valproic acid (VPA).

摘要翻译： 描述了用于哺乳动物细胞，特别是中国仓鼠卵巢（CHO）细胞中大规模转染和蛋白质生产的载体和工程细胞系，其中转染效率通过使用单一载体系统实现，使用功能性oriP序列 所有质粒，使用密码子优化的爱泼斯坦 - 巴尔病毒核抗原-1（EBNA1）构建了截短的爱泼斯坦 - 巴尔病毒核抗原-1（EBNA1c）蛋白和单纯疱疹病毒蛋白VP16之间的融合蛋白， 使用40kDa完全脱乙酰化的聚（亚乙基亚胺）作为转染试剂，使用共表达成纤维细胞生长因子（FGF）和/或使用蛋白激酶B来加强丙戊酸的异源基因表达增强（ VPA）。

公开(公告)号：US07060283B1

公开(公告)日：2006-06-13

申请号：US08392934

申请日：1993-09-15

申请人： Richard S. Smith ,  Gary R. Pearson ,  D. Elliot Parks ,  Susan Pothen Varghese

发明人： Richard S. Smith ,  Gary R. Pearson ,  D. Elliot Parks ,  Susan Pothen Varghese

IPC分类号： C07K14/05 ,  C07K4/02 ,  C07K14/01

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2317/734 ,  C12N2710/16222 ,  C12N2710/16622 ,  Y10S435/975 ,  Y10S530/826

摘要： Epstein-Barr virus (EBV) specific polypeptides consisting of a series of one to 1000 peptide units selected from the group consisting of peptide units Φ, Γ, Δ and Ω, wherein Φ is 25 amino acids or less and has the formula (αETFTETWNRFITHTEβ) (SEQ ID NO:1), Γ is 25 amino acids or less and has the formula (αGMLEASEGLDGWIHQβ) (SEQ ID NO:2), Δ is 25 amino acids or less and has the formula (αHQQGGWSTLIEDNIβ) (SEQ ID NO:3), Ω is 25 amino acids or less and has the formula (αKQKHPKKVKQAFNPLβ) (SEQ ID NO:4), α and β are each independently from 0 to 5 naturally occurring amino acids, and the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease are disclosed. Also disclosed are the use of these polypeptides for the production of polypeptide-specific antibodies and the diagnosis and treatment of EBV-associated disease.

摘要翻译： 由一系列1至1000个肽单元组成的爱泼斯坦 - 巴尔病毒（EBV）特异性多肽，其选自肽单元Phi，Gamma，Delta和Omega，其中Phi为25个氨基酸或更少，具有式（αETFTETWNRFITHTEβ） （SEQ ID NO：1），Gamma为25个氨基酸以下，具有式（αGMLEASEGLDGWIHQbeta）（SEQ ID NO：2），Δa为25个氨基酸以下，具有式（αHQQGGWSTLIEDNIPbeta）（SEQ ID NO：3 ），Omega为25个氨基酸以下，具有式（αKQKHPKKVKQAFNPLbeta）（SEQ ID NO：4），α和β各自独立为0〜5个天然存在的氨基酸，并且该多肽能够结合样品中的抗体 来自患有爱泼斯坦 - 巴尔病毒（EBV）相关疾病的个体。 还公开了这些多肽用于产生多肽特异性抗体以及EBV相关疾病的诊断和治疗的用途。

公开(公告)号：US20060057563A1

公开(公告)日：2006-03-16

申请号：US11192070

申请日：2005-07-29

申请人： Manfred Motz ,  Georg Bauer ,  Erwin Soutschek

发明人： Manfred Motz ,  Georg Bauer ,  Erwin Soutschek

IPC分类号： C12Q1/70 ,  C07H21/04 ,  C07K14/05

CPC分类号： G01N33/56994

摘要： In the area of virus diagnosis, in particular Epstein-Barr virus (EBV) diagnosis, methods for the detection of EBV and agents suitable for this purpose are provided, including peptides which are derived from p18-VCA and permit discrimination between acute and past EBV infection.

公开(公告)号：US20050260564A1

公开(公告)日：2005-11-24

申请号：US10848976

申请日：2004-05-19

申请人： Bill Sugden ,  Jindong Wang ,  Gregory Kennedy

发明人： Bill Sugden ,  Jindong Wang ,  Gregory Kennedy

IPC分类号： A61K48/00 ,  C07K14/05 ,  C12N7/00 ,  C12N15/86 ,  C12N15/869 ,  C12Q1/70

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N2710/16222 ,  C12N2710/16243 ,  C12N2840/203

摘要： The invention provides a vector encoding a derivative of EBNA-1 that is not cytotoxic when expressed efficiently in cells, which supports extrachromosomal replication, maintenance and transcription from extrachromosomal oriP containing vectors but does not substantially activate transcription from host cell genes. Also provided is a vector having oriP and encoding a derivative of EBNA-1. The vectors of the invention may be employed in vitro and in gene therapy.

摘要翻译： 本发明提供了编码EBNA-1衍生物的载体，当在细胞中有效表达时，其不具有细胞毒性，其支持来自染色体外含oriP的载体的染色体外复制，维持和转录，但基本上不激活宿主细胞基因的转录。 还提供了具有oriP并编码EBNA-1的衍生物的载体。 本发明的载体可以在体外和基因治疗中使用。