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公开(公告)号:US20230124565A1
公开(公告)日:2023-04-20
申请号:US17800405
申请日:2021-02-05
发明人: Jae Young YANG , Ji Sung PARK , Jae Ho SHIM , Yoon Joeng UM , Moon Jae LEE
摘要: The present invention relates to a method of preparing a population of antibodies, whereby a desired high-purity and high-quality population of antibodies can be prepared by removing impurities without using an expensive protein A column, and in particular, production costs can be significantly reduced while achieving process automation; and a population of antibodies prepared thereby.
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公开(公告)号:US20230069095A1
公开(公告)日:2023-03-02
申请号:US17821835
申请日:2022-08-24
申请人: AMGEN INC.
发明人: Elaheh Binabaji , Chio Mui Chan , Hadley Krizner , Junfen Ma , Diana Woehle
摘要: The present disclosure relates to methods of re-oxidizing an antigen-binding protein.
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公开(公告)号:US20230064378A1
公开(公告)日:2023-03-02
申请号:US17817754
申请日:2022-08-05
发明人: Shane Krummen ATWELL , Yiqing FENG , Maya Rachel KARTA , Donmienne LEUNG , Songqing NA , Diana Isabel RUIZ , David John STOKELL , Laura Anne Pelletier
摘要: The present disclosure relates to antibodies that specifically bind human IL-4Rα, compositions comprising such IL-4Rα antibodies, and methods of using such IL-4Rα antibodies.
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公开(公告)号:US20230048658A1
公开(公告)日:2023-02-16
申请号:US17825793
申请日:2022-05-26
申请人: AMGEN INC.
摘要: This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.
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公开(公告)号:US11578098B2
公开(公告)日:2023-02-14
申请号:US16793676
申请日:2020-02-18
发明人: Glen Bolton , Keith Selvitelli
摘要: The present invention is directed to a method of inactivating virus that is present during production of a polypeptide of interest. In particular, the present invention is directed to a method of on-column virus inactivation using a low pH and high salt wash solution that effectively inactivates viruses with minimum recovery loss of the polypeptide.
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公开(公告)号:US20230042877A1
公开(公告)日:2023-02-09
申请号:US17835370
申请日:2022-06-08
发明人: Stephen Anderson , Elliot Campbell
摘要: The invention describes a method of generating antibodies to a mixture of peptidogenic proteins wherein the peptidogenic protein has altered conformational dynamics as compared to a starting protein and wherein the peptidogenic protein has a similar conformation to the starting protein. The peptidogenic proteins can be used to induce an immune response, which can lead to the generation of antibodies and/or can be used to vaccinate a mammal.
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公开(公告)号:US11566082B2
公开(公告)日:2023-01-31
申请号:US15525688
申请日:2015-11-11
发明人: Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC分类号: C07K16/46 , B01D15/38 , B01J20/289 , B01J20/32 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/06
摘要: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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公开(公告)号:US11566074B2
公开(公告)日:2023-01-31
申请号:US15764041
申请日:2016-09-29
发明人: Hiroyuki Ebinuma , Kengo Fujimura
IPC分类号: C07K16/24 , C07K16/28 , C12N5/12 , G01N33/543 , G01N33/577 , C07K14/715 , C12N15/02 , C12P21/00 , G01N33/53 , C07K16/06 , G01N33/68
摘要: Provided are a method of efficiently producing an sIL-2R antigen in an amount necessary for antibody generation, and a method of producing an anti-sIL-2R antibody using the antigen. Specifically, provided are a method of producing soluble interleukin-2 receptor, including culturing SCC-3 cells and recovering soluble interleukin-2 receptor from a culture of the cells, and a method of producing an anti-soluble interleukin-2 receptor antibody, including immunizing an animal with sIL-2R produced by the method.
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公开(公告)号:US11555052B2
公开(公告)日:2023-01-17
申请号:US16648014
申请日:2018-09-26
申请人: BAYER HEALTHCARE LLC
发明人: Kathryn Kwant
摘要: This disclosure relates to methods for the prevention of the reduction of disulfide bonds in a polypeptide expressed in a recombinant host cell, comprising, following fermentation, adding selenite and/or its salts or derivatives to a harvest solution of the recombinant host cell, wherein the disulfide bond in the polypeptide remains non-reduced.
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公开(公告)号:US11542341B2
公开(公告)日:2023-01-03
申请号:US16782015
申请日:2020-02-04
IPC分类号: C07K16/42 , C07K14/47 , G01N33/564 , G01N33/68 , A61P9/10 , C07K16/06 , C07K16/18 , A61K49/00 , G01N33/53
摘要: In various embodiments methods are provided for identifying a mammal having an elevated risk for an adverse cardiac event (e.g. an MI) and/or determining the prognosis for the mammal. In certain embodiments the methods comprise determining, or causing to be determined, the presence and/or level of antibodies that bind a malondialdehyde acetaldehyde adduct (MAA adduct) in a biological sample from the mammal, where an elevated level of anti-MAA adduct antibodies, as compared to the level found in a normal healthy mammal is an indicator that that said mammal has one or more atherosclerotic lesions and/or is at elevated risk for a myocardial infarction.
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